Information on EC 3.1.7.8 - tuberculosinol synthase

Word Map on EC 3.1.7.8
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Mycobacterium tuberculosis

EC NUMBER
COMMENTARY hide
3.1.7.8
-
RECOMMENDED NAME
GeneOntology No.
tuberculosinol synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
tuberculosinyl diphosphate + H2O = tuberculosinol + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tuberculosinol biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
tuberculosinyl diphosphate diphosphohydrolase (tuberculosinol forming)
Only found in species of Mycobacterium that cause tuberculosis. In addition, it also gives isotuberculosinol in 1:1 mixture, cf. EC 3.1.7.9, isotuberculosinol synthase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
copalyl diphosphate + H2O
(+)-copalol + manool + 13-epi-manool + diphosphate
show the reaction diagram
ent-copalyl diphosphate + H2O
(-)-ent copalol + ent-13-epi-manool + ent-manool + diphosphate
show the reaction diagram
syn-copalyl diphosphate + H2O
syn-copalol + (13R)-vitexifolin + (13S)-vitexifolin + diphosphate
show the reaction diagram
tuberculosinyl diphosphate + H2O
(13S)-isotuberculosinol + diphosphate
show the reaction diagram
tuberculosinyl diphosphate + H2O
tuberculosinol + diphosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
17% activity at 1 mM
Fe2+
-
20% activity at 1 mM
Mn2+
-
13% activity at 1 mM
Ni2+
-
35% activity at 1 mM
Zn2+
-
10% activity at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0254 - 0.0396
tuberculosinyl diphosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000998 - 0.000517
tuberculosinyl diphosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00398 - 0.0191
tuberculosinyl diphosphate
5064
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 45
-
no activity at temperatures higher than 50°C
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
2 * 30000, SDS-PAGE
60000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme and complexes with inhibitor BPH-629, and mutants Y51F with TPP, mutant Y51F/Y90F with TPP, to 1.8-2.7 A resolution
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, amylase column chromatography, and G3000SWXL gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D34A
compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 60% tuberculosinol
D81N
-
24% activity compared to the wild type enzyme
D81N/D82N
-
inactive
D81N/D85N
-
inactive
D82N
-
21% activity compared to the wild type enzyme
D82N/D85N
-
inactive
D85N
-
37% activity compared to the wild type enzyme
R38A
compared to wild-type, mutant produces about 5% (13S)-isotuberculosinol and 140% tuberculosinol
Y51F
production of both (13S)-isotuberculosinol and tuberculosinol is reduced
Y51F/Y90F
no enzymic activity
Y90F
compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 49% tuberculosinol
D34A
-
compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 60% tuberculosinol
-
R38A
-
compared to wild-type, mutant produces about 5% (13S)-isotuberculosinol and 140% tuberculosinol
-
Y51F
-
production of both (13S)-isotuberculosinol and tuberculosinol is reduced
-
Y51F/Y90F
-
no enzymic activity
-
Y90F
-
compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 49% tuberculosinol
-
D81N
-
24% activity compared to the wild type enzyme
-
D81N/D82N
-
inactive
-
D81N/D85N
-
inactive
-
D82N
-
21% activity compared to the wild type enzyme
-
D85N
-
37% activity compared to the wild type enzyme
-