Information on EC 3.1.6.9 - chondro-4-sulfatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.6.9
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RECOMMENDED NAME
GeneOntology No.
chondro-4-sulfatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
4-deoxy-beta-D-gluc-4-enuronosyl-(1->3)-N-acetyl-D-galactosamine 4-sulfate + H2O = 4-deoxy-beta-D-gluc-4-enuronosyl-(1->3)-N-acetyl-D-galactosamine + sulfate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfuric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chondroitin sulfate degradation I (bacterial)
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dermatan sulfate degradation I (bacterial)
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SYSTEMATIC NAME
IUBMB Comments
4-deoxy-beta-D-gluc-4-enuronosyl-(1->3)-N-acetyl-D-galactosamine-4-sulfate 4-sulfohydrolase
Also acts on the saturated analogue but not on higher oligosaccharides, nor any 6-sulfates.
CAS REGISTRY NUMBER
COMMENTARY hide
9045-75-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc4SO3--beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-2-sulfate-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
show the reaction diagram
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removal of two sulfate groups
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?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
show the reaction diagram
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removal of two sulfate groups
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?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
show the reaction diagram
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removal of one sulfate group
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?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
show the reaction diagram
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removal of two sulfate groups
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?
4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc4SO3- + H2O
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid2SO3--beta-(1-3)-GalNAc6SO3--beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc6SO3-beta-(1-4)-glucuronic acid-beta-(1-3)-GalNAc
show the reaction diagram
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removal of one sulfate group
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?
4-deoxy-alpha-L-threo-hex-4-enepyranuronosyl-(1->3)-beta-D-GalNAc(4-O-sulfate)-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal(4-O-sulfate)-(1->3)-beta-D-Gal-(1->4)-D-Xyl + H2O
sulfate + 4-deoxy-alpha-L-threo-hex-4-enepyranuronosyl-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-D-Xyl
show the reaction diagram
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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the enzyme breaks down one of the products of the chondroitin lyase reaction. An upstream insertion mutant that has no detectable chondro-4-sulfatase activity is still able to grow on chondroitin sulfate, although the rate is slower than that of the wild type
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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the enzyme is a useful tool for structural studies of chondroitin sulfate and dermetan sulfate