Information on EC 3.1.99.B2 - XPF endonuclease

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.99.B2
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
XPF endonuclease
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
The best substrate for the structure-specific endonuclease is a 3' flap followed by a nicked duplex, nicked three-way junctions, and nicked fourway junctions. The enzyme from Sulfolobus is only active in the presence of the sliding clamp PCNA (Proliferating-Cell-Nuclear-Antigen). It cleaves the replication fork or 3'-flap structures in the homodimeric form.
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
proliferating-cell-nuclear-antigen
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Sulfolobus XPF is only active in the presence of the sliding clamp PCNA (Proliferating-Cell-Nuclear-Antigen), which is a heterotrimer in this organism. Interactions with two of the three subunits of PCNA are mediated via a C-terminal interaction motif. The PCNA-XPF complex acts as a structure-specificnuclease on a range of DNA flap, bubble and junction substrates
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystal structure of the homodimeric enzyme alone and bound to 15-mer dsDNA
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three different crystal forms of the enzyme are grown in trigonal, monoclinic and triclinic systems. The trigonal crystals diffract to 2.8 A and are grown in the presence of double-stranded DNA. Monoclinic crystals are grown without DNA and diffract to 3.2 A
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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