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Information on EC 3.1.3.8 - 3-phytase and Organism(s) Thermothelomyces thermophilus and UniProt Accession O00107
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3.1.3.8 3-phytaseSpecify your search results
Word Map
- 3.1.3.8
- phosphorus
- meal
-
broiler
- ash
- phytases
-
corn-soybean
- agriculture
- synthesis
-
phytic
-
barrows
- tibia
-
allotted
-
housed
-
meal-based
-
digesta
-
quadratic
-
nonphytate
-
phytase-supplemented
-
equivalency
-
growing-finishing
- nutrition
-
monogastric
-
excreta
-
indigestible
- ficuum
-
dicalcium
-
chromic
-
manure
-
p-deficient
-
corn-based
-
gain:feed
-
phytate-p
-
grower
-
crates
-
maize-soybean
-
low-phosphorus
-
t-cannulas
- 6-phytase
-
phosphorus-deficient
-
monocalcium
-
phytate-degrading
- food industry
-
as-fed
- biotechnology
-
feedstuffs
-
cornstarch
-
wheat-based
- analysis
-
metabolisable
The taxonomic range for the selected organisms is: Thermothelomyces thermophilus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Reaction Schemes
Synonyms
microbial phytase, natuphos, alkaline phytase, 3-phytase, phytase a, beta-propeller phytase, atpap15, cell-bound phytase, appa2, acid phytase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1-phytase
-
-
-
-
MYO-inositol-hexaphosphate 3-phosphohydrolase
-
-
-
-
pH 2.5 optimum acid phosphatase
-
-
-
-
PhyA
phytase
-
-
-
-
phytate 1-phosphatase
-
-
-
-
Phytate 3-phosphatase
-
-
-
-
phytate 6-phosphatase
-
-
-
-
St-Phy
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
myo-inositol-hexakisphosphate 3-phosphohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY
37288-11-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
i.e. phytase
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
-
-
?
myo-inositol hexakisphosphate + H2O
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
the enzyme is resistent against pepsin and trypsin, 95% of initial activity is retrieved after 1.0-h incubation in pepsin/trypsin. Poor inhibition by acetone, EDTA and 2-mercaptoethanol
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.143
myo-inositol hexakisphosphate
recombinant enzyme, pH 5.0, 60°C
0.156
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
0.156
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
additional information
additional information
thermodynamics
-
additional information
additional information
thermodynamics of the recombinant enzyme
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
37.8
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
5100
myo-inositol hexakisphosphate
recombinant enzyme, pH 5.0, 60°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
242.3
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
242.3
myo-inositol hexakisphosphate
native enzyme, pH 5.0, 60°C
37207.6
myo-inositol hexakisphosphate
recombinant enzyme, pH 5.0, 60°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
52.4
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
i.e. Sporotrichum thermophile or Thielavia heterothallica
UniProt
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
solid-state fermentation, optimization of enzyme phytase production by Sporotrichum thermophile, method, detailed overview. Maximum phytase production (1881.26 U/g dry mouldy residue) in 2.5% Tween 80, 1.0% yeast extract, and 48 h of incubation period. Phytase production in the mixed substrate (sugarcane bagasse and wheat bran) fermentation enhances 11.6fold over the initial production as a consequence of optimization. Phytase titres are sustainable in flasks, trays and column bioreactor (1796 to 2095 U/g dry mouldy residue), thus validating the model and the process for large-scale phytase production. When the yeast extract is replaced with corn steep liquor (2% w/v), a sustained enzyme titre (1890 U/g dry mouldy residue) is attained, making the process cost-effective. Among all the detergents, Tween 80 supports a higher phytase production than others. The enzyme efficiently liberates nutritional components from poultry feed (inorganic phosphate, soluble protein and reducing sugars) in a time-dependent manner
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PHYA_THETO
487
0
52538
Swiss-Prot
Secretory Pathway (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 53000, sequence calculation, x * 55000, recombinant His-tagged enzyme, SDS-PAGE, x * 90000, native enzyme, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60
80
60
purified native enzyme, pH 5.0, has a half-life of 16 h
60
purified recombinant His-tagged enzyme, pH 5.0, has a half-life of 4 h. Purified native enzyme, pH 5.0, has a half-life of 16 h
80
purified native enzyme, pH 5.0, has a half-life of 1.5 h
80
purified recombinant His-tagged enzyme, pH 5.0, has a half-life of 38 min. Purified native enzyme, pH 5.0, has a half-life of 1.5 h
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant refolded His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant refolded His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene MYCTH_2306203, construction of rSt-Phy gene using overlap extension PCR for removal of an intron, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha
gene PHYA, construction of rSt-Phy gene using overlap extension PCR for removal of an intron, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli inclusion bodies is solubilized by 6 M urea and 5 mM 2-mercaptoethanol in 100 mM Tris-HCl, pH 7.0. The cell-free lysate is added to the equilibrated resins and is kept for 1 h on a rocker. The recombinant protein bound to the column is refolded on the column by passing five column volumes of buffer A (6 M urea and 5 mM 2-mercaptoethanol in 100 mM Tris-HCl, pH 7.0) and buffer B (20% v/v glycerol and 5 mM 2-mercaptoethanol in 100 mM Tris-HCl, pH 7.0) in a linear gradient of 6.0 to 0.0 M urea
recombinant enzyme from Escherichia coli inclusion bodies is solubilized by 8 M urea
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
food industry
the recombinant enzyme rSt-Phy is useful in dephytinization of tandoori and naan (unleavened flat Indian breads), and bread, liberating soluble inorganic phosphate that mitigates anti-nutrient effects of phytic acid
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ranjan, B.; Singh, B.; Satyanarayana, T.
Characteristics of recombinant phytase (rSt-Phy) of the thermophilic mold Sporotrichum thermophile and its applicability in dephytinizing foods
Appl. Biochem. Biotechnol.
177
1753-1766
2015
Thermothelomyces thermophilus (O00107), Thermothelomyces thermophilus ATCC 42464 (O00107), Thermothelomyces thermophilus BCRC 31852 (O00107), Thermothelomyces thermophilus DSM 1799 (O00107)
Kumari, A.; Satyanarayana, T.; Singh, B.
Mixed substrate fermentation for enhanced phytase production by thermophilic mould Sporotrichum thermophile and its application in beneficiation of poultry feed
Appl. Biochem. Biotechnol.
178
197-210
2016
Thermothelomyces thermophilus (V5M269), Thermothelomyces thermophilus BJTLR50 (V5M269)
Singh, J.; Joshi, M.C.; Bhatnagar, R.
Cloning and expression of mycobacterial glutamine synthetase gene in Escherichia coli
Biochem. Biophys. Res. Commun.
30
634-638
2004
Thermothelomyces thermophilus (G2QH47), Thermothelomyces thermophilus ATCC 42464 (G2QH47), Thermothelomyces thermophilus BCRC 31852 (G2QH47), Thermothelomyces thermophilus DSM 1799 (G2QH47)
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