Information on EC 3.1.4.14 - [acyl-carrier-protein] phosphodiesterase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY
3.1.4.14
-
RECOMMENDED NAME
GeneOntology No.
[acyl-carrier-protein] phosphodiesterase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
holo-[acyl-carrier protein] + H2O = 4'-phosphopantetheine + apo-[acyl-carrier protein]
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
acyl carrier protein metabolism I
-
Pantothenate and CoA biosynthesis
-
SYSTEMATIC NAME
IUBMB Comments
holo-[acyl-carrier protein] 4'-pantetheine-phosphohydrolase
The enzyme cleaves acyl-[acyl-carrier-protein] species with acyl chains of 6-16 carbon atoms although it appears to demonstrate a preference for the unacylated acyl-carrier protein (ACP) and short-chain ACPs over the medium- and long-chain species [3]. Deletion of the gene encoding this enzyme abolishes ACP prosthetic-group turnover in vivo [3]. Activation of apo-ACP to form the holoenzyme is carried out by EC 2.7.8.7, holo-[acyl-carrier-protein] synthase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4'-phosphopantetheine hydrolase
-
-
ACP hydrolase
-
-
ACP hydrolyase
P21515
-
ACP phosphodiesterase
-
-
AcpH
P21515
-
AcpH
Pseudomonas aeruginosa PAO-1
-
-
-
acyl carrier protein phosphodiesterase
-
-
acyl carrier protein phosphodiesterase
P21515
-
acyl carrier protein phosphodiesterase
-
-
acyl carrier protein phosphodiesterase
Pseudomonas aeruginosa PAO-1
-
-
-
holofatty acid synthetase hydrolase
-
-
CAS REGISTRY NUMBER
COMMENTARY
37288-21-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain PAO-1
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PAO-1
strain PAO-1
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-cAMP + H2O
3'-AMP
show the reaction diagram
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO-1
-
-
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
-
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
-
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
CoA donates 4'-phosphopantetheine as prosthetic group
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
CoA donates 4'-phosphopantetheine as prosthetic group
-
-
-
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
ACP phosphodiesterase activity is not essential for growth, although it is required for turnover of the acyl carrier protein prosthetic group in vivo, cleavage of acyl carrier protein species with acyl chains of 6-16 carbon atoms and is active on some non-native acyl carrier protein species. Preference for inacylated acyl carrier proteins and short chain acyl carrier proteins over the medium and long chain species
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO-1
-
acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation
-
-
?
acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O
?
show the reaction diagram
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO-1
-
partial conversion
-
-
?
acyl-carrier protein from Escherichia coli fatty acid synthesis pathway + H2O
?
show the reaction diagram
-
complete conversion
-
-
?
acyl-carrier-protein + H2O
4'-phosphopantetheine + apo-[acyl-carrier-protein]
show the reaction diagram
-, P21515
-
-
-
?
bis-4-nitrophenyl phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
CalE8 + H2O
?
show the reaction diagram
-
-
-
-
?
Clostridium butyricum acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein acyl-carrier protein
show the reaction diagram
-
-
-
-
?
E. coli acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein of acyl-carrier protein
show the reaction diagram
-
-
-
-
?
E. coli acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein of acyl-carrier protein
show the reaction diagram
-
no activity on large peptides of E. coli acyl-carrier-protein
-
-
?
ethyldethia-acyl-carrier protein + H2O
? + apoprotein
show the reaction diagram
-
-
-
-
?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
show the reaction diagram
-
-
-
-
?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
show the reaction diagram
-
no activity on coenzyme A, 1 mol holo-fatty acid synthetase contains 1 mol 4'-phosphopantetheine
-
-
?
peptidyl carrier protein domain of non-ribosomal peptide synthase + H2O
?
show the reaction diagram
-
complete conversion
-
-
?
thymidine 5-monophosphate 4-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
show the reaction diagram
-
specific for rat liver holo-fatty acid synthetase, no activity on pigeon liver holo-fatty acid synthetase
-
-
?
additional information
?
-
-
AcpH does not hydrolyze 3',5'-cAMP
-
-
-
additional information
?
-
-
AcpH readily removes the N-pentylpantothenamide-modified prosthetic group of acyl carrier proteinboth in vivo and in vitro
-
-
-
additional information
?
-
-
acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH
-
-
-
additional information
?
-
Pseudomonas aeruginosa PAO-1
-
AcpH does not hydrolyze 3',5'-cAMP, acyl-carrier protein domain from the modular polyketide synthase DEBS is a poor substrate for AcpH
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
-
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
-
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
CoA donates 4'-phosphopantetheine as prosthetic group
-
-
?
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
CoA donates 4'-phosphopantetheine as prosthetic group
-
-
-
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
-
ACP phosphodiesterase activity is not essential for growth, although it is required for turnover of the acyl carrier protein prosthetic group in vivo
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
-
required, or other divalent cations
Fe2+
-
required, or other divalent cations
Mg2+
-
required, or other divalent cations
Mg2+
-
required, or other divalent cations
Mn2+
-
required, or other divalent cations
Mn2+
-
increased activity in combination with Mg2+
Mn2+
-, P21515
required
Zn2+
-
required, or other divalent cations
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
required
2-mercaptoethanol
-
required
dithiothreitol
-
required
dithiothreitol
-
required
Triton X-100
-
biphasic, optimal stimulation at 1 mM
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0017
-
acyl-carrier protein
-
-
0.4
-
bis-4-nitrophenyl phosphate
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.004
-
2',3'-cAMP
-
-
0.21
-
bis-4-nitrophenyl phosphate
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.5
10.5
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.2
-
-
not active below
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
aggregates upon overexpression in vivo or concentration in vitro
-
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 25000, SDS-PAGE
monomer
-
about half of the soluble fraction of AcpH is obtained as monomeric protein
monomer
Pseudomonas aeruginosa PAO-1
-
about half of the soluble fraction of AcpH is obtained as monomeric protein
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
60
-
-
loss of activity after 15 min in the absence of SDS
90
-
-
stable in the presence of 1% SDS
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
90°C, 1% SDS, 10 min, 20% loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
AcpH is stable enough that it can be concentrated and stored at concentrations up to 1 mg/ml without causing severe aggregation
-
-20oC, dithiothreitol, several months, no loss of activity
-
-20oC, under nitrogen, dithiothreitol or 2-mercaptoethanol, long periods, no loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Ni2+-NTA resin column chromatography and Superdex-75 gel filtration
-
1 mol holo-fatty acid synthetase contains 1 mol 4'-phosphopantetheine
-
1 mol holo-fatty acid synthetase contains 1 mol 4'-phosphopantetheine; partial
-
4'-phosphopantetheine is periodically removed from and replaced on the holo-fatty acid synthetase
-
partial, amino acid composition
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D24N
-, P21515
low level of activity
D78N
-, P21515
no activity
D82N
-, P21515
no activity
H6Q
-, P21515
low level of activity
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
refolding in purification step using Profoldin soluble protein folding column
-, P21515