Information on EC 3.1.7.5 - geranylgeranyl diphosphate diphosphatase

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The expected taxonomic range for this enzyme is: Croton stellatopilosus

EC NUMBER
COMMENTARY hide
3.1.7.5
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RECOMMENDED NAME
GeneOntology No.
geranylgeranyl diphosphate diphosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranylgeranyl diphosphate + H2O = geranylgeraniol + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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plaunotol biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphohydrolase
Involved in the biosynthesis of plaunotol. There are two isoenzymes with different ion requirements. Neither require Mg2+ but in addition PII is inhibited by Zn2+, Mn2+ and Co2+. It is not known which isoenzyme is involved in plaunotol biosynthesis.
CAS REGISTRY NUMBER
COMMENTARY hide
160995-43-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
farnesyl diphosphate + H2O
farnesol + diphosphate
show the reaction diagram
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11% of the activity with geranylgeranyl diphosphate, isoenzyme PI. 23% of the activity with geranylgeranyl diphosphate, isoenzyme PII
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?
geranyl diphosphate + H2O
geraniol + diphosphate
show the reaction diagram
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7% of the activity with geranylgeranyl diphosphate, isoenzyme PI. 10% of the activity with geranylgeranyl diphosphate, isoenzyme PII
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?
geranylgeranyl diphosphate + H2O
geranylgeraniol + diphosphate
show the reaction diagram
geranylgeranyl diphosphate + H2O
geranylgeraniol + phosphate
show the reaction diagram
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formation of geranylgeraniol from geranylgeranyl diphosphate proceeds in the chloroplasts via two successive monodephosphorylation reactions
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?
geranylgeranyl phosphate + H2O
geranylgeraniol + phosphate
show the reaction diagram
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?
additional information
?
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no activity with isopentenyl diphosphate, isoenzyme PI and PII
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranylgeranyl diphosphate + H2O
geranylgeraniol + diphosphate
show the reaction diagram
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the enzyme is involved in the biosynthesis of plaunotol, a commercial anti-peptic acyclic diterpenoid
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
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1.0 mM, 18% inhibition of isoenzyme PI, slight activation (1.1fold) of isoenzyme PII
additional information
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both activities (isoenzyme I and II) are Mg2+-independent
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Co2+
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1.0 mM, complete inhibition of isoenzyme PII, 21% inhibition of isoenzyme PI
geranylgeranyl diphosphate
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substrate inhibition above 0.2 mM, isoenzyme PII. Isoenzyme PI shows no substrate inhibition
K+
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1.0 mM, 18% inhibition of isoenzyme PI, slight activation (1.1fold) of isoenzyme PII
Mn2+
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1.0 mM, complete inhibition of isoenzyme PII, 4% inhibition of isoenzyme PI
Na+
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1.0 mM, 23% inhibition of isoenzyme PI, 5% inhibition of isoenzyme PII
Na2MoO4
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1.0 mM, complete inhibition of isoenzyme PI and isoenzyme PII
Zn2+
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1.0 mM, complete inhibition of isoenzyme PII, no inhibition of isoenzyme PI
additional information
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isoenzyme PI shows no substrate inhibition by geranylgeranyl diphosphate, no inhibition by 1.0 mM Zn2+ or K+ and very low inhibition by 1 mM Na+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1 - 0.2
geranylgeranyl diphosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.04
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isoenzyme PI
0.35
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isoenzyme PII
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 6.5
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isoenzyme PI
6.5 - 7
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isoenzyme PII
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 7.5
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pH 5.0: about 65% of maximal activity, pH 7.0: about 60% of maximal activity, isoenzyme II; pH 5.0: about 80% of maximal activity, pH 7.0: about 45% of maximal activity, isoenzyme I
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30600
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1 * 30600, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE
34000
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isoenzyme PII, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, gel filtration
58700
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4 * 58700, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE
232000
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isoenzyme PI, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 30600, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE
tetramer
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4 * 58700, it is not known which isoenzyme (PI or PII) is involved in plaunotol biosynthesis, isoenzyme PII, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial purification of isoenzyme PI and isoenzyme PII
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information