Information on EC 3.1.4.1 - phosphodiesterase I

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.1.4.1
-
RECOMMENDED NAME
GeneOntology No.
phosphodiesterase I
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric esters
phosphoric ester hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
oligonucleotide 5'-nucleotidohydrolase
Hydrolyses both ribonucleotides and deoxyribonucleotides. Has low activity towards polynucleotides. A 3'-phosphate terminus on the substrate inhibits hydrolysis.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-82-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
nutritional regulation of enzyme activity
-
-
Manually annotated by BRENDA team
Aspergillus batatae
-
-
-
Manually annotated by BRENDA team
Aspergillus fuscus
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
oat
-
-
Manually annotated by BRENDA team
strain BG581
-
-
Manually annotated by BRENDA team
var. saccharifera, sugar beet
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
commercial product
-
-
Manually annotated by BRENDA team
carrot
-
-
Manually annotated by BRENDA team
extremely halophilic bacterium, mixture of 3'- and 5'-exonuclease
-
-
Manually annotated by BRENDA team
R26
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
yellow lupin
-
-
Manually annotated by BRENDA team
isozymes NPP1, Npp2, NPP3
-
-
Manually annotated by BRENDA team
Mus musculus BALB/c
BALB/c
-
-
Manually annotated by BRENDA team
Mus musculus C57/black
gene SMPDL3A
UniProt
Manually annotated by BRENDA team
tobacco
-
-
Manually annotated by BRENDA team
native and immobilized on Eupergit C enzyme
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
male
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Rhizobium leguminosarum bv. viciae strain 3841
-
-
Manually annotated by BRENDA team
bifunctional phosphodiesterase/phosphomonoesterase
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
synthetic construct
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10-hydroxydecyl-oligonucleotide + H2O
?
show the reaction diagram
-
different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site
-
-
?
2-hydroxypropyl-p-nitrophenyl phosphate + H2O
2-hydroxypropyl-p-nitrophenol + phosphate
show the reaction diagram
synthetic construct
-
-
-
?
2-naphthyl chloromethylphosphonate + H2O
2-naphthol + chloromethylphosphonic acid
show the reaction diagram
-
-
-
-
?
2-naphthyl methylphosphonate + H2O
2-naphthol + methylphosphonic acid
show the reaction diagram
-
-
-
-
?
2-naphthyl phenylphosphonate + H2O
2-naphthol + benzenephosphonic acid
show the reaction diagram
-
-
-
-
?
3'-O-acetyl-5'-O-[hydroxy(4-nitrophenoxy)phosphoryl]thymidine + H2O
p-nitrophenol + 3'-O-acetylthymidine 5'-phosphate
show the reaction diagram
-
-
-
-
?
3'-p-nitrophenylthymidine 3'-phosphate + H2O
3'-TMP + p-nitrophenol
show the reaction diagram
-
-
-
-
?
3'-phosphoadenosine 5'-phosphosulfate + H2O
?
show the reaction diagram
-
-
-
-
?
3-hydroxy-2(hydroxymethyl)-tetrahydrofuran-oligonucleotide + H2O
?
show the reaction diagram
-
different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site. In the case of tetrahydrofuran, the enzyme generates break with the 5'-tetrahydrofuran and the 3'-phosphate termini
the enzyme generates a non-phosphorylated 5'-THF-terminus by the cleavage of 3-hydroxy-2(hydroxymethyl)-tetrahydrofuran-containing DNA
-
?
4-methylumbelliferyl phenylphosphonate + H2O
4-methylumbelliferone + phenylphosphonic acid
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl thymidine 5'-phosphate + H2O
4-methylumbelliferone + 5'-TMP
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phenyl phosphonate + H2O
4-nitrophenol + phenyl phosphonate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl phosphocholine + H2O
4-nitrophenol + phosphocholine
show the reaction diagram
secreted recombinant human SMPDL3A hydrolyzes 4-nitrophenyl phosphorylcholine, a synthetic analogue of the phosphorylcholine headgroup of lipids such as sphingomyelin and phosphatidylcholine
-
-
?
4-nitrophenyl phosphorothioate + H2O
?
show the reaction diagram
-
-
-
-
?
4-nitrophenyl sulfate + H2O
?
show the reaction diagram
-
-
-
-
?
5'-p-nitrophenyl deoxythymidine 5'-phosphate + H2O
5'-dTMP + p-nitrophenol
show the reaction diagram
5'-p-nitrophenyl-2',3'-isopropyl-5'-UMP + H2O
?
show the reaction diagram
-
-
-
-
?
5'-p-nitrophenyladenosine 5'-phosphate + H2O
5'-AMP + p-nitrophenol
show the reaction diagram
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
5'-p-nitrophenylnucleoside 5'-monophosphate + H2O
nucleoside 5'-monophosphate + p-nitrophenol
show the reaction diagram
5'-p-nitrophenyluridine 5'-phosphate + H2O
5'-UMP + p-nitrophenol
show the reaction diagram
adenosine 5'-P1-tetraphospho-P4-5'''-adenosine + H2O
AMP + ATP
show the reaction diagram
adenosine 5'-P1-tetraphospho-P4-5'''-guanosine + H2O
?
show the reaction diagram
-
-
-
-
?
adenosine 5'-phosphosulfate + H2O
?
show the reaction diagram
-
-
-
-
?
adenosyl uridine 5'-phosphate + H2O
5'-UMP + adenosine
show the reaction diagram
-
-
-
?
adenylyl-2',5'-adenosine + H2O
?
show the reaction diagram
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
adenylyl-3',5'-adenosine + H2O
adenosine + 5'-AMP
show the reaction diagram
adenylyl-3',5'-cytidine + H2O
adenosine 3'-phosphate + cytidine
show the reaction diagram
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
adenylyl-3',5'-uridine + H2O
?
show the reaction diagram
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
ADP + H2O
AMP + phosphate
show the reaction diagram
ADP-ribose + H2O
5'-AMP + ribosyl monophosphate
show the reaction diagram
-
-
-
?
ADP-ribose + H2O
?
show the reaction diagram
-
-
-
?
ApA + H2O
5'-AMP + adenosine
show the reaction diagram
ApC + H2O
5'-CMP + adenosine
show the reaction diagram
-
-
-
?
ApG + H2O
5'-GMP + adenosine
show the reaction diagram
-
-
-
?
ATP + H2O
5'-AMP + diphosphate
show the reaction diagram
ATP + H2O
AMP + diphosphate
show the reaction diagram
-
-
-
-
?
bis(4-methylumbelliferyl) phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
bis(4-nitrophenyl) phosphate + H2O
4-nitrophenyl phosphate + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
bis(4-nitrophenyl)phosphate + H2O
4-nitrophenyl phosphate + 4-nitrophenol
show the reaction diagram
-
-
-
-
?
bis(p-nitrophenyl) phosphate + H2O
?
show the reaction diagram
bis-4-nitrophenyl phosphate + H2O
4-nitrophenol + 4-nitrophenyl phosphate
show the reaction diagram
-
-
-
-
?
bis-ethylene glycolyl-oligonucleotide + H2O
?
show the reaction diagram
-
different sequences of the oligonucleotides, overview. The enzyme catalyzes the cleavage of the synthetic analogue of the apurinic/apyrimidinic site
-
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + p-nitrophenyl phosphate
show the reaction diagram
bis-p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
processive two-step mechanism
-
?
bis-p-nitrophenyl phosphate + H2O
p-nitrophenyl phosphate + nitrophenol
show the reaction diagram
cAMP + H2O
?
show the reaction diagram
CDP-choline + H2O
?
show the reaction diagram
-
-
-
-
?
CpU + H2O
5'-UMP + cytidine
show the reaction diagram
-
-
-
?
cyclic 3',5'-mononucleotides + H2O
mononucleoside 5'-phosphate
show the reaction diagram
cytidylyl-3',5'-adenosine + H2O
?
show the reaction diagram
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
diadenosine 5',5'''-P1,P3-triphosphate + H2O
AMP + ADP
show the reaction diagram
diadenosine diphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
diadenosine polyphosphate + H2O
AMP
show the reaction diagram
diadenosine polyphosphate + H2O
AMP + adenosine polyphosphate-1
show the reaction diagram
-
-
-
?
diethenoadenosine polyphosphate + H2O
ethenoadenosine 5'-monophosphate + ethenoadenosine (n-1)5'-polyphosphate
show the reaction diagram
-
-
-
-
?
dinucleoside monophosphates + H2O
mononucleoside 5'-phosphate
show the reaction diagram
dinucleoside polyphosphate + H2O
?
show the reaction diagram
dinucleoside tetraphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
dinucleotides + H2O
?
show the reaction diagram
DNA + H2O
DNA(n-1) + 2'-deoxynucleoside 5'-phosphate
show the reaction diagram
downstream cleavage product-RNA + H2O
?
show the reaction diagram
-
-
-
-
?
FAD + H2O
AMP + FMN
show the reaction diagram
-
-
-
?
GDP-mannose + H2O
?
show the reaction diagram
-
-
-
-
?
GTP + H2O
?
show the reaction diagram
-
-
-
-
?
guanylyl-3',5'-adenosine + H2O
?
show the reaction diagram
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
methyl 4-nitrophenyl phosphorothioate + H2O
?
show the reaction diagram
-
-
-
-
?
NAD+ + H2O
5'-AMP + NMN
show the reaction diagram
NADP+ + H2O
NAD+ + phosphate
show the reaction diagram
oligonucleotides + H2O
oligonucleotides(n-1) + 5'-mononucleotides
show the reaction diagram
p-nitrophenyl 5'-thymidine monophosphate + H2O
5'-TMP + p-nitrophenol
show the reaction diagram
-
-
-
-
p-nitrophenyl 5'-thymidine monophosphate + H2O
p-nitrophenol + 5'-thymidine monophosphate
show the reaction diagram
p-nitrophenyl chloromethylphosphonate + H2O
p-nitrophenol + chloromethylphosphonic acid
show the reaction diagram
-
-
-
-
?
p-nitrophenyl ethyl phosphate + H2O
p-nitrophenol + ethyl hydrogen phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl methylphosphonate + H2O
p-nitrophenol + methylphosphonic acid
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + benzenephosphonic acid
show the reaction diagram
p-nitrophenyl phenylphosphonate + H2O
p-nitrophenol + phenylphosphonic acid
show the reaction diagram
-
-
-
-
?
p-nitrophenylphosphorylcholine + H2O
p-nitrophenol + phosphorylcholine
show the reaction diagram
p-nitrophenylthymidine 5'-phosphate + H2O
5'-TMP + p-nitrophenol
show the reaction diagram
P1,P4-bis(5'-uridyl) tetraphosphate + H2O
UMP + uridine 5'-triphosphate
show the reaction diagram
-
-
-
?
phosphonate ester + H2O
?
show the reaction diagram
-
-
-
-
?
poly A + H2O
5'-AMP
show the reaction diagram
poly C + H2O
5'-CMP
show the reaction diagram
poly d(A-T)2 + H2O
?
show the reaction diagram
-
-
-
-
?
poly G + H2O
5'-GMP
show the reaction diagram
-
-
-
?
poly U + H2O
5'-UMP
show the reaction diagram
poly(I) + H2O
5'-IMP
show the reaction diagram
polymer synthesized from NAD+ + H2O
?
show the reaction diagram
-
chain length 27-30
-
-
?
polynucleotides + H2O
polynucleotides(n-1) + 5'-mononucleotides
show the reaction diagram
RNA + H2O
5'-GMP + 5'-AMP + ?
show the reaction diagram
-
-
-
?
RNA + H2O
RNA(n-1) + nucleoside 5'-phosphate
show the reaction diagram
thymidine 5'-monophosphate p-nitrophenylester + H2O
? + phosphate
show the reaction diagram
-
-
-
?
thymidine 5'-monophosphate p-nitrophenylester + H2O
p-nitrophenol + TMP
show the reaction diagram
-
-
-
?
thymidine 5'-triphosphate + H2O
?
show the reaction diagram
-
-
-
-
?
TpT + H2O + H2O
5'-TMP + thymidine
show the reaction diagram
-
calf
-
?
UDP-glucose + H2O
glucose-1-phosphate + 5'-UMP
show the reaction diagram
uridylyl-3',5'-adenosine + H2O
?
show the reaction diagram
-
FS-44: bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
-
-
?
UTP + H2O
UMP + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O
AMP + diphosphate
show the reaction diagram
-
-
-
-
?
diadenosine polyphosphate + H2O
AMP
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(NH4)2SO4
-
slight activation
Ca2+/calmodulin
-
increased activity ca. 2.3fold
Cu2+
-
activation
Divalent cations
Fe2+
-
contains 0.17 Fe2+ per protein molecule
MgCl2
-
activation
Monovalent cations
-
activation with high concentrations
NH4Cl
-
slight activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(5Z)-5-(2,3,4-trihydroxybenzylidene)-1,3-thiazolidine-2,4-dithione
-
-
(6-methyl-5,11-dioxo-6,11-dihydro-5H-indeno[1,2-c]-isoquinolin-3-yl)carbamic acid
-
-
(RP)-adenosine 5'-(alpha-thio-beta,gamma-dichloromethylene)triphosphate
-
-
(RP)-adenosine 5'-(alpha-thio-beta,gamma-methylene)triphosphate
-
-
(SP)-adenosine 5'-(alpha-thio-beta,gamma-dichloromethylene)triphosphate
-
-
(SP)-adenosine 5'-(alpha-thio-beta,gamma-methylene)triphosphate
-
-
1,2,3,4-tetrahydro-9H-[1]benzothieno[2',3':4,5]pyrimido[6,1-b]quinazolin-9-one
1,2-dimethyl-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
1,3-bis(4-nitrophenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
1-(4-chlorophenyl)-3-(4-methylphenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
1-(4-chlorophenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
1-(4-methoxyphenyl)-3-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
1-(4-methoxyphenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
1-(4-methylphenyl)-3-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
1-(4-methylphenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
1-(furan-2-ylmethyl)-3-(4-methylphenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10,12-dibromo-1,3-bis(4-nitrophenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10,12-dibromo-1-(4-chlorophenyl)-3-(4-methylphenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10,12-dibromo-1-(4-methoxyphenyl)-3-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10,12-dibromo-1-(4-methylphenyl)-3-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10,12-dibromo-1-(furan-2-ylmethyl)-3-(4-methylphenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10,12-dibromo-3-(4-chlorophenyl)-1-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10,12-dibromo-3-(4-methoxyphenyl)-1-(3-nitrophenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10-bromo-1,3-bis(4-nitrophenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10-bromo-1-(4-chlorophenyl)-3-(4-methylphenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10-bromo-1-(4-methoxyphenyl)-3-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10-bromo-1-(4-methylphenyl)-3-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10-bromo-1-(furan-2-ylmethyl)-3-(4-methylphenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10-bromo-3-(4-chlorophenyl)-1-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
10-bromo-3-(4-methoxyphenyl)-1-(3-nitrophenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
11,13-dibromo-1,2,3,4-tetrahydro-9H-[1]benzothieno[2',3':4,5]pyrimido[6,1-b]quinazolin-9-one
11-bromo-1,2,3,4-tetrahydro-9H-[1]benzothieno[2',3':4,5]pyrimido[6,1-b]quinazolin-9-one
2-((6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5H-indeno-[1,2-c]isoquinolin-3-yl)amino)acetic acid
-
-
2-(3-(dimethylamino)propoxy)-3-methoxy-6-(3-morpholinopropyl)-5H-[1,3]dioxolo[4',5':5,6]indeno[1,2-c]isoquinoline-5,12(6H)-dione
-
-
2-(3-(ethylamino)propoxy)-3-methoxy-6-(3-morpholinopropyl)-5H-[1,3]dioxolo[4',5':5,6]indeno[1,2-c]isoquinoline-5,12(6H)-dione
-
-
2-(3-aminopropoxy)-3-methoxy-6-(3-morpholinopropyl)-5H-[1,3]dioxolo[4',5':5,6]indeno[1,2-c]isoquinoline-5,12(6H)-dione
-
-
2-crotonyloxymethyl-(4R,5R,6R)-trihydroxy-cyclohex-2-enone
-
tumor cell line
2-mercaptoethanol
3,20-dioxopregn-4-en-21-yl 4-bromobenzenesulfonate
-
-
3-(4-chlorophenyl)-1-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
3-(4-methoxy-phenyl)-1-(3-nitro-phenyl)-8Hpyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
-
-
3-(4-methoxyphenyl)-1-(3-nitrophenyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
3-amino-6-(3-aminopropyl)-5,6-dihydro-9-methoxy-5,11-dioxo-11H-indeno[1,2-c]isoquinoline dihydrochloride
-
-
3-amino-6-(3-aminopropyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
3-cyano-6,11-dihydro-5,11-dioxo-6-[3-(dimethylaminopropyl)-5H-indeno[1,2-c]isoquinoline]
-
-
-
3-iodo-9-methoxy-6-(3-(dimethylamino)propyl)-5H-indeno-[1,2-c]isoquinoline-5,11(6H)-dione
-
-
3-isobutyl-1-methylxanthine
-
i.e. IBMX
3-methoxy-6-(3-morpholinopropyl)-2-(3-(piperidin-1-yl)-propoxy)-5H-[1,3]dioxolo[4',5':5,6]indeno[1,2-c]isoquinoline-5,12(6H)-dione
-
-
3-methoxy-6-(3-morpholinopropyl)-2-(3-(pyrrolidin-1-yl)-propoxy)-5H-[1,3]dioxolo[4',5':5,6]indeno[1,2-c]isoquinoline-5,12(6H)-dione
-
-
4-[(6-(3-((tert-butoxycarbonyl)amino)propyl)-5,11-dioxo-6,11-dihydro-5H-indeno[1,2-c]isoquinolin-3-yl)amino]-4-oxobutanoic acid
-
-
5'-deoxyribonucleotides
5'-nucleotides
5'-ribonucleotides
5-[3-benzyloxyphenyl]-1,3,4-oxadiazole-2(3H)-thione
5-[3-hydroxyphenyl]-1,3,4-oxadiazole-2(3H)-thione
-
-
5-[4-(t-butyldimethylsilyloxy)-phenyl]-1,3,4-oxadiazole-2(3H)-thione
5-[4-(t-butyldimethylsilyloxy)-phenyl]-1,3,4-thiadiazole-2(3H)-thione
-
-
5-[4-benzyloxyphenyl]-1,3,4-oxadiazole-2(3H)-thione
5-[4-benzyloxyphenyl]-3-octyl-1,3,4-oxadiazole-2(3H)-thione
-
-
5-[4-hydroxyphenyl]-1,3,4-oxadiazole-2(3H)-thione
-
-
5-[4-hydroxyphenyl]-1,3,4-thiadiazole-2(3H)-thione
5-[4-pyridyl]-1,3,4-oxadiazole-2(3H)-thione
-
-
5-[4-pyridyl]-1,3,4-thiadiazole-2(3H)-thione
6,6'-[propane-1,3-diylbis(iminopropane-3,1-diyl)]bis(5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione)
-
-
6-(10-aminodecyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(11-aminoundecyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(12-aminododecyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(2-aminoethyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(3-(1H-imidazol-1-yl)propyl)-8-methoxy-3-nitro-5Hindeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(3-aminopropyl)-3-(methylamino)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(3-aminopropyl)-3-nitro-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
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-
6-(3-aminopropyl)-5,6-dihydro-8-methoxy-3-nitro-5,11-dioxo-11H-indeno[1,2-c]isoquinoline
-
-
6-(3-aminopropyl)-5,6-dihydro-9-methoxy-3-iodo-5,11-dioxo-11H-indeno[1,2-c]isoquinoline
-
-
6-(3-aminopropyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(3-aminopropyl)-7-methoxy-3-nitro-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(3-aminopropyl)-9-methoxy-3-nitro-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(4-aminobutyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(5-aminopentyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
-
-
6-(6-aminohexyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
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-
6-(7-aminoheptyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
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-
6-(8-aminooctyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
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-
6-(9-aminononyl)-5H-indeno[1,2-c]isoquinoline-5,11(6H)-dione
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-
6-[(6-(3-((tert-butoxycarbonyl)amino)propyl)-5,11-dioxo-6,11-dihydro-5H-indeno[1,2-c]isoquinolin-3-yl)amino]-6-oxohexanoic acid
-
-
8-methoxymethyl isobutylmethylxanthine
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20 microM, 8-MM-IBMX, a selective PDE1 inhibitor, no effect on either JG cell cAMP content or renin release compared to untreated controls
8-thioadenosine 5'-triphosphate
-
-
9,11-dibromo-1,2-dimethyl-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
9,11-dibromo-1-(2-furyl)-3-(4-tolyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
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-
9,11-dibromo-1-(4-chlorophenyl)-3-(4-tolyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
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-
9,11-dibromo-1-(4-chlorophenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
9,11-dibromo-1-(4-methoxyphenyl)-3-phenyl-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
-
-
9,11-dibromo-1-(4-methoxyphenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
9,11-dibromo-1-(4-methylphenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
9-bromo-1,2-dimethyl-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
9-bromo-1-(4-chlorophenyl)-3-(4-tolyl)-8H-pyrido[2',3':4,5]pyrimido[6,1-b]quinazolin-8-one
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-
9-bromo-1-(4-chlorophenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
9-bromo-1-(4-methoxyphenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
9-bromo-1-(4-methylphenyl)-7H-thieno[2',3':4,5]pyrimido[6,1-b]quinazolin-7-one
adenosine 5'-(gamma-thio-beta,gamma-methylene)triphosphate
-
-
ADP-ribose
-
competitive to diuridine tetraphosphate
ADPgammaS
-
-
alpha,beta-methylene-adenosine triphosphate
-
inhibition in decreasing order: alpha,beta-methyl-adenosine triphosphate, ATP, UTP, GTP, CTP
alpha,beta-methylene-ADP
-
-
ascorbic acid
-
slight inhibition
ATPgammaS
-
-
beta,gamma-methylene-ATP
-
-
bis(1,3,4-oxadiazol-2-methyl)-5-thione
-
-
bis(1,3,4-oxadiazol-2-propyl)-5-thione
bis(1,3,4-thiadiazole-2-methyl)-5-thione
-
-
cAMP
-
3 mM, 86% residual activity
Citric acid
-
slight inhibition
CTP
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inhibition in decreasing order: alpha,beta-methylene-adenosine triphosphate, ATP, UTP, GTP, CTP
cystine
-
slight inhibition
diadenosine polyphosphate
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competitive
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diethyl dicarbonate
-
-
diisopropyl fluorophosphate
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bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
diphosphate
-
slight inhibition
dipyramidole
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0.1 mM, inhibition, no inhibition at 0.003 mM
dithiothreitol
dofetilide
-
-
ethyl 2-[(6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5Hindeno[1,2-c]isoquinolin-3-yl)amino]acetate
-
-
-
Fe2+
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bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
Fe3+
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bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase, 50% inhibition
fluoride
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-
furamidine
-
-
-
glutathione
glycine
guanosine 5'-tetraphosphate
-
competitive
heparin
-
competitive
IC86340
-
a PDE1 inhibitor, significantly reduces collagen I in human saphenous vein explants undergoing spontaneous remodeling via ex vivo culture, and acts synergistically with bicarbonate, in the absence of bicarbonate of IC86340, itself has no effect on collagen I protein. PDE1C but not PDE1A is the major isoform responsible for mediating the effects of IC86340. Lysosome inhibitors,. i.e. 2',4'-dichlorobenzamil, a nonselective CNG channel blocker, and the more specific CNG channel blocker, L-cisdiltiazem, block IC86340-mediated collagen I reduction, overview
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indomethacin
isobutylmethylxanthine
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100 microM, a nonselective PDE1 inhibitor, increases cAMP and renin relase by 72% and 118%, respectively
KF
-
slight inhibition
KH2PO4
-
slight inhibition
L-cysteine
methyl 2-[(6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5Hindeno[1,2-c]isoquinolin-3-yl)amino]-2-oxoacetate
-
-
methyl 3-[(6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5Hindeno[1,2-c]isoquinolin-3-yl)amino]-3-oxopropanoate
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-
methyl 4-[(6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5Hindeno[1,2-c]isoquinolin-3-yl)amino]-4-oxobutanoate
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-
methyl 5-[(6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5Hindeno[1,2-c]isoquinolin-3-yl)amino]-5-oxopentanoate
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-
methyl 6-[(6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5Hindeno[1,2-c]isoquinolin-3-yl)amino]-6-oxohexanoate
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-
methyl-3,4-dephostatin
a dephostatin derivative, structure-activity relationship analogues of this active compound that have a chemical substitution at a single position show a dramatic decrease in inhibition activity
N-(2-[1-[2-(2-methyl-1H-imidazol-1-yl)pyrimidin-4-yl]piperidin-4-yl]ethyl)sulfuric diamide
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-
N-(2-[1-[6-(2-methyl-1H-imidazol-1-yl)pyrimidin-4-yl]piperidin-4-yl]ethyl)sulfuric diamide
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-
N-(6-Aminohexyl)-5-chloro-1-naphthalenesulfonamide
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W7, 10 microM, a calcium-calmodulin inhibitor
N-ethylmaleimide
N-[2-(1-[6,7-dimethoxy-2-[(E)-2-(pyridin-3-yl)ethenyl]quinazolin-4-yl]piperidin-4-yl)ethyl]sulfuric diamide
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-
N-[2-[1-(2-ethyl-6,7-dimethoxyquinazolin-4-yl)piperidin-4-yl]ethyl]sulfuric diamide
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-
N-[2-[1-(6,7-dimethoxyquinazolin-4-yl)piperidin-4-yl]ethyl]sulfuric diamide
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enzyme binding, structure modelling, overview
N-[2-[1-(6-chloroquinazolin-4-yl)piperidin-4-yl]ethyl]sulfuric diamide
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-
N-[2-[1-(7-chloroquinazolin-4-yl)piperidin-4-yl]ethyl]sulfuric diamide
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-
N-[2-[1-(7-methoxyquinazolin-4-yl)piperidin-4-yl]ethyl]sulfuric diamide
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-
N-[6-(3-aminopropyl)-5,11-dioxo-6,11-dihydro-5H-indeno[1,2-c]isoquinolin-3-yl]acetamide
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-
Na3AsO4
-
slight inhibition
NADH
-
-
NADPH
-
-
NaF
-
slight inhibition
Naphthomycin
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tumor cell line
neomycin B
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a aminoglycoside
NSC 88915
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a steroid inhibitor
NSC88915
nucleoside 5'-triphosphate
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-
nucleoside 5'monophosphate
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-
nucleoside-5'-diphosphate
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-
O-methyl O-(4-nitrophenyl) hydrogen phosphorothioate
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-
o-phenanthroline
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99% inhibition
p-chloromercuribenzoate
-
slight inhibition
p-Diazobenzenesulfonic acid
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DASA: 84% inhibition
phenylalanine
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5 mM, 87% residual activity
phenylmethylsulfonyl fluoride
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FS-44: 5'-PDase activity of bifunctional enzyme: cyclic-ribonucleotide phosphomutase-5'-phosphodiesterase
phosphate
-
slight inhibition
Reducing agents
RNA
-
competitive
rolipram
-
a PDE 4 inhibitor
sodium orthovanadate
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-
suramin
terminal 3'-monophosphoryl group of substrates
trequinsin
-
a PDE 3 inhibitor
tungstate
-
-
Urea
-
slight inhibition
vinpocetin
-
10 mU, surpresses the increase of activity, caused by the addition of 0.2 mM Ca2+
vinpocetine
ZnSO4
-
slight inhibition
additional information