Any feedback?
Information on EC 3.1.4.44 - glycerophosphoinositol glycerophosphodiesterase
for references in articles please use BRENDA:EC3.1.4.44Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.1.4.44 glycerophosphoinositol glycerophosphodiesteraseSpecify your search results
Word Map
- 3.1.4.44
- glycerophosphocholine
- dicer
-
au-rich
-
mir15a
-
let-7a
-
forensically
-
fluid-specific
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
Synonyms
mir16, gde1/mir16, glycerophosphodiester phosphodiesterase domain containing 5, glycerophosphoinositol phosphodiesterase, gpi-pde, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
GDE1
glycerophosphodiester phosphodiesterase domain containing 5
GDPD5, BC024955
glycerophosphoinositol glycerophosphohydrolase
-
-
-
-
phosphodiesterase, glycerophosphoinositol
-
-
-
-
sn-glycero(3)phosphoinositol glycerophosphohydrolase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1-(sn-glycero-3-phospho)-1D-myo-inositol + H2O = myo-inositol + sn-glycerol 3-phosphate
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
hydrolysis of phosphoric ester
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
1-(sn-glycero-3-phospho)-1D-myo-inositol glycerophosphohydrolase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
72414-13-2
not distinguished from EC 3.1.4.43
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1-(sn-glycero-3-phospho)-1D-myo-inositol + H2O
myo-inositol + sn-glycerol 3-phosphate
-
selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine
-
?
glycerophosphocholine + H2O
choline + glycerolphosphate
-
selectively hydrolyzes glycerophosphoinositol over glycerophosphocholine
-
?
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
-
enzyme selectively hydrolyzes glycerophosphoinositol
-
-
?
sn-glycero-3-phospho-1-D-myo-inositol + H2O
sn-glycero 3-phosphate + inositol
-
-
-
?
sn-glycero-3-phospho-1-D-myo-inositol + H2O
sn-glycero 3-phosphate + inositol
-
-
-
?
additional information
?
-
-
the enzyme might cleave teichoic acid and lipoteichoic acid at C1 and C3 of their glycerophosphates, respectively
-
-
?
additional information
?
-
-
glycerophosphoinositol 4-phosphate and glycerophosphoinositol 4,5-diphosphate are potential substrates
-
?
additional information
?
-
-
regulation of GDE1 activity by G protein signalling, GDE1 provides a link between phosphoinositide metabolism and G protein signal transduction, GDE1 is most likely involved in the remodeling of membrane phosphoinositides and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
regulation of GDE1 activity by G protein signalling, GDE1 provides a link between phosphoinositide metabolism and G protein signal transduction, GDE1 is most likely involved in the remodeling of membrane phosphoinositides and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking
-
?
glycerophosphoinositol + H2O
glycerol 3-phosphate + inositol
-
enzyme selectively hydrolyzes glycerophosphoinositol
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
KCl
in vitro: no direct decrease in GDPD5 activity, measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine
Mg2+
NaCl
in vivo: activity decreases (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no direct decrease in GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high NaCl concentration.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
glycerophosphoethanolamine
-
blocks the hydrolysis of glycerophosphoinositol by 30%
glycerophosphoinositol 4,5-diphosphate
-
blocks the hydrolysis of glycerophosphoinositol by 70%
Glycerophosphoinositol 4-phosphate
-
blocks the hydrolysis of glycerophosphoinositol by 30%
glycerophosphoserine
-
blocks the hydrolysis of glycerophosphoinositol by 80%
Urea
in vivo: activity decreases in vivo (mIMCD-3 cell), measuring choline by chemiluminescence, substrate sn-glycero-3-phosphocholine. in vitro: no directl inhibition of GDPD5 activity, GPC-PDE in vitro activity is reduced by over 60% in immuno-precipitates from HEK293 cells exposed to high urea concentration.
additional information
-
GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, phenylephrine reduces the GDE1 activity by 30% and lysophatidic acid by 40% compared with untreated cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
GDE1 activity expressed in HEK 293T cells can be regulated by stimulation of G protein-coupled, alpha/beta-adrenergic and lysophospholipid receptors, isoproterenol increases the GDE1 activity by 50% compared with untreated cells
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Breast Neoplasms
Glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) expression correlates with malignant choline phospholipid metabolite profiles in human breast cancer.
Diabetes Mellitus, Type 1
miR15a and miR16 in Chilean type 1 diabetes patients: possible association with apoptosis, inflammatory, or autoimmunity markers.
Echinococcosis
MicroRNA profile in immune response of alveolar and cystic echinococcosis patients.
Foot-and-Mouth Disease
Autophagic degradation of SQSTM1 inhibits ovarian cancer motility by decreasing DICER1 and AGO2 to induce MIRLET7A-3P.
Glioblastoma
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Hepatitis C
The potential regulatory role of miR16 to the interplay between interferon and transforming growth factor beta pathways through IRF3 and SMAD7 in hepatitis C virus infected patients.
