Information on EC 3.13.1.1 - UDP-sulfoquinovose synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.13.1.1
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RECOMMENDED NAME
GeneOntology No.
UDP-sulfoquinovose synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of C-S bond
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hydrolysis of sulfonic acid
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Amino sugar and nucleotide sugar metabolism
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Glycerolipid metabolism
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sulfoquinovosyl diacylglycerol biosynthesis
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sulfolipid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
UDP-6-sulfo-6-deoxyglucose sulfohydrolase
Requires NAD+, which appears to oxidize UDP-alpha-D-glucose to UDP-4-dehydroglucose, which dehydrates to UDP-4-dehydro-6-deoxygluc-5-enose, to which sulfite is added. The reaction is completed when the substrate is rehydrogenated at C-4. The enzyme from Arabidopsis thaliana is specific for UDP-Glc and sulfite.
CAS REGISTRY NUMBER
COMMENTARY hide
337378-74-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
strain CECT5279, gene dszB
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Manually annotated by BRENDA team
strain CECT5279, gene dszB
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Manually annotated by BRENDA team
gene sqdB
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Manually annotated by BRENDA team
strains DS-3, IGTS8, and KA 2-5-1, gene dszB
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Manually annotated by BRENDA team
gene SQD1
UniProt
Manually annotated by BRENDA team
gene agl3, or Saci0423, of the sulfoquinovose biosynthesis operon
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-hydroxybiphenyl + sulfite
2'-hydroxybiphenyl-2-sulfinate + H2O
show the reaction diagram
UDP-alpha-D-glucose + sulfite
UDP-alpha-D-sulfoquinovopyranose + H2O
show the reaction diagram
UDP-alpha-D-sulfoquinovopyranose + H2O
UDP-alpha-D-glucose + sulfite
show the reaction diagram
the enzyme catalyzes the reverse reaction
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?
UDP-glucose + ?
UDP-sulfoquinovose + H2O
show the reaction diagram
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first step of sulfolipid biosynthesis, unknown sulfur donor
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?
UDP-glucose + sulfite
UDP-6-sulfoquinovose + H2O
show the reaction diagram
UDP-glucose + sulfite
UDP-sulfoquinovose + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-hydroxybiphenyl + sulfite
2'-hydroxybiphenyl-2-sulfinate + H2O
show the reaction diagram
UDP-alpha-D-glucose + sulfite
UDP-alpha-D-sulfoquinovopyranose + H2O
show the reaction diagram
UDP-alpha-D-sulfoquinovopyranose + H2O
UDP-alpha-D-glucose + sulfite
show the reaction diagram
C0LIR3
the enzyme catalyzes the reverse reaction
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?
UDP-glucose + ?
UDP-sulfoquinovose + H2O
show the reaction diagram
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first step of sulfolipid biosynthesis, unknown sulfur donor
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?
UDP-glucose + sulfite
UDP-6-sulfoquinovose + H2O
show the reaction diagram
Q84KI6
key enzyme in formation of the sulfolipid head group precursor UDP-sulfoquinovose, sulfoquinovosyldiacylglycerol is a polar lipid present in photosynthetic membranes, enzyme is complexed in vivo with accessory proteins
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?
UDP-glucose + sulfite
UDP-sulfoquinovose + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sulfite
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increasing concentrations beyond 0.1 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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sulfate, APS, PAPS, sulfite, thiosulfate, sufide or sulfoglutathione are tested to determine whether they could stimulate the formation of UDP-sulfoquinovose
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
sulfite
0.15
UDP-glucose
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00167 - 0.1
UDP-glucose
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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desulfurization activity in strain CECT5279 over time in vivo
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
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pH 7.0: about 50% of maximal activity, pH 10.0: about 35% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45500
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recombinant protein, lacking the transit peptide
46700
x * 46700, calculated from sequence
250000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 46700, calculated from sequence
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion results in bipyramidal crystals, C-2-symmetric homodimer, the protein has a bidomain structure, catalytic site is located in the cleft formed between the two lobes
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 25 mM NaH2PO4, pH 7.5, 300 mM NaCl, 20% glycerol
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4°C, 50 mM MES buffer, pH 6.5, 10 mM UDP-glucose, stable for 10 days
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4°C, 50 mM MES buffer, pH 6.5, stable for 2-3 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; native enzyme from chloroplasts, recombinant enzyme from Escherichia coli
native enzyme from the chloroplast stroma. Ferredoxin-dependent glutamate synthase, FdGOGAT, co-purifies and is tightly associated with SQD1
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Ni2+-nitrilotriacetic acid column
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cDNA isolation and functional expression in Escherichia coli; expression in Escherichia coli
expressed in Escherichia coli
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expression in Escherichia coli; gene agl3, DNA sequencing and analysis of gene agl3 as part of a gene cluster involved in the biosynthesis of sulfoquinovose and important for the assembly of the S-layer N-glycans, expression in Sulfolobus solfataricus agl3 deletion mutant under the control of a maltose inducible promoter using vector pSVA1450, the expression of agl3 leads to the restoration of the production of full-length glycan
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gene dszB, DszB expression pattern in Pseudomonas putida strain CECT5279
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gene dszB, organized in the dsz operon, DNA and amino acid sequence determination and analysis, genetic organization and expression analysis, recombinant expression in recombinant Rhodococcus erythropolis strain CGMCC 4.1491, that is dsz-deficient
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gene SQD1, recombinant expression in Escherichia coli
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gene SQD1, relative enzyme expression analysis
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
SQD1 transcript and protein levels are significantly up-regulated in response to sulfite injection into leaves of dark-stressed wild-type plants or into wild-type and SO Ri mutant leaves grown under a natural light regimes
the UDP-sulfoquinovose synthase in Solanum lycopersicum wild-type leaves is are downregulated during extended darkness, the expression of chloroplast-localized SQD1 transcript decreases during dark stress and recovers upon return to light in both wild-type and SO Ri plant. SQD1 transcript and protein levels are significantly up-regulated in response to sulfite injection into leaves of dark-stressed wild-type plants or into wild-type and SO Ri mutant leaves grown under a natural light regimes
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E147A
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strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
H95A
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inactive mutant enzyme
K186A
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impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
M145A
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inactive mutant enzyme
R101A
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inactive mutant enzyme
T144A
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mutant enzyme with fully retained activity, conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Y148A
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strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
Y182A
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impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
E147A
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strongly impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
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H95A
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inactive mutant enzyme
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K186A
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impaired conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
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R101A
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inactive mutant enzyme
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T144A
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mutant enzyme with fully retained activity, conversion of UDP-D-glucose to UDP-D-glucose-5,6-ene
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additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
energy production
environmental protection
medicine
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sulfolipids, in which synthesis the enzyme is involved, promise anti-tumor and anti-HIV therapeutics based on their inhibition of DNA polymerase and reverse transcriptase
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