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Information on EC 3.1.4.4 - phospholipase D and Organism(s) Brassica oleracea var. capitata and UniProt Accession O82549

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.4 phospholipase D
IUBMB Comments
Also acts on other phosphatidyl esters.
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This record set is specific for:
Brassica oleracea var. capitata
UNIPROT: O82549
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Word Map
The taxonomic range for the selected organisms is: Brassica oleracea var. capitata
The enzyme appears in selected viruses and cellular organisms
Synonyms
pld, phospholipase d, nape-pld, phospholipase d1, dermonecrotic toxin, phospholipase d2, pc-pld, pldalpha, rpld1, spo14, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AtPLDalpha1
-
-
-
-
AtPLDalpha2
-
-
-
-
AtPLDbeta1
-
-
-
-
AtPLDbeta2
-
-
-
-
AtPLDdelta
-
-
-
-
AtPLDepsilon
-
-
-
-
AtPLDgamma1
-
-
-
-
AtPLDgamma2
-
-
-
-
AtPLDgamma3
-
-
-
-
AtPLDp1
-
-
-
-
AtPLDp2
-
-
-
-
AtPLDzeta
-
-
-
-
choline phosphatase
-
-
-
-
hPLD1
-
-
-
-
hPLD2
-
-
-
-
lecithinase D
-
-
-
-
lipophosphodiesterase II
-
-
-
-
Meiosis-specific sporulation protein SPO14
-
-
-
-
mPLD1
-
-
-
-
mPLD2
-
-
-
-
Phosphatidylcholine-hydrolyzing phospholipase D1
-
-
-
-
Phosphatidylcholine-hydrolyzing phospholipase D2
-
-
-
-
Phospholipase D1 PHOX and PX containing domain
-
-
-
-
Phospholipase D2 PHOX and PX containing domain
-
-
-
-
PLD
-
-
-
-
PLD delta
-
-
-
-
PLD epsilon
-
-
-
-
PLD zeta
-
-
-
-
PLD1C
-
-
-
-
PLDalpha
-
-
-
-
PLDalpha3
-
-
-
-
PLDbeta
-
-
-
-
PLDdelta1
-
-
-
-
PLDzeta1
-
-
-
-
PLDzeta2
-
-
-
-
rPLD1
-
-
-
-
rPLD2
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphatidylcholine phosphatidohydrolase
Also acts on other phosphatidyl esters.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-87-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphatidylcholine + ethanolamine
phosphatidylethanolamine + choline
show the reaction diagram
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
-
-
?
phosphatidylcholine + glycerol
phosphatidylglycerol + choline
show the reaction diagram
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
-
-
?
phosphatidylcholine + H2O
phosphatidate + choline
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylcholine + L-serine
phosphatidylserine + choline
show the reaction diagram
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-Ser
-
-
?
phosphatidylethanolamine + H2O
phosphatidate + ethanolamine
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylglycerol + H2O
phosphatidate + glycerol
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylinositol + H2O
phosphatidate + inositol
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylserine + H2O
phosphatidate + serine
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylcholine higher than phosphatidylglycerol equal to phosphatidylethanolamine higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
lysophosphatidylcholine + glycerol
?
show the reaction diagram
-
-
-
?
lysophosphatidylcholine + H2O
choline + lysophosphatidate
show the reaction diagram
-
about 20 times slower than reaction of phosphatidylcholine
-
?
phosphatidylcholine + D-serine
phosphatidylserine + choline
show the reaction diagram
for L- and D-serine a stereoselectivity of PLD is observed
-
-
?
phosphatidylcholine + ethanolamine
phosphatidylethanolamine + choline
show the reaction diagram
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
-
-
?
phosphatidylcholine + glycerol
?
show the reaction diagram
-
-
-
?
phosphatidylcholine + glycerol
phosphatidylglycerol + choline
show the reaction diagram
preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
-
-
?
phosphatidylcholine + H2O
choline + phosphatidate
show the reaction diagram
-
-
-
?
phosphatidylcholine + H2O
phosphatidate + choline
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylcholine + L-serine
phosphatidylserine + choline
show the reaction diagram
for L- and D-serine a stereoselectivity of PLD is observed, preference of acceptor alcohols in transphosphatidylation of phosphatidylcholine: ethanolamine higher than glycerol higher than L-serine
-
-
?
phosphatidylethanolamine + H2O
phosphatidate + ethanolamine
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylglycerol + H2O
phosphatidate + glycerol
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylglycerol equal to phosphatidylcholine higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylinositol + H2O
phosphatidate + inositol
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
phosphatidylserine + H2O
phosphatidate + serine
show the reaction diagram
the order of substrates in the rates of hydrolysis is: phosphatidylethanolamine higher than phosphatidylcholine higher than phosphatidylglycerol higher than phosphatidylinositol higher than phosphatidylserine
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CTAB
-
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
SDS
-
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
Triton X-100
-
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
388
glycerol
-
transphosphatidylation
1 - 3
lysophosphatidylcholine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.21
initial rate of transphosphatidylation, acceptor alcohol: D-Ser
1.88
initial rate of transphosphatidylation, acceptor alcohol: L-Ser
6.3
initial rate of hydrolysis, acceptor alcohol: L-Ser
7.82
initial rate of hydrolysis, acceptor alcohol: D-Ser
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PLDA1_BRAOC
810
0
91838
Swiss-Prot
other Location (Reliability: 3)
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Glycerol
-
for measurement of transfer reaction, 2 M included in media to suppress hydrolytic reaction
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography
purification by Ca2+-mediated hydrophobic interaction chromatography and an anion exchange chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Virto, C.; Svensson, I.; Adlercreutz, P.
Hydrolytic and transphosphatidylation activities of phospholipase D from Savoy cabbage towards lysophosphatidylcholine
Chem. Phys. Lipids
106
41-51
2000
Brassica oleracea var. capitata
Manually annotated by BRENDA team
Dippe, M.; Ulbrich-Hofmann, R.
Substrate specificity in phospholipid transformations by plant phospholipase D isoenzymes
Phytochemistry
70
361-365
2009
Brassica oleracea var. capitata (O82549), Brassica oleracea var. capitata (P55939), Brassica oleracea var. capitata, Papaver somniferum (Q8VWE9), Papaver somniferum (Q8W1B2), Papaver somniferum
Manually annotated by BRENDA team