Infections
The potential regulatory role of miR16 to the interplay between interferon and transforming growth factor beta pathways through IRF3 and SMAD7 in hepatitis C virus infected patients.
Leukemia, Lymphocytic, Chronic, B-Cell
Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia.
Lymphoma
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Neoplasm Metastasis
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Neoplasm Metastasis
GDPD5, a target of miR-195-5p, is associated with metastasis and chemoresistance in colorectal cancer.
Neoplasms
Differential expression of miR16 in glioblastoma and glioblastoma stem cells: their correlation with proliferation, differentiation, metastasis and prognosis.
Neoplasms
Glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) expression correlates with malignant choline phospholipid metabolite profiles in human breast cancer.
Neoplasms
In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model.
Neoplasms
Methyl(R217)HuR and MCM6 are inversely correlated and are prognostic markers in non small cell lung carcinoma.
Ovarian Neoplasms
Oleuropein reduces cisplatin resistance in ovarian cancer by targeting apoptotic pathway regulators.
Pulmonary Disease, Chronic Obstructive
A systematic review of smoking-related epigenetic alterations.
Stomach Neoplasms
In vivo multimodality imaging of miRNA-16 iron nanoparticle reversing drug resistance to chemotherapy in a mouse gastric cancer model.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
integral membrane protein, the catalytic GDE domain faces the lumen/extracellular space and the C-terminus faces the cytoplasm
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GDE1_BOVIN
331
0
37653
Swiss-Prot
Secretory Pathway (Reliability: 1)
GDE1_HUMAN
331
1
37718
Swiss-Prot
Secretory Pathway (Reliability: 1)
GDE1_MOUSE
331
0
37629
Swiss-Prot
Secretory Pathway (Reliability: 1)
GDE1_RAT
331
0
37634
Swiss-Prot
Secretory Pathway (Reliability: 1)
Q7ZUV3_DANRE
333
1
37342
TrEMBL
Secretory Pathway (Reliability: 1)
A0A812DJB8_SEPPH
282
1
32050
TrEMBL
Secretory Pathway (Reliability: 1)
A0A7R8H636_LEPSM
317
1
35819
TrEMBL
other Location (Reliability: 5)
Q28HT8_XENTR
310
0
35097
TrEMBL
Secretory Pathway (Reliability: 1)
A0A812DKP0_SEPPH
320
1
36471
TrEMBL
Secretory Pathway (Reliability: 1)
A0A0H3EAL4_BIFBP
Bifidobacterium bifidum (strain PRL2010)
353
1
38978
TrEMBL
-
Q5PQA1_XENLA
328
1
37298
TrEMBL
Secretory Pathway (Reliability: 1)
G3ICV9_CRIGR
331
0
37648
TrEMBL
Secretory Pathway (Reliability: 1)
A0A812DEI7_SEPPH
329
1
37652
TrEMBL
Secretory Pathway (Reliability: 1)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
several potential phosphorylation sites detected, possible regulation by kinase phosphorylation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in mIMCD-3 cell: GDPD5 expression reduces (mRNA level) when osmolarity is increased from 300 to 700 mosmol/kg by adding NaCl (200 mosmol/kg) and urea (200 mosmol/kg) in combination. HEK293 cells transfected with GDPD5: recombinant GDPD5 is perinuclear. GDPD5 colocalizes with neuropathy target esterase in the ER of HEK293 cells. Expression of GDPD5 increases GPC-PDE activity (specific siRNA assay to knock down GDPD5, mIMCD-3 cells)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dawson, R.M.C.; Hemington, N.; Richards, D.E.; Irvine, R.F.
sn-Glycero(3)phosphoinositol glycerophosphohydrolase. A new phosphodiesterase in rat tissues
Biochem. J.
182
39-45
1979
Rattus norvegicus
brenda
Kusser, W.; Fiedler, F.
A novel glycerophosphodiesterase from Bacillus pumilus
FEBS Lett.
166
301-306
1984
Bacillus pumilus
brenda
Zheng, B.; Berrie, C.P.; Corda, D.; Farquhar, M.G.
GDE1/MIR16 is a glycerophosphoinositol phosphodiesterase regulated by stimulation of G protein-coupled receptors
Proc. Natl. Acad. Sci. USA
100
1745-1750
2003
Rattus norvegicus
brenda
Yanaka, N.; Nogusa, Y.; Fujioka, Y.; Yamashita, Y.; Kato, N.
Involvement of membrane protein GDE2 in retinoic acid-induced neurite formation in Neuro2A cells
FEBS Lett.
581
712-718
2007
Mus musculus, Homo sapiens (Q9NZC3)
brenda
Gallazzini, M.; Ferraris, J.D.; Burg, M.B.
GDPD5 is a glycerophosphocholine phosphodiesterase that osmotically regulates the osmoprotective organic osmolyte GPC
Proc. Natl. Acad. Sci. USA
105
11026-11031
2008
Mus musculus (Q640M6)
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 3.1.4.44)
html completed
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
About BRENDA
Social media
Help
Survey
Download
Contribution
Legal
Curated at
Member of
