Information on EC 3.1.6.18 - glucuronate-2-sulfatase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.6.18
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RECOMMENDED NAME
GeneOntology No.
glucuronate-2-sulfatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of the 2-sulfate groups of the 2-O-sulfo-D-glucuronate residues of chondroitin sulfate, heparin and heparitin sulfate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfuric ester
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosaminoglycan degradation
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
polysaccharide-2-O-sulfo-D-glucuronate 2-sulfohydrolase
Does not act on iduronate 2-sulfate residues (cf. EC 3.1.6.13 iduronate-2-sulfatase)
CAS REGISTRY NUMBER
COMMENTARY hide
98597-45-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate + H2O
D-glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate + SO42-
show the reaction diagram
D-glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate + H2O
D-glucuronic acid 2,5-anhydro-D-talitol 6-sulfate + SO42-
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
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stimulation, over pH-range 4.2-4.8, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate, inhibits at pH 3
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
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above pH 4.2, activation over pH-range 3-4.2, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
Glucuronic acid 2,5-anhydro-D-mannitol 6-sulfate
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product inhibition
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Glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate
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i.e. GSMS, substrate inhibition, above 0.004 mM
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Glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate
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i.e. GSTS, substrate inhibition, above 0.004 mM
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NaH2PO4
O-alpha-Iduronic acid 2-sulfate-1,4-O-(2,5)-anhydro-D-mannitol 6-sulfate
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SO42-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.65
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liver
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.7
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20 mM sodium formate buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate as substrate
3.2
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glucuronic acid 2-sulfate-2,5-anhydro-D-talitol 6-sulfate as substrate
3.3
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60 mM sodium formate or 60 mM glycine/HCl buffer, glucuronic acid 2-sulfate-2,5-anhydro-D-mannitol 6-sulfate as substrate
additional information
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the pH/activity profile depends on buffer composition and ionic strength, particularly at lower pH-values
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 4.9
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about 90% of maximal activity at pH 3 and about half-maximal activity at pH 4.9
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6 - 4.7
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urine enzyme forms with pI: 4.6, 5.2, 5.8 and pI: below 4
5.2
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urine enzyme forms with pI: 4.6, 5.2, 5.8 and pI: below 4
5.8
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urine enzyme forms with pI: 4.6, 5.2, 5.8 and pI: below 4
6.5
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major enzyme form from human skin; skin fibroblasts, additional enzyme form 5.4, 5.8, below 4 and above 7.5
7.5
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above, major enzyme form in liver, more than 90% of liver activity, chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19500
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x * 47000 + x * 19500, enzyme from liver, SDS-PAGE
47000
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x * 47000 + x * 19500, enzyme from liver, SDS-PAGE
69000
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enzyme from liver, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, in 50 mM sodium acetate buffer, pH 5.6, 0.5 M NaCl, partially purified enzyme preparation, 10% loss of activity within 1 month, purified enzyme, t1/2: 48 h, 0.1 mg/ml bovine serum albumin stabilizes
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE