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Information on EC 3.1.4.35 - 3',5'-cyclic-GMP phosphodiesterase and Organism(s) Gallus gallus and UniProt Accession P52731

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.35 3',5'-cyclic-GMP phosphodiesterase
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This record set is specific for:
Gallus gallus
UNIPROT: P52731 not found.
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Word Map
The taxonomic range for the selected organisms is: Gallus gallus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
phosphodiesterase type 5, phosphodiesterase-5, pde-5, phosphodiesterase 5, cgmp phosphodiesterase, pde5a, cyclic gmp phosphodiesterase, phosphodiesterase type-5, pde6b, cgmp-phosphodiesterase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphodiesterase 6C
-
3',5'-cyclic GMP phosphodiesterase
-
-
-
-
cGMP phosphodiesterase
-
-
-
-
cGMP-PDE
-
-
-
-
cGMP-specific phosphodiesterase
-
-
cyclic 3',5'-GMP phosphodiesterase
-
-
-
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cyclic GMP phosphodiesterase
-
-
-
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cyclic guanosine 3',5'-monophosphate phosphodiesterase
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-
-
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cyclic guanosine 3',5'-phosphate phosphodiesterase
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-
-
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guanosine cyclic 3',5'-phosphate phosphodiesterase
-
-
-
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PDE5
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splice variants PDE5A1, PDE5A2 and PDE5A3
phosphodiesterase cGMP
-
-
-
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phosphodiesterase, guanosine cyclic 3',5'-phosphate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
3',5'-cyclic-GMP 5'-nucleotidohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9068-52-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3',5'-cAMP + H2O
5'-AMP
show the reaction diagram
very low activity
-
-
?
3',5'-cGMP + H2O
5'-GMP
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.717 - 0.82
3',5'-cAMP
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
PDE5A1 and PDE5A3 show an increase from embryonic day 10 to embryonicday18 and from embryonic day 18 to 5-day post-hatching chick. PDE5A2 undergoes a marked increase (about 38fold) in the first period and remains nearly constant between embryonic day 18 and 5-day post-hatching chicken
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PDE6C_CHICK
862
0
100009
Swiss-Prot
other Location (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
full-length PDE6C, X-ray crystallography
monomer
PDE6C GAF A domain is monomeric and does not contain sufficient structural determinants to form a homodimer as found in full-length PDE6C, X-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
GAF A domain of PDE6C, sitting drop vapour diffusion method, using 1.5-1.8 M ammonium sulfate, 0.1 M sodium acetate, pH 4.5-5.0
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D166A
mutation reduces cGMP affinity
D169A
mutant enzyme shows no binding of cGMP
E124A
mutation reduces cGMP affinity
F123A
mutant enzyme shows no binding of cGMP
F123W
mutant enzyme shows no binding of cGMP
F123Y
mutation reduces cGMP affinity
K170A
mutation reduces cGMP affinity
S165A
mutation reduces cGMP affinity
S97A
mutation reduces cGMP affinity
T172A
mutant enzyme shows no binding of cGMP
T175A
mutation reduces cGMP affinity
T176A
mutant enzyme shows no binding of cGMP
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
epoxy-Sepharose cGMP affinity resin column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Huang, D.; Hinds, T.R.; Martinez, S.E.; Doneanu, C.; Beavo, J.A.
Molecular determinants of cGMP binding to chicken cone photoreceptor phosphodiesterase
J. Biol. Chem.
279
48143-48151
2004
Gallus gallus (P52731)
Manually annotated by BRENDA team
Giordano, D.; Giorgi, M.; Tata, A.M.; Modica, A.; Augusti-Tocco, G.
Expression of PDE5 splice variants during ontogenesis of chick dorsal root ganglia
J. Neurosci. Res.
78
815-823
2004
Gallus gallus
Manually annotated by BRENDA team
Martinez, S.E.; Heikaus, C.C.; Klevit, R.E.; Beavo, J.A.
The structure of the GAF A domain from phosphodiesterase 6C reveals determinants of cGMP binding, a conserved binding surface, and a large cGMP-dependent conformational change
J. Biol. Chem.
283
25913-25919
2008
Gallus gallus (P52731), Gallus gallus
Manually annotated by BRENDA team