Information on EC 3.1.3.9 - glucose-6-phosphatase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
3.1.3.9
-
RECOMMENDED NAME
GeneOntology No.
glucose-6-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
D-glucose 6-phosphate + H2O = D-glucose + phosphate
show the reaction diagram
kinetic mechanism applicable generally to both hydrolytic and synthetic activities is a specific adaption of the generally applicable kinetic mechanism for enzymes with modified ping pong bi bi kinetics
-
D-glucose 6-phosphate + H2O = D-glucose + phosphate
show the reaction diagram
reaction mechanism: substrate-transport model
-
D-glucose 6-phosphate + H2O = D-glucose + phosphate
show the reaction diagram
two proposed concepts of structure-function relationships for enzyme, According to the substrate transport-catalytic unit concept, enzyme is a multicomponent system consisting of a fairly non-specific catalytic unit with its active site located on the lumental side of endoplasmic reticulum, and at least three transmembrane-spanning translocases that confer specificity to this system by allowing selective substrates/products access to, or egress from, the sequestered catalytic unit. According to the combined conformational flexibility-substrate transport concept, enzyme is a multifunctional enzyme embedded within the endoplasic reticulum membrane that possess both catalytic and substrate/product transport activities.
-
D-glucose 6-phosphate + H2O = D-glucose + phosphate
show the reaction diagram
reaction mechanism, His176 is the amino acid in enzyme that acts as the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis
-
D-glucose 6-phosphate + H2O = D-glucose + phosphate
show the reaction diagram
The enzyme family comprises two active isozyme: the D-glucose 6-phosphatase-alpha, in which is His176 the amino acid in enzyme that acts as the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis and the D-glucose 6-phosphatase-alpha, in which is His167 the amino acid in enzyme that acts as the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis. Both enzyme-alpha and enzyme-beta couple with the D-glucose 6-phosphate transporter to form an active enzyme complex
-
D-glucose 6-phosphate + H2O = D-glucose + phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Galactose metabolism
-
gluconeogenesis III
-
Glycolysis / Gluconeogenesis
-
Metabolic pathways
-
Starch and sucrose metabolism
-
SYSTEMATIC NAME
IUBMB Comments
D-glucose-6-phosphate phosphohydrolase
Wide distribution in animal tissues. Also catalyses potent transphosphorylations from carbamoyl phosphate, hexose phosphates, diphosphate, phosphoenolpyruvate and nucleoside di- and triphosphates, to D-glucose, D-mannose, 3-methyl-D-glucose or 2-deoxy-D-glucose [cf. EC 2.7.1.62 (phosphoramidate---hexose phosphotransferase), EC 2.7.1.79 (diphosphate---glycerol phosphotransferase) and EC 3.9.1.1 (phosphoamidase)].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-glucose-6-phosphate phosphorylase
-
-
G-6-Pase
-
-
-
-
G-6-Pase
-
-
G6Pase
-
-
-
-
G6Pase
-
-
G6Pase
Q9Z186
-
G6Pase
-
-
G6Pase
Q8AYI6
-
G6Pase
Q98UF8
-
G6Pase-alpha
P35575
-
G6PC
-
-
G6PC
P35575
-
G6PC
-
-
G6PC
P35576
-
G6PC-2
-
-
G6PC-3
-
-
G6Pc1
Q19KA1
-
G6PC2
-
-
G6PC2
Q9NQR9
-
G6PC2
Q9Z186
-
Glc-6-P phosphohydrolase
-
-
Glc-6-Pase-alpha
-
-
Glc-6-Pase-beta
-
-
glucose 6-phosphatase
-
-
glucose 6-phosphate phosphatase
-
-
-
-
glucose 6-phosphate phosphohydrolase
-
-
-
-
glucose-6-phosphatase
-
-
glucose-6-phosphatase
-
-
glucose-6-phosphatase
Q19KA1
-
glucose-6-phosphatase
-
-
glucose-6-phosphatase
-
-
glucose-6-phosphatase
-
-
glucose-6-phosphatase
-, Q98UF8
-
glucose-6-phosphatase catalytic subunit 2
Q9NQR9
-
glucose-6-phosphatase catalytic subunit-related protein
-
-
glucose-6-phosphatase catalytic subunit-related protein
-
-
glucose-6-phosphatase catalytic unit 2
-
-
glucose-6-phosphatase-alpha
-
-
glucose-6-phosphatase-alpha
P35575
-
IGRP
Q9NQR9
-
IGRP
-
-
IGRP
Q9Z186
-
islet-specific glucose-6-phosphatase catalytic subunit-related protein
-
-
islet-specific glucose-6-phosphatase catalytic subunit-related protein
-
-
islet-specific glucose-6-phosphatase catalytic subunit-related protein
Q9Z186
-
islet-specific glucose-6-phosphatase-related protein
Q9NQR9
-
phosphatase, glucose 6-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9001-39-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
female and male mongrel dogs
-
-
Manually annotated by BRENDA team
i.e. pigeon
-
-
Manually annotated by BRENDA team
European sea bass
-
-
Manually annotated by BRENDA team
; wild-type strain AX4 and several mutant strains
-
-
Manually annotated by BRENDA team
Frog
-
-
-
Manually annotated by BRENDA team
; individuals from Argentina
UniProt
Manually annotated by BRENDA team
; type 1 diabetes individuals and individuals with a DR0301 or DR0401 haplotype, which are disease susceptible
-
-
Manually annotated by BRENDA team
; female woodchuck
-
-
Manually annotated by BRENDA team
woodchuck model of hepatitis virus-induced hepatocellular carcinoma
-
-
Manually annotated by BRENDA team
Marmota sp.
-
-
-
Manually annotated by BRENDA team
two enzyme forms with different acid sensitivity and tissue location
-
-
Manually annotated by BRENDA team
Pachnoda ephippiata
-
-
-
Manually annotated by BRENDA team
Phryganea sp.
-
-
-
Manually annotated by BRENDA team
male Wistar rats
-
-
Manually annotated by BRENDA team
the rats are divided into the follwing age groups: 0.5, 1,2.4.8.12.20 and 28 month of age
-
-
Manually annotated by BRENDA team
salamander
-
-
-
Manually annotated by BRENDA team
Sarcophaga barbata
-
-
-
Manually annotated by BRENDA team
gilthead sea bream
SwissProt
Manually annotated by BRENDA team
gilthead sea bream
UniProt
Manually annotated by BRENDA team
Turtle
-
-
-
Manually annotated by BRENDA team
Vespa vulgaris
-
-
-
Manually annotated by BRENDA team
i.e. toad
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
G6PC3 mutation induces major alterations in neutrophil N- and O-glycomes
malfunction
-
expression of hepatic glucose-6-phosphatase-alpha is down-regulated in patients with hepatic steatosis
metabolism
-
an enhanced glucose 6-phosphatase activity in liver of rats exposed to Mg2+-deficient diet is detected
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-2-fluoro-D-glucose 6-phosphate + H2O
2-deoxy-2-fluoro-D-glucose + phosphate
show the reaction diagram
-
18F-labeled model substrate
-
-
ir
2-deoxy-2-fluoro-D-glucose-6-phosphate + H2O
2-deoxy-2-fluoro-D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
2-deoxy-D-glucose + diphosphate
?
show the reaction diagram
-
-
-
-
?
2-Deoxy-D-glucose 6-phosphate + H2O
2-Deoxy-D-glucose + phosphate
show the reaction diagram
Q9BUM1
a better substrate in disrupted vesicles at pH 5.5 and pH 6.5
-
?
2-Deoxy-D-glucose 6-phosphate + H2O
2-Deoxy-D-glucose + phosphate
show the reaction diagram
-
only in disrupted vesicles
-
?
5'-AMP + H2O
?
show the reaction diagram
-
2.6% of the activity with D-glucose 6-phosphate
-
-
?
beta-glycerophosphate + H2O
glycerol + phosphate
show the reaction diagram
-
1.2% of the activity with D-glucose 6-phosphate
-
-
?
carbamoyl-phosphate + glucose
glucose 6-phosphate + NH3 + CO2
show the reaction diagram
-
-
-
?
carbamoyl-phosphate + glucose
glucose 6-phosphate + NH3 + CO2
show the reaction diagram
-
-
-
?
carbamoyl-phosphate + glucose
glucose 6-phosphate + NH3 + CO2
show the reaction diagram
-
-
-
-
?
carbamoyl-phosphate + H2O
phosphate + NH3 + CO2
show the reaction diagram
-
-
-
?
D-galactose + diphosphate
?
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Q9BUM1
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
P35575
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Frog
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Frog
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Turtle
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Turtle
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Marmota sp.
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Q98UF8, -
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
ir
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Q8AYI6
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
best substrate in disrupted and not disrupted vesicles at pH 5.5 and pH 6.5
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
enzyme catalyses the final step in both glycogenolysis and glyconeogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
enzyme is one of the rate-limiting enzymes of hepatic glucogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
enzyme system is required in hepatic D-glucose phosphorylation
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
key enzyme in D-glucose homeostasis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme catalyses the terminal reaction of gluconegenesis and glycogenolysis and plays a major role in the contol of blood glucose levels
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme is required for the final steps of glycogenolysis and gluconeogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme is required for the final steps of glycogenolysis and gluconeogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme plays a important role of providing D-glucose during starvation
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Q98UF8, -
dietary regulation of the enzyme in the liver glucose metabolism, overview
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
key enzyme in glucose metabolism
-
-
ir
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the brain functional glucose-6-phosphatase complex is capable of endogenous glucose production from glucose 6-phosphate, independent of blood glucose
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme catalytic subunit expression is regulated independently from the glucose-6-phosphate transporter expression by metabolites, overview
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme is the key enzyme of the gluconeogenesis pathway, and is positively regulated by a extracellular multipolypeptide complex called counting factor, CF, or the CF component coutin, respectively, while the glucokinase activity is inhibited by CF, regulation overview
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
P35575
G6Pase facilitates microsomal D-glucose 6-phosphate uptake by D-glucose 6-phosphate transporter
-
-
?
D-mannose + diphosphate
?
show the reaction diagram
-
-
-
-
?
D-mannose 6-phosphate + H2O
D-mannose + phosphate
show the reaction diagram
-
-
-
?
diphosphate + glucose
glucose 6-phosphate + phosphate
show the reaction diagram
-
-
-
?
diphosphate + glucose
glucose 6-phosphate + phosphate
show the reaction diagram
-
-
-
?
diphosphate + glucose
glucose 6-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
diphosphate + glucose
glucose 6-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
diphosphate + glucose
glucose 6-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
-
-
?
diphosphate + H2O
2 phosphate
show the reaction diagram
-
23% of the activity with D-glucose 6-phosphate
-
-
?
glucose + ADP
glucose 6-phosphate + AMP
show the reaction diagram
-
-
-
-
?
glucose + ATP
glucose 6-phosphate + ADP
show the reaction diagram
-
-
-
-
?
glucose + CDP
glucose 6-phosphate + CMP
show the reaction diagram
-
-
-
-
?
glucose + CTP
glucose 6-phosphate + CDP
show the reaction diagram
-
-
-
-
?
glucose + dCTP
glucose 6-phosphate + dCDP
show the reaction diagram
-
-
-
-
?
glucose + GDP
glucose 6-phosphate + GMP
show the reaction diagram
-
-
-
-
?
glucose + GTP
glucose 6-phosphate + GDP
show the reaction diagram
-
-
-
-
?
glucose + ITP
glucose 6-phosphate + IDP
show the reaction diagram
-
-
-
-
-
glucose + ITP
glucose 6-phosphate + IDP
show the reaction diagram
-
-
-
-
?
glucose + phosphoenolpyruvate
glucose 6-phosphate + pyruvate
show the reaction diagram
-
-
-
-
?
glucose + phosphoramide
glucose + aminophosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
Q9BUM1
best substrate in disrupted and not disrupted vesicles at pH 5.5 and pH 6.5
-
?
p-nitrophenylphosphate + H2O
p-nitrophenol + phosphate
show the reaction diagram
-
1.4% of the activity with D-glucose 6-phosphate
-
-
?
glycerol + diphosphate
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
activities for EC 3.1.3.9, EC 3.6.1.1, and pyrophosphate-glucose phosphotransferase are due to a single enzyme
-
-
-
additional information
?
-
-
the biosynthetic activity of the enzyme may act in concert with glycogen synthase during amino-acid-induced glycogenesis from glucose. Amino acids may play a role in increasing biosynthetic activity of the enzyme
-
-
-
additional information
?
-
-
the enzyme probably plays a key role in the down-regulation of the calcium signal required for glycogen mobilization
-
-
-
additional information
?
-
-
the role of the enzyme in muscle is either to produce glucose from glucose 6-phosphate derived from glycogen or to provide the enzymatic basis for a substrate cycle betwen glucose and glucose 6-phosphate in muscle to improve the sensitivity of the mechanism that regulates the rate of glucose phosphorylation
-
-
-
additional information
?
-
-
the enzyme is involved in sleep
-
-
-
additional information
?
-
-
the main regulatory function of the enzyme is to buffer the glucose 6-phosphate concentration. Hyperglycemia stimulates glucose-6-phosphatase gene transcription
-
-
-
additional information
?
-
-
the activity of glucose-6-phosphate synthesis can serve as an adjunct to glucokinase in hepatic glucose phosphorylation and may function in liver-mediated, adjustable maintanance of blood glucose homeostasis
-
-
-
additional information
?
-
-
the enzyme is involved in the mobilization of glucose from liver cells
-
-
-
additional information
?
-
-
final step in gluconeogenesis
-
-
-
additional information
?
-
-
terminal reaction of the pathway of glycogenolysis and gluconeogenesis. Activity of the enzyme is influenced by changes of the phospholipids on the microsomal membrane
-
-
-
additional information
?
-
-
the multicomponent hepatic glucose 6-phosphatase system catalyzes the terminal step of the hepatic glucose production and plays a role in the regulation of blood glucose
-
-
-
additional information
?
-
-
cells of Dictyostelium discoideum form groups of about 20000 cells. The group size is regulated in part by a negative feedback pathway mediated by a secreted multipolypeptide complex called counting factor. It appears that part of the counting factor signal transduction pathway involves inhibiting the activity of glucose-6-phosphatase, decreasing intracellular glucose levels and affecting the levels of other metabolites, to regulate group size
-
-
-
additional information
?
-
-
endoplasmic stress may play a role in diabetes, and it results in increased enzyme activity in the liver, overview
-
-
-
additional information
?
-
P35575
enzyme deficiency causes glycogen storage disease type Ia, GSD-Ia, overview, glucose-6-phosphatase mutations affect protein stability and cause glycogen storgae disease type Ia, GSD-Ia, determined in Argentina, overview
-
-
-
additional information
?
-
-
reduced enzyme activity in woodchuck model of hepatitis virus-induced hepatocellular carcinoma, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
P35575
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
enzyme catalyses the final step in both glycogenolysis and glyconeogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
enzyme is one of the rate-limiting enzymes of hepatic glucogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
enzyme system is required in hepatic D-glucose phosphorylation
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
key enzyme in D-glucose homeostasis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme catalyses the terminal reaction of gluconegenesis and glycogenolysis and plays a major role in the contol of blood glucose levels
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme is required for the final steps of glycogenolysis and gluconeogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme is required for the final steps of glycogenolysis and gluconeogenesis
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme plays a important role of providing D-glucose during starvation
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
Q98UF8, -
dietary regulation of the enzyme in the liver glucose metabolism, overview
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
key enzyme in glucose metabolism
-
-
ir
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the brain functional glucose-6-phosphatase complex is capable of endogenous glucose production from glucose 6-phosphate, independent of blood glucose
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme catalytic subunit expression is regulated independently from the glucose-6-phosphate transporter expression by metabolites, overview
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
the enzyme is the key enzyme of the gluconeogenesis pathway, and is positively regulated by a extracellular multipolypeptide complex called counting factor, CF, or the CF component coutin, respectively, while the glucokinase activity is inhibited by CF, regulation overview
-
-
?
additional information
?
-
-
the biosynthetic activity of the enzyme may act in concert with glycogen synthase during amino-acid-induced glycogenesis from glucose. Amino acids may play a role in increasing biosynthetic activity of the enzyme
-
-
-
additional information
?
-
-
the enzyme probably plays a key role in the down-regulation of the calcium signal required for glycogen mobilization
-
-
-
additional information
?
-
-
the role of the enzyme in muscle is either to produce glucose from glucose 6-phosphate derived from glycogen or to provide the enzymatic basis for a substrate cycle betwen glucose and glucose 6-phosphate in muscle to improve the sensitivity of the mechanism that regulates the rate of glucose phosphorylation
-
-
-
additional information
?
-
-
the enzyme is involved in sleep
-
-
-
additional information
?
-
-
the main regulatory function of the enzyme is to buffer the glucose 6-phosphate concentration. Hyperglycemia stimulates glucose-6-phosphatase gene transcription
-
-
-
additional information
?
-
-
the activity of glucose-6-phosphate synthesis can serve as an adjunct to glucokinase in hepatic glucose phosphorylation and may function in liver-mediated, adjustable maintanance of blood glucose homeostasis
-
-
-
additional information
?
-
-
the enzyme is involved in the mobilization of glucose from liver cells
-
-
-
additional information
?
-
-
final step in gluconeogenesis
-
-
-
additional information
?
-
-
terminal reaction of the pathway of glycogenolysis and gluconeogenesis. Activity of the enzyme is influenced by changes of the phospholipids on the microsomal membrane
-
-
-
additional information
?
-
-
the multicomponent hepatic glucose 6-phosphatase system catalyzes the terminal step of the hepatic glucose production and plays a role in the regulation of blood glucose
-
-
-
additional information
?
-
-
cells of Dictyostelium discoideum form groups of about 20000 cells. The group size is regulated in part by a negative feedback pathway mediated by a secreted multipolypeptide complex called counting factor. It appears that part of the counting factor signal transduction pathway involves inhibiting the activity of glucose-6-phosphatase, decreasing intracellular glucose levels and affecting the levels of other metabolites, to regulate group size
-
-
-
additional information
?
-
-
endoplasmic stress may play a role in diabetes, and it results in increased enzyme activity in the liver, overview
-
-
-
additional information
?
-
P35575
enzyme deficiency causes glycogen storage disease type Ia, GSD-Ia, overview, glucose-6-phosphatase mutations affect protein stability and cause glycogen storgae disease type Ia, GSD-Ia, determined in Argentina, overview
-
-
-
additional information
?
-
-
reduced enzyme activity in woodchuck model of hepatitis virus-induced hepatocellular carcinoma, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Cd2+
-
G6Pase is not a target of Cd2+ insult, the G6Pase activity measured at 37C is high only in 1-month Cd2+-treated group (0.84mg/kg, 35% increase of activity), in 1-week Cd2+-treated group measurements of G6Pase activity at 25C show marginal increase
Mg2+
-
reaction carried out in the absence or presence of 1 mM MgCl2
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-penten-3-one
-
-
1-propyl-3-(pyridyn-2-yl)thiourea
-
40.71% inhibition
-
1-[(4'-O-(E)-p-coumaroyl)-beta-D-glucopyranosyl]-oxy-2-phenol
-
-
1-[(6'-O-(E)-p-coumaroyl)-beta-D-glucopyranosyl]-oxy-2-phenol
-
-
1-[O-beta-D-glucopyranosyl(1->2)-beta-D-glucopyranosyl]-oxy-2-phenol
-
-
2-oxoglutarate
-
in complex with Mg2+, inhibition at low substrate concentrations, inhibition of the translocase component of the glucose 6-phosphatase system
2-{[bis(hydrazine)methylene]amino}-4-methylpentanoic acid
-
2.61% inhibition
-
2-{[bis(hydrazino)methylene]amino}-3-(4-hydroxyphenyl)propanoic acid
-
6.61% inhibition
-
2-{[bis(hydrazino)methylene]amino}-3-hydroxybutanoic acid
-
11.68% inhibition
-
2-{[bis(hydrazino)methylene]amino}-3-hydroxypropanoic acid
-
10.56% inhibition
-
2-{[bis(hydrazino)methylene]amino}-3-methylpentanoic acid
-
3.37% inhibition
-
3,4-dicaffeoylquinic acid
-
-
3,4-dichlorobenzaldehyde dibenzylhydrazone
-
-
3-Mercaptopicolinic acid
-
inhibitor of D-glucose 6-phosphate translocase
3-methoxybenzaldehyde dibenzylhydrazone
-
-
4,4'-di-isothiocyanostilbene-2,2'-disulphonate
-
inhibitor of D-glucose 6-phosphate translocase
4,5-dicaffeoylquinic acid
-
-
4-(beta-D-glucopyranosyloxy) benzoic acid
-
-
4-caffeoylquinic acid
-
-
4-chlorobenzaldehyde dibenzylhydrazone
-
-
4-hydroxybenzaldehyde dibenzylhydrazone
-
-
4-maleimidylstilbene-2,2'-disulfonic acid
-
-
4-methoxybenzaldehyde dibenzylhydrazone
-
-
4-methoxyphenyl-[4-(4-methoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
-
4-methoxyphenyl-[4-(4-trifluoromethoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
-
5-((((iodoacetyl)amino)ethyl)amino)naphtalene-1-sulfonic acid
-
-
-
5-caffeoylquinic acid
-
-
5-chlorotetrahydrothiophene-2-carbaldehyde dibenzylhydrazone
-
-
5-ethyltetrahydrofuran-2-carbaldehyde dibenzylhydrazone
-
-
alpha-phosphatidylinositol 3-monophosphate
-
-
alpha-phosphatidylinositol 4,5-diphosphate
-
-
alpha-phosphatidylinositol 4-monophosphate
-
-
apigenin-7-O-beta-D-glucopyranoside
-
-
apigenin-7-O-beta-D-glucuronic acid-6''-methyl ester
-
-
Arachidonoyl-CoA
-
-
behenoyl-CoA
-
-
benzaldehyde dibenzylhydrazone
-
-
chlorogenic acid
-
inhibitor of D-glucose 6-phosphate translocase
chlorogenic acid
-
-
CoA
-
inhibits enzyme activity in intact microsomes
D-glucose
-
non-competitive inhibitor, irrespective of the presence of detergents
D-glucose
-
reduces the G6Pase activity both in intact and permeabilized liver microsomes in a concentration-dependent manner. Extent of inhibition is comparable with that achieved by epigallocatechin gallate in intact microsomes
diphosphate
-
-
EGTA
-
inhibits enzyme activity in histone 2A-treated or alamethicin-treated microsomes, but has little effect on enzyme activity in intact microsomes
Epicatechin gallate
-
acts similarly but less efficiently than epigallocatechin gallate
epigallocatechin gallate
-
increases latency of G6Pase, inhibitory effect is greater in intact than in permeabilized microsomes. It does not hinder microsomal D-glucose 6-phosphate uptake. Reduces activity of G6Pase through the inhibition of glucose efflux from the endoplasmic reticulum lumen; inhibition of enzyme in intact, but not in permeabilized microsome. Epigallocatechin gallate does not hinder microsomal glucose uptake, but inhibits microsomal glucose efflux thus inhibiting the enzyme through an elevated luminal glucose level
fatty-acyl-CoA
-
with chain-length equal to or higher than 16, at 0.001-0.002 mM, the inhibitory effect on the enzyme of untreated microsomes is either partially or totally cancelled, or even changes into an activation effect at higher concentrations, the inhibition is fully reversible in presence of bovine serum albumin. With medium chain-length, 10-14 carbons inhibit the enzyme of untreated microsomes in a dose-dependent manner in the range 0.001-0.02 mM, the higher the chain length, the stronger the inhibitory effect
gallocatechin gallate
-
increases latency of G6Pase, inhibitory effect is greater in intact than in permeabilized microsomes
Insulin
-
insulin causes a decrease in the activity of enzyme in the liver in vivo
-
isonicotinaldehyde dibenzylhydrazone
-
-
kodaistatin A
-
A and C
L-alpha-phosphatidylinositol 3,4,5-trisphosphate
-
-
L-alpha-phosphatidylinositol 3,4-diphosphate
-
-
mumbaistatin
-
-
myristoyl-CoA
-
uncompetitive inhibition of enzyme in untreated microsomes, non-competitive inhibition of enzyme from detergent-treated microsomes
N-(pyridine-2-yl)morpholine-4-carbothioamide
-
35.32% inhibition
-
N-(pyridine-2-yl)piperidine-4-carbothioamide
-
38.88% inhibition
-
nicotinaldehyde dibenzylhydrazone
-
-
nitrothiocyanobenzoic acid
-
-
-
orthovanadate
-
-
oxaloacetate
-
in complex with Mg2+, inhibition at low substrate concentrations, inhibition of the translocase component of the glucose 6-phosphatase system
p-hydroxy mercury-phenylsulfonate
-
-
p-hydroxy mercurybenzoic acid
-
-
palmitoyl-CoA
-
inhibits enzyme activity in intact microsomes
pentenone
-
-
-
Phenobarbital
-
the inhibition in dependence on the age of rats is studied
phosphate
-
-
phosphate
-
non-competitive inhibition in intact microsomes, but competitive in the presence of detergents
phosphatidylinositol
-
-
phosphoinositides
-
in order of decreasing efficiency: phosphatidylinositol, L-alpha-phosphatidylinositol 3,4,5-trisphosphate, L-alpha-phosphatidylinositol 3,4-diphosphate, alpha-phosphatidylinositol 4,5-diphosphate, alpha-phosphatidylinositol 3-monophosphate, alpha-phosphatidylinositol 4-monophosphate, phosphatidylinositol. Mechanism of inhibition with alpha-phosphatidylinositol 3,4-diphosphate, alpha-phosphatidylinositol 4,5-diphosphate or L-alpha-phosphatidylinositol 3,4,5-trisphosphate is competitive
-
pyridine-2-carbaldehyde dibenzylhydrazone
-
-
pyrocatechol-O-beta-D-glucopyranoside
-
-
quercetin-3-O-beta-D-glucopyranoside
-
-
S3483
-
reversible inhibitor of the translocase component of the multicomponent hepatic glucose 6-phosphatase system
S3483
-
only depresses G6Pase activity in intact microsomes, thus increases latency
S5627
-
potent linear competitive inhibitor of the translocase component of the multicomponent hepatic glucose 6-phosphatase system
-
silibinin
-
induces a dose-dependent inhibition of gluconeogenesis associated with a potent decrease in enzyme activity; inhibits gluconeogenesis and D-glucose 6-phosphate hydrolysis from either lactate/pyruvate, glycerol or fructose in a dose-dependent manner. Effect is stronger in intact than in permeabilized microsomes
sodium barbital
-
3 mM, complete inhibition
sodium pentobarbital
-
3 mM, complete inhibition
stearoyl-CoA
-
-
Svetol
-
commercial unroasted and decaffeinated green Coffea canephora extract, competive inhibition
-
tetrahydrofuran-2-carbaldehyde dibenzylhydrazone
-
-
tetrahydrothiophene-2-carbaldehyde dibenzylhydrazone
-
-
tosyl-L-Lys-chloromethyl ketone
-
inactivation of the enzyme in intact microsomes, no effect on enzyme in deoxycholate-modified microsomes
tosyl-L-Phe-chloromethyl ketone
-
inactivation of the enzyme in intact microsomes, no effect on enzyme in deoxycholate-modified microsomes
tosyl-lysyl-chloromethane
-
inhibitor of D-glucose 6-phosphate translocase
tosylphenylalanylchloromethane
-
inhibitor of D-glucose 6-phosphate translocase
trans-2-nonenal
-
-
trans-2-pentenal
-
-
tungstate
-
-
vanadate
-
reduces activity both in intact and permeabilized microsomes and decreases latency
additional information
-
the effect of the short-chain aldehydes on the kinetic parameters increases with chain-length
-
additional information
-
tumor necrosis factor inhibits the transcriptional rate of glucose-6-phosphatase in vivo and in vitro
-
additional information
-
a Pro metabolite inhibits glucose 6-phosphate hydrolysis and thus directs glucose 6-phosphate away from glucose production and towards glycogen
-
additional information
-
The enzyme is inhibited by several amphiphilic compounds, such as fatty acids and acyl-CoAs, but the physioligal significance is questionable, since the liver contains a fatty-acyl CoA binding protein, which may well prevent this effect. Some thiol reagents inhibit enzyme activity much more in intact than in disrupted microsomes.
-
additional information
-
a series of N,N-dibenzyl-N-benzylidnenhydrazine represent a novel class of potent competivitve D-glucose-6-phosphatase catalytic inhibitors using disrupted microsomes
-
additional information
-
expression of dominant-negative versions of both cAMP response element-binding protein and CAAT/enhancer-binding protein blocks the glucose response of the proximal region in a dose-dependent manner
-
additional information
-
propyl gallate has no effect
-
additional information
-
activity of glucose-6-phosphatase in crude microsomes from cells with high, normal, or low counting factor activity has a negative correlation with the amount of counting factor present in these cell lines. Molecules greater than 10 kDa from wild-type cells strongly repress activity of glucose-6-phosphatase in wild-type microsomes
-
additional information
-
insulin and epidermal growth factor inhibit basal mouse G6Pase fusion gene transcription
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
beta-Naphthoflavone
-
the activation in dependence on the age of rats is studied
calyculin A
-
activation of enzyme in supernatant and in intact or disrupted microsomes
D-glucose
-
elevated concentration, D-glucose may play a direct role as a stimulator of enzyme expression
D-glucose
-
induces the expression of G-6-Pase at transcriptional and posttranscriptional levels, strongly increases endogenous G-6-Pase mRNA levels in 832/13 cells and in rat pancreatic islets. Induced levels from islets are still markedly lower than in untreated primary hepatocytes
deoxycholate
-
-
dexamethasone
-
in cultured hepatoma cells
dexamethasone
-
the activation in dependence on the age of rats is studied
glycocorticoids
-
injection of glycocorticoids in vivo increases to a modestextent the activity of enzyme in the livers of control and adrenalectomized rats
-
Histone 2A
-
stimulates enzyme activity by permeabilizing the microsomal membrane
-
Lubrol
-
-
-
microcystin-LR
-
activation of enzyme in supernatant and in intact or disrupted microsomes
okadaic acid
-
activation of enzyme in supernatant and in intact or disrupted microsomes
microcystin-RR
-
activation of enzyme in supernatant and in intact or disrupted microsomes
additional information
-
selective stimulation of expression by glucagon in vivo and cAMP in situ, of recombinant enzyme catalytic subunit expressed in human Hep-G2 cells or native enzyme catalytic subunit in pancreatic-clamped or liver-clamped, euglycemic conscious dogs, overview
-
additional information
-
endoplasmic stress, induced by treatment of hepatocytes with tunicamycin and thapsigargin, leads to induction of enzyme activity in hepatocytes, overview
-
additional information
-
the enzyme is positively regulated by a secreted multipolypeptide complex called counting factor, CF, or the CF component coutin, respectively
-
additional information
-
distinct cis-regulatory promoter elements are involved in the glucose response of the rat G-6-Pase gene. Full-length (-4078/+64) promoter confers a moderate glucose response to a reporter construct in HL1C rat hepatoma cells, which is dependent on coexpression of glucokinase. The same construct provides a robust glucose response in 832/13 INS-1 rat insulinoma cells, which are not glucogenic
-
additional information
Q98UF8, -
G6Pase activitiy and gene expression is not influenced by dietary carbohydrates
-
additional information
-
in crude cytosols glucose-6-phosphatase activity has a positive correlation with counting factor accumulation. Molecules greater than 10 kDa from cells with low counting factor activity significantly increase activity of glucose-6-phosphatase in wild-type microsomes. In smlA- and wild-type cells, detergent treatment with Triton X-100 to allow direct access of D-glucose 6-phosphate to the catalytic subunit does not increase enzymatic activity. In low counting factor crude microsomes, detergent treatment increases glucose-6-phosphatase activity; secreted multipolypeptide complex called counting factor regulates one or more proteins greater than 10000 Da in crude cytosol that affect microsome-associated glucose-6-phosphatase activity
-
additional information
-
higher G6Pase activities are recorded in fish fed glucose diet than in fish fed glucose-free diet
-
additional information
Q8AYI6
higher G6Pase activities are recorded in fish fed glucose diet than in fish fed glucose-free diet
-
additional information
P35576
peroxisome proliferator activated receptor gamma coactivator-1alpha stimulates mouse G6pc-luciferase fusion gene expression through hepatocyte nuclear factor-4alpha
-
additional information
-
O-glycosylation of Forkhead Box Other-1 trancription factor increases its transcriptional activity towards the glucose 6-phosphatase gene
-
additional information
-
Foxo1 synergizes with HNF-4 in activating the G6Pase gene transcription
-
additional information
Q9Z186
Foxa2 and MafA but not Pdx-1 directly regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression. Pdx-1, Foxa2, and MafA binding is required for maximal IGRP promoter activity.
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
11.1
-
ADP
-
partially purified enzyme
4.7
-
ATP
-
microsomal enzyme suspension
0.6
-
Carbamoyl phosphate
-
enzyme from pancreas
0.8
-
Carbamoyl phosphate
-
enzyme from adrenals
1.3
-
Carbamoyl phosphate
-
enzyme from testes
1.3
-
Carbamoyl phosphate
-
-
2.5
-
Carbamoyl phosphate
-
enzyme from brain
2.5
-
Carbamoyl phosphate
-
-
3.8
-
CDP
-
microsomal enzyme suspension
3.1
-
CTP
-
microsomal enzyme suspension
0.1
-
D-glucose 6-phosphate
-
cleared lysate of countin- cells with low activity of counting factor; crude cytosol of cells with low counting factor; crude cytosol of countin- cells with low activity of counting factor; crude microsome of countin- cells with low activity of counting factor
0.18
-
D-glucose 6-phosphate
-
crude cytosol of wild-type
0.3
-
D-glucose 6-phosphate
-
pH 6.5, in disrupted vesicles
0.4
-
D-glucose 6-phosphate
-
crude cytosol of smlA- cells; crude microsome of cells with disruption in smlA gene resulting in oversecretion of counting factor
0.5
-
D-glucose 6-phosphate
-
pH 5.5, in disrupted vesicles
0.65
-
D-glucose 6-phosphate
-
crude cytosol of wild-type cells; crude microsome of wild-type
1
-
D-glucose 6-phosphate
Q9BUM1
pH 5.5, in disrupted vesicles
1.2
-
D-glucose 6-phosphate
-
crude cytosol of cells with disruption in smlA gene resulting in oversecretion of counting factor
1.3
-
D-glucose 6-phosphate
-
pH 6.5, in not disrupted vesicles
2
3
D-glucose 6-phosphate
-
-
2
-
D-glucose 6-phosphate
Q9BUM1
pH 6.5, in disrupted vesicles
2.1
-
D-glucose 6-phosphate
-
pH 7.5, coutin mutant enzyme; pH 7.5, wild-type enzyme
2.1
-
D-glucose 6-phosphate
-
cleared lysate of wild-type
2.4
-
D-glucose 6-phosphate
-
pH 7.5, smlA mutant enzyme
2.4
-
D-glucose 6-phosphate
-
ccleared lystae of cells with disruption in smlA gene resulting in oversecretion of counting factor
2.41
-
D-glucose 6-phosphate
-
-
4.1
-
D-glucose 6-phosphate
Q9BUM1
pH 5.5, in not disrupted vesicles
4.3
-
D-glucose 6-phosphate
Q9BUM1
pH 6.5, in not disrupted vesicles
4.8
-
D-glucose 6-phosphate
-
control, intact microsomes
5.1
-
D-glucose 6-phosphate
-
pH 5.5, in not disrupted vesicles
5.3
-
D-glucose 6-phosphate
-
intact microsomes incubated with silibinin
2.6
-
dCTP
-
microsomal enzyme suspension
1.3
-
diphosphate
-
microsomal enzyme suspension
1.8
-
diphosphate
-
partially purified enzyme
2.7
-
GDP
-
partially purified enzyme
0.51
-
glucose 6-phosphate
-
-
0.93
-
glucose 6-phosphate
-
enzyme from activated microsomes
1.2
-
glucose 6-phosphate
-
detergent-treated preparation
1.8
-
glucose 6-phosphate
-
preparation without detergent
1.9
-
glucose 6-phosphate
-
-
2.7
-
glucose 6-phosphate
-
-
3.6
-
GTP
-
microsomal enzyme suspension
6.9
-
phosphoenolpyruvate
-
microsomal enzyme suspension
2.8
-
phosphoramide
-
microsomal enzyme suspension
6.1
-
ITP
-
microsomal enzyme suspension
additional information
-
additional information
-
-
-
additional information
-
additional information
-
the KM is decreased in arthritic rats in comparison to control rats
-
additional information
-
additional information
-
kinetics and in vivo assay development
-
additional information
-
additional information
-
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
700
-
glucose 6-phosphate
-
enzyme in disrupted microsomes
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00128
-
3,4-dichlorobenzaldehyde dibenzylhydrazone
-
-
0.00045
-
3-methoxybenzaldehyde dibenzylhydrazone
-
-
0.035
-
4,4'-di-isothiocyanostilbene-2,2'-disulphonate
-
-
0.00015
-
4-chlorobenzaldehyde dibenzylhydrazone
-
-
0.00154
-
4-hydroxybenzaldehyde dibenzylhydrazone
-
-
0.5
-
4-maleimidylstilbene-2,2'-disulfonic acid
-
-
0.00014
-
4-methoxybenzaldehyde dibenzylhydrazone
-
-
0.00027
-
4-methoxyphenyl-[4-(4-methoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
disrupted microsomes
0.00061
-
4-methoxyphenyl-[4-(4-methoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
intact microsomes
0.00154
-
4-methoxyphenyl-[4-(4-methoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
disrupted microsomes
0.0033
-
4-methoxyphenyl-[4-(4-methoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
intact microsomes
0.00029
-
4-methoxyphenyl-[4-(4-trifluoromethoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
disrupted microsomes
0.00063
-
4-methoxyphenyl-[4-(4-trifluoromethoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
intact microsomes
0.00121
-
4-methoxyphenyl-[4-(4-trifluoromethoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
disrupted microsomes
0.004
-
4-methoxyphenyl-[4-(4-trifluoromethoxyphenyl)-4,5,6,7-tetrahydrothieno[3,2-c]pyridin-5-yl]methanone
-
intact microsomes
0.8
-
5-((((iodoacetyl)amino)ethyl)amino)naphtalene-1-sulfonic acid
-
-
-
0.00011
-
5-chlorotetrahydrothiophene-2-carbaldehyde dibenzylhydrazone
-
-
0.021
-
5-ethyltetrahydrofuran-2-carbaldehyde dibenzylhydrazone
-
-
0.00096
-
benzaldehyde dibenzylhydrazone
-
-
0.5
-
chlorogenic acid
-
-
50
200
D-glucose
-
-
0.237
-
nicotinaldehyde dibenzylhydrazone
-
-
2
-
nitrothiocyanobenzoic acid
-
-
-
0.0064
-
pyridine-2-carbaldehyde dibenzylhydrazone
-
-
0.000704
-
tetrahydrofuran-2-carbaldehyde dibenzylhydrazone
-
-
0.00096
-
tetrahydrothiophene-2-carbaldehyde dibenzylhydrazone
-
-
0.001
0.007
tungstate
-
in detergent treated microsomes
0.01
0.025
tungstate
-
in intact microsomes
0.0022
-
vanadate
-
in detergent treated microsomes
0.0056
-
vanadate
-
in intact microsomes
0.011
-
isonicotinaldehyde dibenzylhydrazone
-
-
additional information
-
additional information
-
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0885
-
1-[(4'-O-(E)-p-coumaroyl)-beta-D-glucopyranosyl]-oxy-2-phenol
-
63.7% inhibition at 0.1 mM
0.093
-
chlorogenic acid
-
69.6% inhibition at 0.1 mM
0.05
-
itoside H
-
85.4% inhibition at 0.1 mM
0.0935
-
orthovanadate
-
53.4% inhibition at 0.1 mM
0.095
-
pyrocatechol-O-beta-D-glucopyranoside
-
51.8% inhibition at 0.1 mM
0.051
-
quercetin-3-O-beta-D-glucopyranoside
-
82.9% inhibition at 0.1 mM
0.081
-
1-[(6'-O-(E)-p-coumaroyl)-beta-D-glucopyranosyl]-oxy-2-phenol
-
66.9% inhibition at 0.1 mM
additional information
-
1-[O-beta-D-glucopyranosyl(1->2)-beta-D-glucopyranosyl]-oxy-2-phenol
-
25.2% inhibition at 0.1 mM
additional information
-
3,4-dicaffeoylquinic acid
-
6.9% inhibition at 0.033 mM
additional information
-
4,5-dicaffeoylquinic acid
-
12.8% inhibition at 0.038 mM
additional information
-
4-caffeoylquinic acid
-
14.4% inhibition at 0.12 mM
additional information
-
5-caffeoylquinic acid
-
9.2% inhibition at 0.16 mM
additional information
-
apigenin-7-O-beta-D-glucopyranoside
-
42.7% inhibition at 0.1 mM
additional information
-
apigenin-7-O-beta-D-glucuronic acid-6''-methyl ester
-
37.2% inhibition at 0.1 mM
0.091
-
rutin
-
56.1% inhibition at 0.1 mM
additional information
-
Svetol
-
36% inhibition at 0.6 mM
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0078
-
P35575
purified FLAG-tagged recombinant mutant T16R
0.008
-
P35575
purified detagged recombinant mutant T16R
0.0083
-
P35575
purified detagged or FLAG-tagged recombinant mutant Y209C
0.011
-
-
brain
0.021
0.066
-
activity in hepatocellular carcinoma
0.047
-
-
arthritic rats
0.051
-
-
enzyme from liver
0.068
-
P35575
purified FLAG-tagged recombinant wild-type enzyme
0.095
-
-
control rats
0.095
-
-
liver microsomes
0.102
-
-
activity using pSVL expression system
0.114
0.239
-
activity in healthy normal liver
0.136
-
P35575
purified detagged recombinant wild-type enzyme
0.272
-
-
liver
0.335
-
-
enzyme from microsomes, at 25C
0.5473
-
-
enzyme from octylglucoside-solubilized extract, at 25C
1.065
-
-
enzyme from proteoliposomes dialyzed against MOPS buffer, at 25C
1.268
-
-
activity using adenoviral expression system
6.58
-
-
enzyme from placenta
47.5
-
-
intact microsome, presence of 0.1 mM epigallocatechin gallate
67.7
-
P35575
recombinant FLAG-tagged wild-type enzyme
80
-
-
intact microsome
107
-
-
permeabilized microsome, presence of 0.1 mM epigallocatechin gallate
124
-
-
permeabilized microsome
136
-
P35575
recombinant non-tagged wild-type enzyme
additional information
-
-
the activity increases up to the 20th month of rat life and then decreases rapidly
additional information
-
-
activity in woodchuck model of hepatitis virus-induced hepatocellular carcinoma
additional information
-
-
metabolic activity in different strains, overview
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
6
-
-
5.5
-
-
enzyme from testis
5.6
-
-
enzyme from pancreas
5.6
-
-
enzyme from brain
5.7
-
-
enzyme from adrenals
6
-
-
preparation without detergent
6
-
Q9BUM1
in disruptes vesicles
6
-
-
assay at
6.1
-
-
-
6.5
-
-
enzyme from placenta and liver
6.5
-
-
-
6.5
-
-
detergent-treated preparation
6.5
-
-
in disruptes vesicles
6.5
-
-
assay at
6.5
-
-
-
6.5
-
P35575
assay at
7.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
two enzyme forms: an acid-sensitive Glc-6-Pase-alpha in liver, kidney and intestine, and an acid-insensitive Glc-6-Pase-beta in astroctyes
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
P35575
assay at
37
-
-
assay at
37
-
-
-
37
-
-
assay at
37
-
-
assay at
additional information
-
Q98UF8, -
no effect of rearing water temperature on enzyme activity
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
Q98UF8, -
G6Pase activitiy and gene expression is not influenced by water temperature
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
8.5
-
Q9BUM1
-
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
low enzyme activities
Manually annotated by BRENDA team
-
equally distributed in all areas of the brain; the enzyme is found in all parts of the brain in the membraneous subcellular fractions of neurons
Manually annotated by BRENDA team
Q9BUM1
expression of G6PC3 isoform
Manually annotated by BRENDA team
-
contains a functional glucose-6-phosphatase complex capable of endogenous glucose production from glucose 6-phosphate, independent of blood glucose
Manually annotated by BRENDA team
Q9BUM1
expression of G6PC3 isoform
Manually annotated by BRENDA team
-
microsome; perifused hepatocyte
Manually annotated by BRENDA team
Q98UF8, -
juvenile fish
Manually annotated by BRENDA team
-
hepatitis virus-induced hepatocellular carcinoma
Manually annotated by BRENDA team
-
cortex, both mRNA and activity of enzyme appear during the first days of extratrauterine life, reaching a maximum after 2-3 weeks
Manually annotated by BRENDA team
Q9BUM1
expression of G6PC1 and G6PC3 isoforms
Manually annotated by BRENDA team
Frog
-
-
Manually annotated by BRENDA team
-
hepatoma cells, cell line MH1C1
Manually annotated by BRENDA team
-
enzyme activities greatly increases in rat liver around birth, the activities appears to be less specific in extracts of fetal liver enzyme than of adult liver
Manually annotated by BRENDA team
-
of arthritic and control rats in the fed and fast states
Manually annotated by BRENDA team
Q9BUM1
expression of G6PC1 isoform
Manually annotated by BRENDA team
-
enzyme activity in healthy and aged liver, activities in different liver regions; the woodchuck model of hepatocellular carcinoma has significantly decreased levels of G6Pase compared to healthy woodchuck. Significant differences in G6Pase activity between hepatocellular carcinoma liver region and surrounding normal liver region in the same woodchuck model of hepatocellular carcinoma
Manually annotated by BRENDA team
-
perifused hepatocyte
Manually annotated by BRENDA team
Q98UF8, -
; heaptic enzyme activity and gene expression are neither influenced by dietary carbohydrates nor water temperature
Manually annotated by BRENDA team
-
respiratory epithelium
Manually annotated by BRENDA team
Frog, salamander
-
pancreatic islets
Manually annotated by BRENDA team
Frog
-
-
Manually annotated by BRENDA team
-
syncytiotrophoblasts
Manually annotated by BRENDA team
Q9BUM1
expression of G6PC3 isoform
Manually annotated by BRENDA team
-
red portion and white portion
Manually annotated by BRENDA team
-
low enzyme activities
Manually annotated by BRENDA team
Q9BUM1
expression of G6PC3 isoform
Manually annotated by BRENDA team
additional information
-
the enzyme is expressed in beta-cells of pancreatic islets and in intestinal musoca, most particulary in the starved and diabetic states
Manually annotated by BRENDA team
additional information
-
expression of the enzyme and of glucose 6-phosphate transporter, overview, two enzyme forms: an acid-sensitive Glc-6-Pase-alpha in liver, kidney and intestine, and an acid-insensitive Glc-6-Pase-beta in astrocytes, overview
Manually annotated by BRENDA team
additional information
-
Ax4 cell, HDB7YA cell (smlA- cell), HDB2B/4 cell (cells with low counting factor)
Manually annotated by BRENDA team
additional information
-
IGRP13-25 and IGRP226-238 are DR0301-restricted epitopes, whereas IGRP23-35 and IGRP247-259 are DR0401-restricted epitopes
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
activity of glucose-6-phosphatase in crude cytosol is positively correlated with the amount of secreted multipolypeptide complex called counting factor
Manually annotated by BRENDA team
-
the enzyme is positioned on the inner aspect of the membrane of the endoplasmic reticulum
Manually annotated by BRENDA team
-
the enzyme is situated inside the lumen of the endoplasmic reticulum
Manually annotated by BRENDA team
-
the enzyme is anchored by nine transmembrane helices
Manually annotated by BRENDA team
-
enzyme is anchored by nine transmembrane helices
Manually annotated by BRENDA team
-
lumen, the enzyme-alpha and the enzyme-beta are anchored by nine transmembrane helices
Manually annotated by BRENDA team
-
membrane topology: a model with six transmembrane domains are first propsed, but has been superseded by a model with nine transmembrane domains
Manually annotated by BRENDA team
P35575
the enzyme contains several transmembrane helices
Manually annotated by BRENDA team
-
microsomal membrane
-
Manually annotated by BRENDA team
-
intact and disrupted
-
Manually annotated by BRENDA team
-
activity of glucose-6-phosphatase in crude microsome is negatively correlated with the amount of secreted multipolypeptide complex called counting factor
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
-
-
enzyme extracted with 10 mM Tris, pH 8.5, 1.0% sodium deoxycholate w/v, gel filtration
38730
-
Q9BUM1
calculation from sequence of cDNA
40000
-
-
Western blot analysis
41000
-
-
Western blot analysis
60800
-
-
gel filtration
70000
-
-
enzyme extracted with 10 mM sodium phosphate, pH 7.0, with 1.0% sodium deoxycholate and filtered on a Sephadex column equilibrated with the neutral buffer containing 0.1% detergent, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 36500, phosphohydrolase component of the microsomal glucose-6-phosphatase system
?
-
x * 17000, x * 18500, one or both of the heat-stable proteins appers to be glucose 6-phosphatase, SDS-PAGE
?
-
x * 28000, SDS-PAGE
?
Q98UF8
x * 39251, catalytic subunit, amino acid sequence calculation
?
Q19KA1
x * 40770, calculated
additional information
-
the phosphohydrolase is a component of the glucose-6-phosphatase system
additional information
-
the lack of a transport protein distinguishes the brain enzyme from the liver preparation
additional information
-
the enzyme is multiprotein system, the transmembrane catalytic subunit and the D-glucose 6-phosphate translocase have reactive thiol groups facing the cytosol
additional information
-
a 55000 Da protein is identified as D-glucose 6-phosphate translocase
additional information
-
a 50000 Da protein is identified as D-glucose 6-phosphate translocase
additional information
-
enzyme is multicomponent system, a 36000 Da enzyme catalytic subunit and a 46000 Da D-glucose 6-phosphate transporter component
additional information
Q9BUM1
structure comparison of G6PC1 and G6PC3 isoforms
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
glycoprotein
-
-
side-chain modification
-
enzyme is active as a phosphoprotein, dephosphorylation leads to inactivation
additional information
-
chemical modification of thiol groups belonging to proteins involved in the enzyme system by DTNB, NTCB and IAEDANS decrease the enzyme activity, the incubation with phosphate preventes the loss of activity, the addition of 2-mercaptoethanol allows a 50% restoration of the initial activity, however chemical modification of thiol groups by pHMPS, pHMB, AMSDS and NEM decrease the enzyme activity
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
-
-
stable
5
-
-
37C, 10 min, complete loss of activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
18
25
-
glucose-6-phosphatase activity is not influenced by water temperature (18C or 25C)
18
25
Q8AYI6
glucose-6-phosphatase activity is not influenced by water temperature (18C or 25C)
21
-
-
inactivated at room temperature
37
-
-
pH 5.0, 10 min, complete loss of activity
45
-
-
10 min, 80% loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
more than 60% loss of activity after freezing and thawing or incubation at room temperature for 15 min
-
substrate protects from inactivation by trypsin or chymotrypsin
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the native, intact microsomal membrane enzyme is not accessible to proteolytic attack from the cytoplasmic surface
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OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
The treatment with detergents, wheter anionic, cationic or neutral, modestly stimulates the hydrolysis of D-glucose 6-phosphate, but considerably increases the phosphatase activity on other substrates, such as mannose 6-phosphate
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649729
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, stable for a few months
-
-30C, not stable for more than a few days
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4C, stable for several hours
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial
-
recombinant FLAG-tagged wild-type enzyme and mutants Y209C and T16R from COS-1 cells, removal of the tag
P35575
the phosphate-enzyme intermediate
-
the phosphate-enzyme-beta intermediate
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
transient expression of the catalytic subunit in Hep-G2 cells
-
-
Q19KA1
cloning of two principal constituents: the catalytic subunit and D-glucose 6-phosphate translocase, expression studies in COS cells
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enzyme catalytic subunit and D-glucose 6-phosphate transporter component
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enzyme isoform of brain: G6PC3
Q9BUM1
expressed in COS-1 cells
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expressed in HEK-293 cells and Hep-G2 cells
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expressed in Hep-G2, HeLa, and CV1 cells
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expression of FLAG-tagged wild-type and mutant enzymes in COS-1 cells, the FLAG-tag reduces the activity of the recombinant wild-type enzyme compared to the non-tagged wild-type enzyme; expression of FLAG-tagged wild-type enzyme and mutants Y209C and T16R in COS-1 cells, very low expression level of mutant enzymes
P35575
cloning into an andoviral vector of nontagged or FLAG-tagged beta-subunit, expression in COS-1 cells
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expressed in rat H4IIE hepatoma cells
P35576
expressed in rat hepatocytes, HepG-2 cells, and H4IIE cells
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the expression of hepatic enzyme catalytic subunit in pancreatic islets is studied
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expression of the enzyme in H4IIE cells, determination of promoter activity and regulation, influences on enzyme expression by e.g. tunicamycin and thapsigargin, overview
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the expression of enzyme is transactivated by the transcription factor FKHR, DYRK1 is a specific co activator of FKHR
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DNA and amino acid sequence determination and analysis of the gene encoding the catalytic subunit, amino acid sequence alignment, expression of the catalytic subunit
Q98UF8
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression of hepatic glucose-6-phosphatase-alpha is down-regulated in patients With hepatic steatosis
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2 h after metformin treatment
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D38V
-
the mutation leads to reduced levels of G6PC protein
E110Q
-
the mutation retains 16% of wild type activity
F322L
-
mutant has similar stability to wild type enzyme
F327del/V338F/I341N/L345R
-
the mutation leads to reduced levels of G6PC protein
G122D
-
mutant has similar stability to wild type enzyme
G188D/G188S/G188R
-
the mutation leads to reduced levels of G6PC protein
G222R
-
the mutation retains 2.6% of wild type activity
G350X
-
the mutation retains 41.5% of wild type activity
H119A
-
mutation inactivates the phosphatase
H119L
-
the mutation completely abolishes enzymatic activity
H176A
-
mutation inactivates the phosphatase
H176A
-
mutant is devoid of D-glucose 6-phosphate hydrolase activity
H179A
-
mutation completely abolishes the enzyme activity
H197T
-
no significant change of enzyme activity
H252A
-
no significant change of enzyme activity
H307A
-
no significant change of enzyme activity
H353A
-
no significant change of enzyme activity
H353X
-
the mutation retains 60% of wild type activity
H52A
-
no significant change of enzyme activity
H9A
-
no significant change of enzyme activity
K355X
-
the mutation retains 65% of wild type activity
K76N
-
mutation inactivates the phosphatase
K76N
-
the mutation completely abolishes enzymatic activity
N264K/L265P/G266V/G270V/G270R
-
the mutation leads to reduced levels of G6PC protein
P257L
-
the mutation retains 6.1% of wild type activity
Q347X
-
the mutation completely inactivates the G6PC enzyme
Q351X
-
the mutation retains 43% of wild type activity
Q54P
P35575
naturally occurring missense mutation, no phenotype; naturally occurring mutation of the enzyme from an argentinian glycogen storage disease type Ia patient
R170Q
-
mutation inactivates the phosphatase
R170Q
-
the mutation completely abolishes enzymatic activity
R170X
-
the mutation completely inactivates the G6PC enzyme
R295C/S298P
-
the mutation leads to reduced levels of G6PC protein
R83C
-
mutation inactivates the phosphatase
R83C
P35575
naturally occurring missense mutation, no phenotype; naturally occurring mutation of the enzyme from an argentinian glycogen storage disease type Ia patient
R83C
-
the mutation completely abolishes enzymatic activity
R83H
-
the mutation completely abolishes enzymatic activity
T16A
P35575
site-directed mutagenesis, 74-78% reduced activity compared to the wild-type enzyme
T16R
P35575
naturally occurring N-terminal mutation of the enzyme from an argentinian glycogen storage disease type Ia patient, site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme; the isolated glucose-6-phosphatase mutation, in the sixth transmembrane helix, affects protein stability and causes glycogen storgae disease type Ia determined in Argentina, overview, the mutant constructed using site-sirected mutagenesis shows no enzymatic activity and reduced enzyme stability
V166A
-
the mutation leads to reduced levels of G6PC protein
W63R/G68R
-
the mutation leads to reduced levels of G6PC protein
Y209C
P35575
naturally occurring mutation in the sixth transmembrane helix of the enzyme from an argentinian glycogen storage disease type Ia patient, site-directed mutagenesis, about 90% reduced activity compared to the wild-type enzyme; the isolated glucose-6-phosphatase mutation, in the sixth transmembrane helix, affects protein stability and causes glycogen storgae disease type Ia determined in Argentina, overview, the mutant constructed using site-sirected mutagenesis shows no enzymatic activity and reduced enzyme stability
Y209C/L211P/G222R
-
the mutation leads to reduced levels of G6PC protein
L349X
-
the mutation retains 5.3% of wild type activity
additional information
-
structure and function studies of the 48 missence mutations and the F327 codon deletion mutation: the 5 active site mutations and 22 of 31 helical mutations completely abolish enzyme activity, but only 5 of the 13 nonhelical mutants are devoid of activity, a data base of residual enzymatic activity retained by enzyme mutants is obtained
additional information
P35575
mutational analysis of the genes encoding the enzyme from 11 argentinian patients suffering from glycogen storage disease type Ia, overview
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
crucial enzyme involved in glucose homeostasis
medicine
-
the enzyme deficiency is responsible for glycogen storage disease type I, starvation and diabetes cause a 2-3fold increase in enzyme activity in the liver
medicine
-
the enzyme catalytic unit is a therapeutic target for the treatment of type 2 diabetes, and the putative D-glucose 6-phosphate transporter component of the system has become a therapeutic target as well
medicine
-
deficiency in enzyme causes glycogen storage disease type 1, and s large number of enzyme mutations is identified in glycogen storage disease type 1a patients
medicine
-
deficiency in enzyme causes glycogen storage disease type 1a
medicine
-
IGRP is a member of the glucose-6-phosphatase family of proteins and the major autoantigen for autoimmune type 1 diabetes. DR0301-restricted IGRP-specific T cells present in 100% of healthy subjects and type 1 diabetes subjects. DR0401-restricted IGRP-specific T cells present in ca. 80% of the subjects. Cross-reactivity between IGRP and G6Pase-alpha epitopes for DR0401-restricted T cells; islet-specific glucose-6-phosphatase catalytic subunit-related protein is recognized as a major autoantigen for autoimmune type 1 diabetes. More than 80% of subjects with either DRB1*0401 or DRB1*0301 haplotype have islet-specific glucose-6-phosphatase catalytic subunit-related protein-specific CD4+ T cell responses for at least one islet-specific glucose-6-phosphatase catalytic subunit-related protein epitope. Islet-specific glucose-6-phosphatase catalytic subunit-related protein-specific T cells from both healthy and type 1 diabetes groups produce both gamma-interferon and interleukin-10
medicine
-
increased hexokinase activity and decreased glucose-6-phosphatase activity in the liver of the woodchuck model of hepatitis virus-induced hepatocellular carcinoma
medicine
-
arthritic rats present reduced activities of the hepatic enzyme
medicine
-
inhibitory effect of silibinin on both hepatic glucose-6-phosphatase and gluconeogenesis may be of interest in treatment of type 2 diabetes
medicine
-
inhibitors of liver enzyme involved in either glycogenolysis or glyconeogenesis ara potential targets for development of new drugs as alternatives to the existing treatments of type 2 diabetes