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Information on EC 3.1.3.91 - 7-methylguanosine nucleotidase and Organism(s) Homo sapiens and UniProt Accession Q9H0P0

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.91 7-methylguanosine nucleotidase
IUBMB Comments
The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
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This record set is specific for:
Homo sapiens
UNIPROT: Q9H0P0
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
cniii-like, cg3362, cytosolic nucleotidase iii-like, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cytosolic 5'-nucleotidase 3
-
cNIII-like
-
-
-
-
cytosolic nucleotidase III-like
-
-
-
-
N7-methylguanylate 5'-phosphatase
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
N7-methyl-GMP phosphohydrolase
The enzyme also has low activity with N7-methyl-GDP, producing N7-methyl-GMP. Does not accept AMP or GMP, and has low activity with UMP.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N7-methyl-GDP + H2O
N7-methyl-guanosine + 2 phosphate
show the reaction diagram
-
37fold less efficient than reaction with N7-methyl-GMP
-
?
N7-methyl-GMP + H2O
N7-methyl-guanosine + phosphate
show the reaction diagram
-
enzyme cleaves m7GMP to 7-methylguanosine and phosphate
-
?
additional information
?
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphate
stimulation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0078
N7-methyl-GMP
pH 7.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.24
N7-methyl-GMP
pH 7.5, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
N7-methyl-GMP
pH 7.5, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
5NT3A_HUMAN
336
1
37948
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
analytical ultracentrifugation
34400
34400, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
34400, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Buschmann, J.; Moritz, B.; Jeske, M.; Lilie, H.; Schierhorn, A.; Wahle, E.
Identification of Drosophila and human 7-methyl GMP-specific nucleotidases
J. Biol. Chem.
288
2441-2451
2013
Drosophila melanogaster (Q9W197), Homo sapiens (Q9H0P0), Homo sapiens
Manually annotated by BRENDA team