Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.1.4.11 - phosphoinositide phospholipase C and Organism(s) Pisum sativum and UniProt Accession O24297

for references in articles please use BRENDA:EC3.1.4.11
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.4 Phosphoric-diester hydrolases
                3.1.4.11 phosphoinositide phospholipase C
IUBMB Comments
These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Pisum sativum
UNIPROT: O24297
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Pisum sativum
The enzyme appears in selected viruses and cellular organisms
Synonyms
pi-plc, phosphatidylinositol-specific phospholipase c, plc-gamma, plc-gamma1, plcgamma1, plc-beta, plcgamma, phosphoinositide-specific phospholipase c, plcgamma2, phospholipase c-gamma1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphatidylinositol-4,5-bisphosphate-specific phospholipase C
-
PIP2-specific phospholipase C
-
1-phosphatidyl-D-myo-inositol 4,5-bisphosphate inositoltrisphosphohydrolase
-
-
-
-
1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase
-
-
-
-
No receptor potential A protein
-
-
-
-
phosphatidylinositol 4,5-bisphosphate phosphodiesterase
-
-
-
-
phosphatidylinositol-4,5-bisphosphate phosphodiesterase
-
-
-
-
phosphatidylinositol-4,5-bisphosphate phospholipase C
-
-
-
-
phosphodiesterase, triphosphoinositide
-
-
-
-
phosphoinositidase C
-
-
-
-
phosphoinositide-specific phospholipase C
-
-
phospholipase C
-
-
-
-
phosphotidylinositol 4,5-bisphosphate-specific phospholipase C
-
-
-
-
PI-PLC
-
-
PIC
-
-
-
-
PIP(2)-specific-phospholipase
-
-
PIP2 PDE
-
-
-
-
PIP2 phosphodiesterase
-
-
-
-
PIP2-PLC
-
-
PIPLC
-
-
-
-
PLC
-
-
-
-
PLC-148
-
-
-
-
PLC-154
-
-
-
-
PLC-85
-
-
-
-
polyphosphoinositide phospholipase C
-
-
-
-
PtdIns(4,5)P2-directed phospholipase C
-
-
-
-
triphosphoinositide phosphodiesterase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate inositoltrisphosphohydrolase
These enzymes form some of the cyclic phosphate Ins(cyclic1,2)P(4,5)P2 as well as Ins(1,4,5)P3. They show activity towards phosphatidylinositol, i.e., the activity of EC 4.6.1.13, phosphatidylinositol diacylglycerol-lyase, in vitro at high [Ca2+]. Four beta-isoforms regulated by G-proteins, two gamma-forms regulated by tyrosine kinases, four delta-forms regulated at least in part by calcium and an epsilon-form, probably regulated by the oncogene ras, have been found.
CAS REGISTRY NUMBER
COMMENTARY hide
37213-51-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
?
1-phosphatidyl-1D-myo-inositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O
1D-myo-inositol 1,4-bisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol + H2O
1D-myo-inositol 1-phosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O
1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
1-phosphatidyl-1D-myo-inositol 4-phosphate + H2O
1D-myo-inositol 1,4-bisphosphate + diacylglycerol
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
coordinated at the active site
Ca2+
-
activates
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
U-73122
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
phosphatidylinositol-4,5-bisphosphate-specific phospholipase C appears to play a key role in free salicylic acid and abscisic acid-associated reinforcement of thermotolerance resulting from heat acclimation
Manually annotated by BRENDA team
higher expression of PsPLC delta isoform in roots than in shoots
Manually annotated by BRENDA team
light-grown
Manually annotated by BRENDA team
lower expression of PsPLC delta isoform in shoots than in roots
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
recombinant PLC delta isoform expressed in Escherichia coli
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
the enzyme plays a central role in regulating early events downstream of immune-receptor activation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
O24297_PEA
594
0
67436
TrEMBL
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
67440
x * 67440, PLC delta isoform, calculated from the amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 67440, PLC delta isoform, calculated from the amino acid sequence
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from inclusion bodies, inactive recombinant PLC delta isoform, may be due to an incorrect refolding
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
PLC delta isoform, expression in Escherichia coli BRL (DE3), sequencing, genomic organization
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Venkataraman, G.; Goswami, M.; Tuteja, N.; Reddy, M.K.; Sopory, S.K.
Isolation and characterization of a phospholipase C delta isoform from pea that is regulated by light in a tissue specific manner
Mol. Genet. Genomics
270
378-386
2003
Pisum sativum (O24297), Pisum sativum
Manually annotated by BRENDA team
Liu, H.T.; Huang, W.D.; Pan, Q.H.; Weng, F.H.; Zhan, J.C.; Liu, Y.; Wan, S.B.; Liu, Y.Y.
Contributions of PIP(2)-specific-phospholipase C and free salicylic acid to heat acclimation-induced thermotolerance in pea leaves
J. Plant Physiol.
163
405-416
2006
Pisum sativum
Manually annotated by BRENDA team
Liu, H.T.; Liu, Y.Y.; Pan, Q.H.; Yang, H.R.; Zhan, J.C.; Huang, W.D.
Novel interrelationship between salicylic acid, abscisic acid, and PIP2-specific phospholipase C in heat acclimation-induced thermotolerance in pea leaves
J. Exp. Bot.
57
3337-3347
2006
Pisum sativum (O24297), Pisum sativum
Manually annotated by BRENDA team
Abd-El-Haliem, A.M.; Joosten, M.H.
Plant phosphatidylinositol-specific phospholipase C at the center of plant innate immunity
J. Integr. Plant Biol.
59
164-179
2017
Arabidopsis thaliana, Brassica napus, Citrus limon, Glycine max, Medicago sativa, Solanum lycopersicum, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Rubia tinctorum, Nicotiana benthamiana, Capsicum chinense
Manually annotated by BRENDA team