Information on EC 3.1.7.2 - guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.7.2
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RECOMMENDED NAME
GeneOntology No.
guanosine-3',5'-bis(diphosphate) 3'-diphosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ppGpp biosynthesis
Purine metabolism
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SYSTEMATIC NAME
IUBMB Comments
guanosine-3',5'-bis(diphosphate) 3'-diphosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
70457-12-4
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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Mesh1 deletion impairs starvation resistance in Drosophila, overview
additional information
-
crucial residues for the ppGpp hydrolysis activity of Mesh1 are Arg24, Glu65, Asp66 and Asn126
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ppGpp + H2O
Gpp + diphosphate
show the reaction diagram
ppGpp + H2O
ppG + diphosphate
show the reaction diagram
pppGpp + H2O
pppG + diphosphate
show the reaction diagram
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-
-
-
r
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ppGpp + H2O
Gpp + diphosphate
show the reaction diagram
ppGpp + H2O
ppG + diphosphate
show the reaction diagram
additional information
?
-
-
only ppGpp, and no other nucleotide, is effectively hydrolyzed by human MESH1
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-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activation
Co2+
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activation
NH4+
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activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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chlorotetracycline
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Levallorphan
tetracycline
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Thiostrepton
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Uncharged tRNA
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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activation by a low moelcular weight activation factor and ATP, inhibited by levallorphan
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.41
ppGpp
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pH 8.0, 30C, KM is increased 3fold in the presence of the complex containing tRNA, ribosomes, and mRNA
additional information
additional information
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.97 - 6
ppGpp
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.46 - 9.58
ppGpp
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.46
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enzyme fragment NH 1-24
0.7
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enzyme fragment NH 1-385
52
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full-length enzyme NC 1-739
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
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calculation from sequence of the spotT gene
130000
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dimeric enzyme form, gel filtration
260000
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tetrameric enzyme form, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
fragments of the relMtb protein
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recombinant full-length enzyme from Escherichia coli
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of relSeq fragments and the expression is regulated using pBAD vectors
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expression of fragment proteins in Escherichia coli BL21(DE3)
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preparation of mutants with null alleles at both, spoT and relA
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recombinant expression of full-length enzyme in Escherichia coli and in HEK-293T cells
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recombinant expression of full-length enzyme in Escherichia coli and in S2 cells
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spotT gene
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the pET22b expression system is used
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D81A
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loss of hydrolytic activity with retention of synthesis
DElTA1-86/DELTA395-738
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contains only (p)ppGpp synthesis activity, no hydrolysis activity
DELTA182-738
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fragment contains only (p)ppGpp hydrolysis activity, no synthesis activity
DELTA395-738
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frament contains both synthesis and hydrolysis activities
G241E
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loss of synthetic activity and retention of hydrolysis
H344Y
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loss of synthetic activity and retention of hydrolysis
H80A
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loss of hydrolytic activity with retention of synthesis
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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RelMtb is a key factor in Mycobacterium tuberculosis pathogenesis by regulating th intracellular concentration of (p)ppGpp.
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