Information on EC 3.2.1.104 - steryl-beta-glucosidase

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The expected taxonomic range for this enzyme is: Sinapis alba

EC NUMBER
COMMENTARY hide
3.2.1.104
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RECOMMENDED NAME
GeneOntology No.
steryl-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cholesteryl-beta-D-glucoside + H2O = D-glucose + cholesterol
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
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SYSTEMATIC NAME
IUBMB Comments
cholesteryl-beta-D-glucoside glucohydrolase
Acts on glucosides of cholesterol and sitosterol, but not on some related sterols such as coprostanol.
CAS REGISTRY NUMBER
COMMENTARY hide
69494-88-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cholesteryl beta-D-glucoside + H2O
D-glucose + cholesterol
show the reaction diagram
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-
-
-
?
p-nitrophenyl beta-D-glucoside + H2O
D-glucose + p-nitrophenol
show the reaction diagram
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-
-
-
?
sitosteryl beta-D-glucoside + H2O
D-glucose + sitosterol
show the reaction diagram
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-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-lyso-phosphatidylcholine
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-
2-lyso-phosphatidylethanolamine
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5alpha-cholestanol
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hydrolysis of cholesteryl glucoside
campesterol
-
hydrolysis of cholesteryl glucoside
cholesterol
-
hydrolysis of cholesteryl glucoside
ethanol
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39% inhibition at a concentration of 50%, hydrolysis of p-nitrophenyl beta-D-glucoside
gluconolactone
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50% inhibition at 4 mM, hydrolysis of cholesteryl beta-D-glucoside. 50% inhibition at 2 mM, hydrolysis of p-nitrophenyl beta-D-glucoside
monogalactosyldiacylglycerol
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phosphatidylcholine
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phosphatidylethanolamine
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sitosterol
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hydrolysis of cholesteryl glucoside
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cholesteryl acetate
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150% stimulation at 0.1 mM
dithiothreitol
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45% stimulation at 0.1 mM, hydrolysis of cholesteryl beta-D-glucoside. No effect on hydrolysis of p-nitrophenyl beta-D-glucoside
ethanol
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64% stimulation at a concentration of 20%, hydrolysis of cholesteryl beta-D-glucoside. 65% stimulation at a concentration of 20%, hydrolysis of p-nitrophenyl beta-D-glucoside
methyl stearate
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150% stimulation at 0.1 mM
phytol
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200% stimulation at 0.002 mM
Tripalmitoylglycerol
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145% stimulation at 0.1 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.3621
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2 - 5.6
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hydrolysis of cholesteryl beta-D-glucoside or p-nitrophenyl beta-D-glucoside
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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hydrolysis of cholesteryl beta-D-glucoside
60
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hydrolysis of p-nitrophenyl beta-D-glucoside
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
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gel filtration
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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