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Information on EC 3.1.8.2 - diisopropyl-fluorophosphatase and Organism(s) Rattus norvegicus and UniProt Accession Q03336

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.8 Phosphoric-triester hydrolases
                3.1.8.2 diisopropyl-fluorophosphatase
IUBMB Comments
Acts on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including 'nerve gases'). Inhibited by chelating agents; requires divalent cations. Related to EC 3.1.8.1 aryldialkylphosphatase.
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This record set is specific for:
Rattus norvegicus
UNIPROT: Q03336
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
diisopropylfluorophosphatase, somanase, opa anhydrolase, ptes5, diisopropyl-fluorophosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
senescence marker protein-30
-
DFPase
di-isopropylphosphorofluoridate fluorohydrolase
-
-
-
-
dialkylfluorophosphatase
-
-
-
-
diisopropyl phosphorofluoridate hydrolase
-
-
-
-
diisopropylfluorophosphatase
-
-
-
-
diisopropylfluorophosphonate dehalogenase
-
-
-
-
diisopropylphosphofluoridase
-
-
-
-
isopropylphosphorofluoridase
-
-
-
-
OPA anhydrase
-
-
-
-
OPA anhydrolase
-
-
-
-
OPAA
-
-
-
-
OPAA-2
-
-
-
-
organophosphate acid anhydrase
-
-
-
-
organophosphorus acid anhydrolase
-
-
-
-
senescence marker protein-30
-
-
somanase
-
-
-
-
tabunase
-
-
-
-
additional information
SMP30 does not belong to the PON family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric triester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
diisopropyl-fluorophosphate fluorohydrolase
Acts on phosphorus anhydride bonds (such as phosphorus-halide and phosphorus-cyanide) in organophosphorus compounds (including 'nerve gases'). Inhibited by chelating agents; requires divalent cations. Related to EC 3.1.8.1 aryldialkylphosphatase.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-18-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diisopropyl phosphofluoridate + H2O
diisopropyl phosphate + fluoride
show the reaction diagram
phenyl acetate + H2O
phenol + acetate
show the reaction diagram
-
-
-
?
diisopropyl fluorophosphate + H2O
diisopropyl phosphate + fluoride
show the reaction diagram
diisopropyl phosphorofluoridate + H2O
diisopropyl phosphate + fluoride
show the reaction diagram
pH 8.0, 25°C, 1 mM MgCl2
-
-
?
phenyl acetate + H2O
phenol + acetate
show the reaction diagram
pH 8.0, 25°C, 1 mM MgCl2
-
-
?
soman + H2O
?
show the reaction diagram
-
best substrate
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
diisopropyl phosphofluoridate + H2O
diisopropyl phosphate + fluoride
show the reaction diagram
SMP30 is important in diisopropyl phosphofluoridate detoxification
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
slightly activates the hydrolysis of diisopropyl phosphofluoridate
Co2+
fully activates the hydrolysis of diisopropyl phosphofluoridate
Mg2+
fully activates the hydrolysis of diisopropyl phosphofluoridate
Mn2+
fully activates the hydrolysis of diisopropyl phosphofluoridate
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mn2+
-
activates
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CdCl2
1 mM, stimulates hydrolysis to a lesser extent, 38% activity, compared to MgCl2
CoCl2
1 mM, stimulates hydrolysis well, 102% activity, compared to MgCl2
MgCl2
1 mM, stimulates hydrolysis well, 100% activity
MnCl2
1 mM, stimulates hydrolysis well, 100% activity
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.527
purified SMP30, substrate phenyl acetate
1.512
purified SMP30, substrate diisopropyl phosphofluoridate
0.527
with phenyl acetate as substrate, pH 8.0, 25°C, 1 mM MgCl2
0.998
with diisopropyl phosphorofluoridate as substrate, pH 8.0, 25°C, 1 mM MgCl2
1.512
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SMP30, gene rgn; gene rgn
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RGN_RAT
299
0
33390
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
x * 34000, SMP30, SDS-PAGE
34000
x * 34000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 34000, SMP30, SDS-PAGE
?
x * 34000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
SMP30 49fold from liver by anion exchange and hydrophobic interaction chromatography, and gel filtration
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hoskin, F.C.G.; Prusch, R.D.
Characterization of a DFP-hydrolyzing enzyme in squid posterior salivary gland by use of Soman, DFP and manganous ion
comp. Biochem. Physiol. C
75
17-20
1983
Loligo sp., Rattus norvegicus
Manually annotated by BRENDA team
Kondo, Y.; Ishigami, A.; Kubo, S.; Handa, S.; Gomi, K.; Hirokawa, K.; Kajiyama, N.; Chiba, T.; Shimokado, K.; Maruyama, N.
Senescence marker protein-30 is a unique enzyme that hydrolyzes diisopropyl phosphorofluoridate in the liver
FEBS Lett.
570
57-62
2004
Mus musculus, Rattus norvegicus, Rattus norvegicus (Q03336)
Manually annotated by BRENDA team