Information on EC 3.1.6.6 - choline-sulfatase

Word Map on EC 3.1.6.6
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.6.6
-
RECOMMENDED NAME
GeneOntology No.
choline-sulfatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
choline sulfate + H2O = choline + sulfate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfuric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
choline-O-sulfate degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
choline-sulfate sulfohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9025-59-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
product of betC gene. Enzyme expression is downregulated at high salt concentrations
-
-
Manually annotated by BRENDA team
isolate from sewage with the ability to degrade detergents, e.g. SDS, gene betC
UniProt
Manually annotated by BRENDA team
V-A
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
amylose polysulfate + H2O
amylose + sulfate
show the reaction diagram
-
slightly hydrolyzed
-
-
?
choline sulfate + H2O
choline + sulfate
show the reaction diagram
choline sulfate + H2O
phosphorylcholine
show the reaction diagram
-
-
-
?
choline-O-sulfate + H2O
choline + sulfate
show the reaction diagram
phosphorylcholine + H2O
choline + phosphate
show the reaction diagram
-
less than 20% activity compared to choline sulfate as substrat
-
-
-
additional information
?
-
-
enzyme is unrelated to osmoprotection
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
choline sulfate + H2O
choline + sulfate
show the reaction diagram
choline-O-sulfate + H2O
choline + sulfate
show the reaction diagram
additional information
?
-
-
enzyme is unrelated to osmoprotection
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
-
complete inhibition
Hg2+
-
complete inhibition
Mg2+
-
slight inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
betaine
-
linear competitive inhibitor
Chloramphenicol
-
synthesis of the enzyme is repressed
choline
choline chloride
cysteine
Dimethylamine
-
uncompetitive inhibitor
dimethylaminoethanol
-
uncompetitive inhibitor
dithiothreitol
mercaptoethanol
methionine
N-ethylmaleimide
NaCl
-
slight inhibition at high concentrations
p-chloromercuribenzoate
phosphorylcholine
sulfate
sulfite
tetramethylamine
-
uncompetitive inhibitor
trimethylamine
-
uncompetitive inhibitor
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
choline
-
formation of the enzyme is induced by
Choline sulfate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.4 - 40
Choline sulfate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
lyophilized
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
175000
-
gel filtration
345000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene betC and DNA of betC and betR intergenic region, DNA and amino acid sequence determination and analysis, the betC promoter is inducible
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the putative transcriptional regulator BetR activates betC and betR transcription in the presence of choline sulfate, whilst in the absence of choline sulfate BetR represses its own transcription. BetR directly binds to betC-betR intergenic region in vitro, with higher affnity in the presence of choline sulfate as cofactor
transcription of betC and betR is not induced in the presence of high concentration of NaCl
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Show AA Sequence (982 entries)
Please use the Sequence Search for a specific query.