Information on EC 3.11.1.3 - phosphonopyruvate hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.11.1.3
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RECOMMENDED NAME
GeneOntology No.
phosphonopyruvate hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-phosphonopyruvate + H2O = pyruvate + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Phosphonate and phosphinate metabolism
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CAS REGISTRY NUMBER
COMMENTARY hide
266360-38-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain Pal6
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Manually annotated by BRENDA team
strain Pal6
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-phosphonopyruvate + H2O
pyruvate + phosphate
show the reaction diagram
phosphoenolpyruvate + H2O
enolpyruvate + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-phosphonopyruvate + H2O
pyruvate + phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
5 mM, 1.5fold activation
Cu2+
-
5 mM, 1.2fold activation
Mn2+
activates, 0.00073 mM
Zn2+
-
5 mM, 1.8fold activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-phosphonopropionic acid
Aminomethylphosphonic acid
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10 mM, 19% inhibition
Ca2+
competitive
Cu2+
5 mM, 41% loss of activity
Fe2+
-
5 mM, 94% inhibition
glyphosate
-
10 mM, 23% inhibition
hydroxymethylphosphonic acid
5 mM, 25% loss of activity
oxalate
competitive
phosphoenolpyruvate
competitive
Phosphonoacetate
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10 mM, 30% inhibition
phosphonobutyric acid
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10 mM, 14% inhibition
Phosphonoformate
-
10 mM, 37% inhibition
Phosphonoformic acid
5 mM, 76% loss of activity
sulfopyruvate
competitive
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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phosphonopyruvate and phosphonoalanine induce the enzyme in strain Pal2
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.005 - 0.72
3-phosphonopyruvate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
105 - 121
3-phosphonopyruvate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.032
Ca2+
25C, pH 7.5
2
oxalate
25C, pH 7.5
0.21
phosphoenolpyruvate
25C, pH 7.5
0.017
sulfopyruvate
25C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.35
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Variovorax sp. (strain Pal2)
Variovorax sp. (strain Pal2)
Variovorax sp. (strain Pal2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
4 * 31000, SDS-PAGE
31187
2 * 31187, mass spectrometry
63000
gel filtration
110000
gel filtrration
232000
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non-denaturing PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method, enzyme in unbound state and in complex with Mg2+ and the inhibitor oxalate
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
10 min, loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis of cell-free extract for 24 h at 4C results in a 72% loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 10 weeks, 54% loss of activity
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-20C, 25% glycerol, 6 months, about 20% loss of activity
20, 50% loss of activity after 3 days, complete loss of activity after 6 days
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4C, 30 days, 38% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene palA, DNA and amino acid sequence determination and analysis, the palA gene is organized with four other structural genes, genes palA, palB, palC, palD and palE, in the 2-amino-3-phosphonopropionic acid (phosphonoalanine)-degradative operon, overview. Expression of GFP-tagged PalA, with or without coexpression of the other genes of the operon, in Pseudomonas putida strain KT2440
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE