Information on EC 3.12.1.1 - trithionate hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.12.1.1
-
RECOMMENDED NAME
GeneOntology No.
trithionate hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
trithionate + H2O = thiosulfate + sulfate + 2 H+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfate bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
-
-
Sulfur metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
trithionate thiosulfohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
115004-90-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATSB22
-
-
Manually annotated by BRENDA team
strain ATSB22
-
-
Manually annotated by BRENDA team
strain ATSB10
-
-
Manually annotated by BRENDA team
strain ATSB10
-
-
Manually annotated by BRENDA team
Halothiobacillus sp.
strain ATSB2
-
-
Manually annotated by BRENDA team
strain ATSB2
-
-
Manually annotated by BRENDA team
strain ATSB20
-
-
Manually annotated by BRENDA team
strain ATSB20
-
-
Manually annotated by BRENDA team
strain ATSB31
-
-
Manually annotated by BRENDA team
strain ATSB26
-
-
Manually annotated by BRENDA team
strain ATSB26
-
-
Manually annotated by BRENDA team
strain ATSB30
-
-
Manually annotated by BRENDA team
strain ATSB30
-
-
Manually annotated by BRENDA team
strain ATSB28
-
-
Manually annotated by BRENDA team
strain ATSB28
-
-
Manually annotated by BRENDA team
strains ATSB13 229 and ATSB16
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
trithionate + H2O
sulfate + thiosulfate
show the reaction diagram
trithionate + H2O
thiosulfate + sulfate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
trithionate + H2O
sulfate + thiosulfate
show the reaction diagram
trithionate + H2O
thiosulfate + sulfate
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
-
-
tetrathionate
-
-
trithionate
-
substrate inhibition, Ki: 6 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sulfate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.07 - 0.27
trithionate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.008 - 0.014
-
crude extract
0.038
-
partially purified enzyme after stored for 2-3 d at 4°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 7.5
-
active above pH 1 and below pH 7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
-
20% of maximum activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
-
SDS-PAGE
99000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
-
4 * 34000
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
activity lost after 36 days
30
-
40% activity after 97 days
70
-
activity lost after 27 days
additional information
-
no loss of activity by frequent freezing and thawing
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE