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Information on EC 3.1.99.B1 - flap endonuclease-1 and Organism(s) Sulfurisphaera tokodaii and UniProt Accession Q976H6

for references in articles please use BRENDA:EC3.1.99.B1
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
            
                3.1.99.B1 flap endonuclease-1
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This record set is specific for:
Sulfurisphaera tokodaii
UNIPROT: Q976H6
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Word Map
The taxonomic range for the selected organisms is: Sulfurisphaera tokodaii
The expected taxonomic range for this enzyme is: Archaea, Eukaryota
Reaction Schemes
endonucleases that remove 5' DNA sequences from a DNA structure called a DNA flap. The DNA flap structure occurs in double-stranded DNA containing a single-stranded break where the 5' portion of the downstream strand is too long and overlaps the 3' end of the upstream strand. Flap endonucleases cleave the downstream strand of the overlap flap structure precisely after the first base-paired nucleotide, creating a ligatable nick.
endonucleases that remove 5' DNA sequences from a DNA structure called a DNA flap. The DNA flap structure occurs in double-stranded DNA containing a single-stranded break where the 5' portion of the downstream strand is too long and overlaps the 3' end of the upstream strand. Flap endonucleases cleave the downstream strand of the overlap flap structure precisely after the first base-paired nucleotide, creating a ligatable nick
Synonyms
flap endonuclease 1, flap endonuclease, flap endonuclease-1, hfen1, t5fen, fen1 endonuclease, t5 flap endonuclease, affen, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
FEN-1
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
enzyme form StolL-FEN-1 exhibits activity in the concentration range 0.5-10 mM
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
enzyme form StolL-FEN-1 exhibits activity in the pH-range 5-9
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
x * 41000, enzyme form StoL-FEN-1, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 41000, enzyme form StoL-FEN-1, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
complete inactivation
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, in 50% glycerol solution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichi coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Horie, M.; Fukui, K.; Xie, M.; Kageyama, Y.; Hamada, K.; Sakihama, Y.; Sugimori, K.; Matsumoto, K.
The N-terminal region is important for the nuclease activity and thermostability of the flap endonuclease-1 from Sulfolobus tokodaii
Biosci. Biotechnol. Biochem.
71
855-865
2007
Sulfurisphaera tokodaii (Q976H6), Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7 (Q976H6)
Manually annotated by BRENDA team