Information on EC 3.1.99.B4 - DNase xthA

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Archaeoglobus fulgidus

EC NUMBER
COMMENTARY hide
3.1.99.B4
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
DNase xthA
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
DNA is digested to fragments between 100 and 500 bp
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
fragments of DNA
show the reaction diagram
the DNA is degraded relatively fast resulting in an end product of approximately half the length of the initial substrate. The enzyme is able to degrade supercoiled plasmids and shows no preferences for blunt or recessed 3'-termini of linear double stranded DNA. Whole DNA is digested to fragments between 100 and 500 bp with increasing incubation times
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
weak activity when replacing Mg2+ with Ca2+ ions
Mg2+
weak activity when replacing Mg2+ with Ca2+ ions. Mn2+ can substitute Mg2+ at concentrations below 1 mM but results in a lower activity
Mn2+
Mn2+ can substitute Mg2+ at concentrations below 1 mM but results in a lower activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29760
calculated from sequence
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE