Information on EC 3.1.6.17 - D-lactate-2-sulfatase

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The expected taxonomic range for this enzyme is: Pseudomonas syringae

EC NUMBER
COMMENTARY hide
3.1.6.17
-
RECOMMENDED NAME
GeneOntology No.
D-lactate-2-sulfatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(R)-2-O-sulfolactate + H2O = (R)-lactate + sulfate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of sulfuric ester
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
(R)-2-O-sulfolactate 2-sulfohydrolase
Highly specific.
CAS REGISTRY NUMBER
COMMENTARY hide
93586-05-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl sulfate + H2O
4-nitrophenol + SO42-
show the reaction diagram
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at 6% the rate of D-lactate 2-sulfate hydrolysis
-
?
D-lactate 2-sulfate + H2O
D-lactate + SO42-
show the reaction diagram
-
stereospecific, not L-lactate 2-sulfate, cleaves O-S-bond of the C-O-S-ester link
not L-lactate
?
serine-O-sulfate + H2O
serine + SO42-
show the reaction diagram
-
at 9% the rate of D-lactate 2-sulfate hydrolysis
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-
?
additional information
?
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no substrates are the sulfate esters of L-lactate, methanol, ethanol, propanol, propan-2-ol, butanol, butan-2-ol, pentanol, pentan-2-ol, pentan-3-ol, hexanol, hexan-2-ol, heptanol, heptan-2-ol, heptan-4-ol, octanol, octan-2-ol, decanol, decan-5-ol, 2-hydroxybutyrate, 2-hydroxyisobutyrate, butoxyethanol and phenoxyethanol, the 6-sulfate esters of glucosamine and N-acetylglucosamine and the 3-sulfate esters of glucosamine and N-acetylgalactosamine. No dehalogenase activity: potassium DL-chloropropionate is not a substrate
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-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-Chloropropionate
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20 mM, 8% inhibition
butan-2-yl-sulfate
-
20 mM, 25% inhibition
butyrate-2-sulfate
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20 mM, 42% inhibition
D-lactate
-
product inhibition, kinetics
D-serine-O-sulfate
-
20 mM, 11% inhibition
glycolate-2-sulfate
-
20 mM, 43% inhibition
Hexan-2-yl sulfate
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20 mM, 13% inhibition
L-lactate 2-sulfate
-
12 mM, 43% inhibition
pentan-3-yl
-
20 mM, 10% inhibition
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propan-1-yl sulfate
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20 mM, 22% inhibition
propan-2-yl-sulfate
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20 mM, 18% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0067
D-Lactate 2-sulfate
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pH 7.5, 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16.3
L-lactate-2-sulfate
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pH 7.5, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.9
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pH 7.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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0.2 M Tris-HCl buffer
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
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1 * 55000, Pseudomonas syringae, SDS-PAGE
59800
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PAGE, gel filtration yields a higher MW, presumably due to aggregation
89000
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gel filtration, high MW presumably due to aggregation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 55000, Pseudomonas syringae, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
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pH 7.5, stable up to, 5 min
50
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pH 7.5, 5 min, inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE