Information on EC 3.1.6.3 - glycosulfatase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.6.3
-
RECOMMENDED NAME
GeneOntology No.
glycosulfatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucose 6-sulfate + H2O = D-glucose + sulfate
show the reaction diagram
also acts on other sulfates of mono- and disaccharides and on adenosine 5'-sulfate
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-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfuric ester
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-
-
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SYSTEMATIC NAME
IUBMB Comments
sugar-sulfate sulfohydrolase
Also acts on other sulfates of monosaccharides and disaccharides and on adenosine 5'-sulfate.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-61-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain VIII-210; strain VIII-240
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-
Manually annotated by BRENDA team
strain VIII-210
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-
Manually annotated by BRENDA team
strain VIII-240
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-
Manually annotated by BRENDA team
sulfatase A of ox liver also has glycosulfatase activity
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-
Manually annotated by BRENDA team
enzyme production occurs only in swarm cells at low dilution rates in mucin-limited cultures
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-
Manually annotated by BRENDA team
mollusc
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-
Manually annotated by BRENDA team
common limpet
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-
Manually annotated by BRENDA team
strain RS2
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-
Manually annotated by BRENDA team
Pseudomonas carrageenovora
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-
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Manually annotated by BRENDA team
strain IX-70
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-
Manually annotated by BRENDA team
strain VIII-239
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-
Manually annotated by BRENDA team
mould
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-galactosyl-6-O-sulfate (1-4)-glucitol + H2O
beta-galactosyl-(1-4)glucitol
show the reaction diagram
D-galactose-6-O-sulfate + H2O
D-galactose + sulfate
show the reaction diagram
D-glucose-3-O-sulfate + H2O
D-glucose + sulfate
show the reaction diagram
D-glucose-6-sulfate + H2O
D-glucose + sulfate
show the reaction diagram
D-mannose-6-sulfate + H2O
D-mannose + sulfate
show the reaction diagram
-
-
-
-
?
galactose-6-sulfate + H2O
galactose + sulfate
show the reaction diagram
-
-
-
-
?
galactosylceramide sulfate + H2O
galactosylceramide + sulfate
show the reaction diagram
L-fucose 2-sulfates + H2O
L-fucose + sulfate
show the reaction diagram
-
-
-
-
?
L-fucose 3-sulfate + H2O
L-fucose + sulfate
show the reaction diagram
-
-
-
-
?
L-fucose 4-sulfate + H2O
L-fucose + sulfate
show the reaction diagram
-
-
-
-
?
lactosylceramide sulfate + H2O
lacotsylceramide + sulfate
show the reaction diagram
N-acetyl-D-glucosamine 6-sulfate + H2O
N-acetyl-D-glucosamine + sulfate
show the reaction diagram
neoarabiose 4-O-sulfate + H2O
neocarrabiose + sulfate
show the reaction diagram
Pseudomonas carrageenovora
-
-
-
?
neocarratetraose 4-O-sulfate + H2O
neocarratetraose + sulfate
show the reaction diagram
Pseudomonas carrageenovora
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-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
-
increases activity
MnCl2
-
increases activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Borate
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D-glucose
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enzyme activity is suppressed by readily fermentable carbon sources such as glucose
diphosphate
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HgCl2
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weak
nicetapone
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p-hydroxymercuribenzoate
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PO43-
Semicarbazide
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-
additional information
-
no inhibition by iodacetamide
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
-
100 mM activates
Triton X-100
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0.3% activates
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
30
D-glucose 3-O-sulfate
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-
29.4
D-glucose 3-sulfate
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17
D-glucose 6-O-sulfate
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25 - 60
glucose 6-sulfate
11.8
N-acetyl-D-glucosamine 6-sulfate
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-
additional information
additional information
Pseudomonas carrageenovora
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
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D-galactose 6-O-sulfate
5.5 - 5.6
5.7 - 5.9
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D-glucose 3-O-sulfate
7.4
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cytoplasm
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
Pseudomonas carrageenovora
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pH 6.0: about 75% of activity maximum, pH 9.0: about 5% of activity maximum
6.5 - 9
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pH 6.5: 10-20% of activity maximum, pH 9.0: 30-40% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22 - 37
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22C: 25% of activity maximum, 37C: about 35% of activity maximum
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Pseudomonas carrageenovora
-
-
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Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
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? * 30000, SDS-PAGE
38000
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? * 38000, SDS-PAGE
55000
Pseudomonas carrageenovora
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? * 55000, SDS-PAGE
58000
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2 * 58000, SDS-PAGE
79000
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gel filtration, glycosulfatase I
111000
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gel filtration
112000
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gel filtration, glycosulfatase II
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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glycosulfatase I and II differ in carbohydrate moieties
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
unstable to freezing
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 12 months, 78% of original activity
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4C, 12 months, 76% of original activity
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4C, 20 mM phosphate buffer, pH 7.4, 2-mercaptoethanol, 1-3 months, 92-74% of original activity
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4C, over P2O5, 12 months, 69% of original activity
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mycelium extract, freeze-dried, 3 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE