Information on EC 3.1.7.9 - isotuberculosinol synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY hide
3.1.7.9
-
RECOMMENDED NAME
GeneOntology No.
isotuberculosinol synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
tuberculosinyl diphosphate + H2O = (13S)-isotuberculosinol + diphosphate
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
tuberculosinyl diphosphate diphosphohydrolase (isotuberculosinol forming)
Only found in species of Mycobacterium that cause tuberculosis. In addition, it also gives tuberculosinol in 1:1 mixture, cf. EC 3.1.7.8, tuberculosinol synthase. The isotuberculosinol form was a 3:1 mixture of the 13S and 13R forms, respectively.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
copalyl diphosphate + H2O
(+)-copalol + manool + 13-epi-manool + diphosphate
show the reaction diagram
-
-
40% (+)-copalol, 12% manool and 15% 13-epi-manool
-
?
ent-copalyl diphosphate + H2O
(-)-ent copalol + ent-13-epi-manool + ent-manool + diphosphate
show the reaction diagram
-
-
22% (-)-ent copalol, 8% ent-13-epi-mancol, and 42% ent-manool
-
?
syn-copalyl diphosphate + H2O
syn-copalol + (13R)-vitexifolin + (13S)-vitexifolin + diphosphate
show the reaction diagram
-
-
16% syn-copalol, 3% (13R)-vitrxifolin and 59% (13S)-vitexifolin
-
?
tuberculosinyl diphosphate + H2O
(13R)-isotuberculosinol + (13S)-isotuberculosinol + diphosphate
show the reaction diagram
tuberculosinyl diphosphate + H2O
(13S)-isotuberculosinol + diphosphate
show the reaction diagram
tuberculosinyl diphosphate + H2O
tuberculosinol + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
17% activity at 1 mM
Fe2+
-
20% activity at 1 mM
Mn2+
-
13% activity at 1 mM
Ni2+
-
35% activity at 1 mM
Zn2+
-
10% activity at 1 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
-
no activity at temperatures higher than 50°C
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
2 * 30000, SDS-PAGE
60000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme and complexes with inhibitor BPH-629, and mutants Y51F with TPP, mutant Y51F/Y90F with TPP, to 1.8-2.7 A resolution
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, amylase column chromatography, and G3000SWXL gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D34A
compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 60% tuberculosinol
D81N
-
24% activity compared to the wild type enzyme
D81N/D82N
-
inactive
D81N/D85N
-
inactive
D82N
-
21% activity compared to the wild type enzyme
D82N/D85N
-
inactive
D85N
-
37% activity compared to the wild type enzyme
R38A
compared to wild-type, mutant produces about 5% (13S)-isotuberculosinol and 140% tuberculosinol
Y51F
production of both (13S)-isotuberculosinol and tuberculosinol is reduced
Y51F/Y90F
no enzymic activity
Y90F
compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 49% tuberculosinol