Information on EC 3.1.5.1 - dGTPase

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The expected taxonomic range for this enzyme is: Enterobacteriaceae

EC NUMBER
COMMENTARY
3.1.5.1
-
RECOMMENDED NAME
GeneOntology No.
dGTPase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
dGTP + H2O = deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
purine metabolism
-
-
Purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
dGTP triphosphohydrolase
Also acts on GTP.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
deoxy-GTPase
-
-
-
-
deoxyguanosine 5-triphosphate triphosphohydrolase
-
-
-
-
deoxyguanosine tri-oder deoxyguanosine triphosphatase
-
-
-
-
deoxyguanosine triphosphate triphosphohydrolase
-
-
-
-
dGTP triphosphohydrolase
-
-
-
-
dGTP triphosphohydrolase
-
-
dGTPase
-
-
-
-
phosphatase, deoxyguanosine tri
-
-
-
-
dGTPase
-
-
additional information
-
50 fold increased level; C-T transition 74 bases upstream; OPTA1 mutants
CAS REGISTRY NUMBER
COMMENTARY
9025-63-2
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
-
blockage of the alternative dUMP phosphatase pathway for deoxyribose-1-phosphate generation greatly exacerbates the severity of thymine starvation in enriched media, and under these conditions the dgt pathway becomes crucial in protecting the cells against thymineless death
physiological function
-
the minimal amount of thymine required for growth of thymine-requiring (thyA) strains decreases with increased expression level of the dgt gene. As expected, this dgt-mediated effect is dependent on the DeoD purine nucleoside phosphorylase. ThyA strains experience growth difficulties upon nutritional shift-up, the dgt gene facilitates adaptation to the new growth conditions
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
8-bromo-dGTP + H2O
8-bromo-deoxyguanosine + triphosphate
show the reaction diagram
-
the enzyme degrades 8-bromo-dGTP at a comparable rate to dGTP
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
highly specific for
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
triphosphate group required
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
no activity on dGDP
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
no activity on dGDP
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
no activity on dGDP
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
no activity on dGDP
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
no activity on dGDP
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
no activity on dGDP
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
the enzyme strongly prefers dGTP among the dNTPs
-
?
GTP + H2O
guanosine + triphosphate
show the reaction diagram
-
-
-
-
GTP + H2O
guanosine + triphosphate
show the reaction diagram
-
at a lower rate than dGTP
-
?
additional information
?
-
-
the enzyme has little activity toward rNTPs including GTP, 8-oxo-dGTP is a poor substrate
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
dGTP + H2O
deoxyguanosine + triphosphate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
activation 17% the rate of Mg2+
Mg2+
-
no activity in absence of
Mg2+
-
enhances DNA binding but not required
Mn2+
-
activation 30% the rate of Mg2+
Mn2+
-
enhance DNA binding but not required
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1.2 protein
-
interaction is modulated by dGTP and polynucleotides; of bacteriophage T7
-
2-mercaptoethanol
-
prior incubation, loss of thermostability
dGTP
-
competitive inhibitor
dithiothreitol
-
prior incubation, 12% of activity remains, loss of thermostability
GTP
-
competitive inhibitor
NaCl
-
inhibition of binding to single or double stranded DNA
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
single stranded DNA
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.001
dGTP
-
-
0.002 - 0.005
dGTP
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
4000
dGTP
Escherichia coli
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5 - 8.5
-
70% activity at pH 9.2
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
230000
-
gel filtration; SDS-PAGE
135503
230000
-
enzyme is present only in enteric bacteria; gel filtration
135507
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
tetramer
-
homotetramer, 4 * 58900; SDS-PAGE
tetramer
-
allosteric interaction between subunits
tetramer
-
-
tetramer
-
homotetramer, 4 * 59400
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
initial conditions, vapor diffusion method
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0
-
reversible loss of activity at low protein concentrations
135505
55 - 60
-
full activity after 1 h
135503, 135505
55 - 60
-
full activity after 1 h
135507
60
-
loss of thermostability in presence of reducing agents, 5% of activity remains
135509
65 - 70
-
90% denatured after 1 h
135503, 135505
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
more stable at higher protein concentrations
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0 to -20°C, 0.1 mg/ml protein concentration, 30 days, 20% and 60% loss of activity
-
stable at -20°C
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
16000 fold to homogeneity
-
16000 fold to homogeneity; high affinity for DNA
-
high affinity for DNA
-
partial, no precipitation with nucleic acids
-
recombinant protein to homogeneity
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE