Information on EC 3.1.4.14 - [acyl-carrier-protein] phosphodiesterase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.4.14
-
RECOMMENDED NAME
GeneOntology No.
[acyl-carrier-protein] phosphodiesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
holo-[acyl-carrier protein] + H2O = 4'-phosphopantetheine + apo-[acyl-carrier protein]
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acyl carrier protein metabolism
-
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lipid metabolism
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Pantothenate and CoA biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
holo-[acyl-carrier protein] 4'-pantetheine-phosphohydrolase
The enzyme cleaves acyl-[acyl-carrier-protein] species with acyl chains of 6-16 carbon atoms although it appears to demonstrate a preference for the unacylated acyl-carrier protein (ACP) and short-chain ACPs over the medium- and long-chain species [3]. Deletion of the gene encoding this enzyme abolishes ACP prosthetic-group turnover in vivo [3]. Activation of apo-ACP to form the holoenzyme is carried out by EC 2.7.8.7, holo-[acyl-carrier-protein] synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-21-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PAO-1
-
-
Manually annotated by BRENDA team
strain PAO-1
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-cAMP + H2O
3'-AMP
show the reaction diagram
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
acyl-carrier protein domain of the iterative polyketide synthase in calicheamicin biosynthesis + H2O
?
show the reaction diagram
acyl-carrier protein from Escherichia coli fatty acid synthesis pathway + H2O
?
show the reaction diagram
-
complete conversion
-
-
?
acyl-carrier-protein + H2O
4'-phosphopantetheine + apo-[acyl-carrier-protein]
show the reaction diagram
-
-
-
?
bis-4-nitrophenyl phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
CalE8 + H2O
?
show the reaction diagram
-
-
-
-
?
Clostridium butyricum acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein acyl-carrier protein
show the reaction diagram
-
-
-
-
?
E. coli acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein of acyl-carrier protein
show the reaction diagram
ethyldethia-acyl-carrier protein + H2O
? + apoprotein
show the reaction diagram
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-
-
-
?
holo-fatty acid synthetase + H2O
apo-fatty acid synthetase + 4'-phosphopantetheine
show the reaction diagram
peptidyl carrier protein domain of non-ribosomal peptide synthase + H2O
?
show the reaction diagram
-
complete conversion
-
-
?
thymidine 5-monophosphate 4-nitrophenyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-carrier protein + H2O
4'-phosphopantetheine + apoprotein
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
required, or other divalent cations
Fe2+
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required, or other divalent cations
Zn2+
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required, or other divalent cations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
dithiothreitol
Triton X-100
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biphasic, optimal stimulation at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
2',3'-cAMP
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0.0017
acyl-carrier protein
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0.4
bis-4-nitrophenyl phosphate
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
2',3'-cAMP
Pseudomonas aeruginosa
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0.21
bis-4-nitrophenyl phosphate
Pseudomonas aeruginosa
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 10.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
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not active below
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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aggregates upon overexpression in vivo or concentration in vitro
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Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 25000, SDS-PAGE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
loss of activity after 15 min in the absence of SDS
90
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stable in the presence of 1% SDS
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
90°C, 1% SDS, 10 min, 20% loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20oC, dithiothreitol, several months, no loss of activity
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-20oC, under nitrogen, dithiothreitol or 2-mercaptoethanol, long periods, no loss of activity
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AcpH is stable enough that it can be concentrated and stored at concentrations up to 1 mg/ml without causing severe aggregation
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
1 mol holo-fatty acid synthetase contains 1 mol 4'-phosphopantetheine
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1 mol holo-fatty acid synthetase contains 1 mol 4'-phosphopantetheine; partial
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4'-phosphopantetheine is periodically removed from and replaced on the holo-fatty acid synthetase
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Ni2+-NTA resin column chromatography and Superdex-75 gel filtration
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partial
partial, amino acid composition
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D24N
low level of activity
D78N
no activity
D82N
no activity
H6Q
low level of activity
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
refolding in purification step using Profoldin soluble protein folding column
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