Information on EC 3.1.4.12 - sphingomyelin phosphodiesterase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.1.4.12
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RECOMMENDED NAME
GeneOntology No.
sphingomyelin phosphodiesterase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a sphingomyelin + H2O = a ceramide + phosphocholine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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additional information
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SMase belongs to the family of interfacial enzymes that carry out processive catalytic turnover at the interface, SMase binds rapidly and avidly to sphingomyelin vesicles and it is fully active as a monomer at the interface
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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sphingolipid biosynthesis (mammals)
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Sphingolipid metabolism
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sphingomyelin metabolism
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SYSTEMATIC NAME
IUBMB Comments
sphingomyelin cholinephosphohydrolase
Has very little activity on phosphatidylcholine.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-54-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
nSMase
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
nSMase
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Manually annotated by BRENDA team
serovar Lai, gene sph2 or LA_1029 encoding isozyme Sph2
UniProt
Manually annotated by BRENDA team
serovar Lai, gene sph2 or LA_1029 encoding isozyme Sph2
UniProt
Manually annotated by BRENDA team
nSMase
EMBL
Manually annotated by BRENDA team
recombinant
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Manually annotated by BRENDA team
Mus musculus C57BL/6J
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Manually annotated by BRENDA team
strain Honduras 1, no. 1-CDC
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Manually annotated by BRENDA team
Plasmodium falciparum Honduras 1
strain Honduras 1, no. 1-CDC
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Manually annotated by BRENDA team
strain TK4
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Manually annotated by BRENDA team
Rattus norvegicus Fischer 344
strain Fischer 344
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Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
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Manually annotated by BRENDA team
strain NBRC13471
UniProt
Manually annotated by BRENDA team
strain NBRC13471
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-di [(cis)-9-octadecenoyl]-sn-glycero-3-phospho-(1-rac-glycerol) + H2O
?
show the reaction diagram
1,2-di(cis-9-octadecenoyl)-sn-glycerol-3-phosphate + H2O
?
show the reaction diagram
1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O
?
show the reaction diagram
1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O
?
show the reaction diagram
0.7% activity compared to sphingomyelin
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?
1-alkyl-lyso-platelet activating factor + H2O
1-alkylglycerol + choline phosphate
show the reaction diagram
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?
1-O-octadecyl-2-lyso-platelet-activating factor + H2O
1-O-octadecyl-sn-glycerol + phosphocholine
show the reaction diagram
very little activity
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?
2-(N-hexadecanoylamino)-4-nitrophenylphosphorylcholine + H2O
2-(N-hexadecanoylamino)-4-nitrophenol + choline phosphate
show the reaction diagram
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?
2-hexadecanoylamino-4-nitrophenylphosphocholine + H2O
2-hexadecanoylamino-4-nitrophenol + choline phosphate
show the reaction diagram
2-n-hexadecanoylamino-4-nitrophenylphosphorylcholine + H2O
2-n-hexadecanoylamino-4-nitrophenol + choline phosphate
show the reaction diagram
4-nitrobenz-2-oxa-1,3-diazole-sphingosylphosphorylcholine + H2O
4-nitrobenz-2-oxa-1,3-diazole-sphingosine + choline phosphate
show the reaction diagram
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?
6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-hexanoyl)sphingosyl phosphocholine + H2O
6-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino-N-hexanoylsphingosine + choline phosphate
show the reaction diagram
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?
6-hexadecanoylamino-4-methylumbelliferyl-phosphocholine + H2O
6-hexadecanoylamino-4-methylumbelliferone + choline phosphate
show the reaction diagram
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?
