Information on EC 3.1.5.1 - dGTPase

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The expected taxonomic range for this enzyme is: Enterobacteriaceae

EC NUMBER
COMMENTARY hide
3.1.5.1
-
RECOMMENDED NAME
GeneOntology No.
dGTPase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
dGTP + H2O = deoxyguanosine + triphosphate
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
purine metabolism
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Purine metabolism
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SYSTEMATIC NAME
IUBMB Comments
dGTP triphosphohydrolase
Also acts on GTP.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-63-2
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
blockage of the alternative dUMP phosphatase pathway for deoxyribose-1-phosphate generation greatly exacerbates the severity of thymine starvation in enriched media, and under these conditions the dgt pathway becomes crucial in protecting the cells against thymineless death
physiological function
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the minimal amount of thymine required for growth of thymine-requiring (thyA) strains decreases with increased expression level of the dgt gene. As expected, this dgt-mediated effect is dependent on the DeoD purine nucleoside phosphorylase. ThyA strains experience growth difficulties upon nutritional shift-up, the dgt gene facilitates adaptation to the new growth conditions
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
8-bromo-dGTP + H2O
8-bromo-deoxyguanosine + triphosphate
show the reaction diagram
-
the enzyme degrades 8-bromo-dGTP at a comparable rate to dGTP
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-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
GTP + H2O
guanosine + triphosphate
show the reaction diagram
additional information
?
-
-
the enzyme has little activity toward rNTPs including GTP, 8-oxo-dGTP is a poor substrate
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-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation 17% the rate of Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2 protein
-
interaction is modulated by dGTP and polynucleotides; of bacteriophage T7
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2-mercaptoethanol
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prior incubation, loss of thermostability
dGTP
-
competitive inhibitor
dithiothreitol
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prior incubation, 12% of activity remains, loss of thermostability
GTP
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competitive inhibitor
NaCl
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inhibition of binding to single or double stranded DNA
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
single stranded DNA
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-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.005
dGTP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4000
dGTP
Escherichia coli
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
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70% activity at pH 9.2
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
230000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
initial conditions, vapor diffusion method
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
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reversible loss of activity at low protein concentrations
55 - 60
60
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loss of thermostability in presence of reducing agents, 5% of activity remains
65 - 70
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90% denatured after 1 h
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
more stable at higher protein concentrations
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0 to -20°C, 0.1 mg/ml protein concentration, 30 days, 20% and 60% loss of activity
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stable at -20°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
16000 fold to homogeneity
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16000 fold to homogeneity; high affinity for DNA
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high affinity for DNA
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partial
partial, no precipitation with nucleic acids
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recombinant protein to homogeneity
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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