Information on EC 3.10.1.2 - cyclamate sulfohydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.10.1.2
-
RECOMMENDED NAME
GeneOntology No.
cyclamate sulfohydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cyclohexylsulfamate + H2O = cyclohexylamine + sulfate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfur-nitrogen bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Caprolactam degradation
-
-
Microbial metabolism in diverse environments
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-
SYSTEMATIC NAME
IUBMB Comments
cyclohexylsulfamate sulfohydrolase
Also readily hydrolyses aliphatic sulfamates with 3 to 8 carbons.
CAS REGISTRY NUMBER
COMMENTARY hide
52228-00-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain K
-
-
Manually annotated by BRENDA team
strain K
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-(n-amyl)sulfamate + H2O
n-amylamine + sulfate
show the reaction diagram
N-(n-hexyl)sulfamate + H2O
n-hexylamine + sulfate
show the reaction diagram
N-(n-octyl)sulfamate + H2O
n-octylamine + sulfate
show the reaction diagram
N-(n-propyl)sulfamate + H2O
n-propylamine + sulfate
show the reaction diagram
N-cyclohexylsulfamate + H2O
cyclohexylamine + sulfate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
-
-
Cyclohexylamine
-
-
EDTA
-
metal ions reverse inhibition
iodoacetic acid
-
-
Na2B4O7
-
-
Na2CO3
-
-
Na2SO3
-
-
p-chloromercuribenzoate
-
-
phosphate
-
-
potassium citrate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5
cyclamate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 6.7
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 7.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
2 h without loss of activity
60
-
40% loss of activity after 20 min
70
-
completely inactivated after 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
gelatin is effective stabilizer
-
in Tris buffer minor loss of activity than in phosphate buffer
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.04 M Tris-HCl buffer, pH 7.2, 0.2% gelatin, 3 months, 10% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE