The enzyme cleaves acyl-[acyl-carrier-protein] species with acyl chains of 6-16 carbon atoms although it appears to demonstrate a preference for the unacylated acyl-carrier protein (ACP) and short-chain ACPs over the medium- and long-chain species . Deletion of the gene encoding this enzyme abolishes ACP prosthetic-group turnover in vivo . Activation of apo-ACP to form the holoenzyme is carried out by EC 126.96.36.199, holo-[acyl-carrier-protein] synthase.
ACP phosphodiesterase activity is not essential for growth, although it is required for turnover of the acyl carrier protein prosthetic group in vivo, cleavage of acyl carrier protein species with acyl chains of 6-16 carbon atoms and is active on some non-native acyl carrier protein species. Preference for inacylated acyl carrier proteins and short chain acyl carrier proteins over the medium and long chain species
acyl-carrier protein holo-PA2966 is converted completely back to its apo-form within 40 min, whereas the acyl-carrier proteins PA1869 and PA4226 remain in its holo form even after prolonged incubation. Partial conversion is observed for acyl-carrier protein PA3334 with both the apo and holo forms present after the incubation