6-hexadecanoylamino-4-methylumbelliferyl-phosphorylcholine + H2O
6-hexadecanoylamino-4-methylumbelliferone + choline phosphate
show the reaction diagram
a sphingomyelin + H2O
a ceramide + choline phosphate
show the reaction diagram
a sphingomyelin + H2O
a ceramide + phosphocholine
show the reaction diagram
bis(4-methylumbelliferyl)-phosphate + H2O
?
show the reaction diagram
bis-p-nitrophenyl phosphate + H2O
?
show the reaction diagram
C6-7-nitro-2-1,3-benzoxadiazol-4-yl-sphingomyelin + H2O
?
show the reaction diagram
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?
choline-methyl-sphingomyelin + H2O
?
show the reaction diagram
hexadecanoyl-p-nitrophenylphosphorylcholine + H2O
hexadecanoyl-p-nitrophenol + choline phosphate
show the reaction diagram
L-alpha-phosphatidylinositol + H2O
?
show the reaction diagram
5.63% activity compared to sphingomyelin
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?
lissamine-rhodamine dodecanoyl sphingosyl phosphocholine + H2O
?
show the reaction diagram
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neutral sphingomyelinase
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?
lyso-platelet-activating factor + H2O
1-alkylglycerol + choline phosphate
show the reaction diagram
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103fold higher activity than with sphingomyelin in the absence of detergent
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?
lyso-platelet-activating factor + H2O
alkylglycerol + choline phosphate
show the reaction diagram
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55% of activity with sphingomyelin in the absence of detergent
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?
lysophosphatidylcholine + H2O
1-acylglycerol + choline phosphate
show the reaction diagram
lysophosphatidylcholine + H2O
acylglycerol + choline phosphate
show the reaction diagram
N-dodecanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O
4-nitrobenz-2-oxa-1,3-diazole-N-dodecanoylsphingosine + choline phosphate
show the reaction diagram
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?
N-hexanoyl-4-nitrobenz-2-oxa-1,3-diazole-sphingomyelin + H2O
4-nitrobenz-2-oxa-1,3-diazole-N-hexanoylsphingosine + choline phosphate
show the reaction diagram
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?
N-methyl-sphingomyelin + H2O
N-methyl-N-acylsphingosine + choline phosphate
show the reaction diagram
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?
N-methylsphingomyelin + H2O
?
show the reaction diagram
p-nitrophenylphosphorylcholine + H2O
choline phosphate + p-nitrophenol
show the reaction diagram
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?
phosphatidylcholine + H2O
diacylglycerol + choline phosphate
show the reaction diagram
phosphatidylethanolamine + H2O
diacylglycerol + ethanolamine phosphate
show the reaction diagram
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low activity
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?
phosphatidylethanolamine + H2O
diacylglycerol + phosphorylethanolamine
show the reaction diagram
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in the presence of 0.08% sodium taurodeoxycholate
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?
phosphatidylglycerol + H2O
diacylglycerol + glycerolphosphate
show the reaction diagram
platelet-activating factor + H2O
1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
show the reaction diagram
platelet-inactivating factor + H2O
1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
show the reaction diagram
sphingomyelin + H2O
?
show the reaction diagram
sphingomyelin + H2O
N-acylsphingosine + choline phosphate
show the reaction diagram
sphingomyelin + H2O
N-acylsphingosine + choline phosphate + ?
show the reaction diagram
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?
sphingosylphosphocholine + H2O
sphingosine + choline phosphate
show the reaction diagram
sphingosylphosphorylcholine + H2O
sphingosine + choline phosphate
show the reaction diagram
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25% of activity with sphingomyelin
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a sphingomyelin + H2O
a ceramide + choline phosphate
show the reaction diagram
a sphingomyelin + H2O
a ceramide + phosphocholine
show the reaction diagram
phosphatidylethanolamine + H2O
diacylglycerol + ethanolamine phosphate
show the reaction diagram
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low activity
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?
platelet-activating factor + H2O
1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
show the reaction diagram
platelet-inactivating factor + H2O
1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
show the reaction diagram
sphingomyelin + H2O
?
show the reaction diagram
sphingomyelin + H2O
N-acylsphingosine + choline phosphate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
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Ni2+
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additional information