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The taxonomic range for the selected organisms is: Streptomyces griseocarneus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
sphingomyelinase, alpha-toxin, acid sphingomyelinase, smase, neutral sphingomyelinase, asmase, nsmase2, nsmase, smpd1, n-smase,
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acid sphingomyelinase
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alkaline sphingomyelinase
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neutral sphingomyelinase
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phosphodiesterase, sphingomyelin
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sphingomyelin phosphodiesterase
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sphingomyelinase C
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hydrolysis of phosphoric ester
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sphingomyelin cholinephosphohydrolase
Has very little activity on phosphatidylcholine.
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1,2-di [(cis)-9-octadecenoyl]-sn-glycero-3-phospho-(1-rac-glycerol) + H2O
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2.11% activity compared to sphingomyelin
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1,2-di(cis-9-octadecenoyl)-sn-glycerol-3-phosphate + H2O
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9.04% activity compared to sphingomyelin
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1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O
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4.93% activity compared to sphingomyelin
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1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O
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0.7% activity compared to sphingomyelin
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L-alpha-phosphatidylinositol + H2O
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5.63% activity compared to sphingomyelin
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sphingomyelin + H2O
N-acylsphingosine + choline phosphate
100% activity
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additional information
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additional information
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no activity towards 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
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additional information
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no activity towards 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
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Cu2+
107% activity at 1 mM Mg2+
Fe2+
104% activity at 1 mM Mg2+
Mg2+
required, 100% activity at 2.5-10 mM Mg2+
Mn2+
required, 100% activity at 1 mM Mg2+
additional information
not stimulated by Ca2+, CO2+, and Fe3+
additional information
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not stimulated by Ca2+, CO2+, and Fe3+
additional information
the order of potency to enhance the activity is Zn2+>Mn2+>Cu2+>Fe2+
additional information
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the order of potency to enhance the activity is Zn2+>Mn2+>Cu2+>Fe2+
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dithiothreitol
33.1% residual activity at 2 mM
EDTA
complete inhibition at 2 mM
phenylmethylsulfonyl fluoride
70.3% residual activity at 2 mM
SDS
67.7% residual activity at 2 mM
additional information
the enzyme is not inhibited by mercaptoethanol and iodoacetamide
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additional information
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the enzyme is not inhibited by mercaptoethanol and iodoacetamide
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sodium cholate
129% relative activity at 6% (v/v) sodium cholate
Triton X-100
205% relative activity at 0.1% (v/v) Triton X-100
additional information
not affected by 6% sodium taurocholate and 6% sodium deoxycholate
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additional information
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not affected by 6% sodium taurocholate and 6% sodium deoxycholate
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0.246
culture supernatant, in 50 mM glycine-NaOH buffer (pH 9.6), at 37°C
98.4
400fold purified enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 55°C
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UniProt
brenda
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A6P7M9_9ACTN
339
0
36221
TrEMBL
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32102
1 * 32102, MALDI-TOF mass spectrometry
32130
calculated from amino acid sequence
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monomer
1 * 32102, MALDI-TOF mass spectrometry
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5 - 10
stable from pH 5.0-10.0, the enzyme retains activity between pH 5 and 10 for 30 min
704743
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4 - 60
the enzyme activity is stable between 4°C and 37°C for 30 min, activity drops abruptly above 37°C with less than 10% activity remaining at 50-60°C
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ammonium sulfate precipitation, DEAE-Sepharose column chromatography, HiTrap Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
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expressed in Escherichia coli DH5alpha cells
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Sugimori, D.
Purification, characterization, and gene cloning of sphingomyelinase C from Streptomyces griseocarneus NBRC13471
J. Biosci. Bioeng.
108
293-298
2009
Streptomyces griseocarneus (A6P7M9), Streptomyces griseocarneus, Streptomyces griseocarneus NBRC13471 (A6P7M9)
brenda