Information on EC 3.1.3.75 - phosphoethanolamine/phosphocholine phosphatase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FZ62

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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9FZ62


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria


The taxonomic range for the selected organisms is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
3.1.3.75
-
RECOMMENDED NAME
GeneOntology No.
phosphoethanolamine/phosphocholine phosphatase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
choline biosynthesis I
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Glycerophospholipid metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
phosphoethanolamine phosphohydrolase
Requires active site Mg2+ but also works, to a lesser extent, with Co2+ and Mn2+. The enzyme is highly specific for phosphoethanolamine and phosphocholine.
CAS REGISTRY NUMBER
COMMENTARY hide
52227-92-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphocholine + H2O
choline + phosphate
show the reaction diagram
-
-
-
?
phosphoethanolamine + H2O
ethanolamine + phosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphocholine + H2O
choline + phosphate
show the reaction diagram
Q9FZ62
-
-
-
?
phosphoethanolamine + H2O
ethanolamine + phosphate
show the reaction diagram
Q9FZ62
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
divalent metal ions required, highest activity with 1.5 mM Mg2+
Fe2+
divalent metal ions required, highest activity with 1.5 mM Mg2+
Mg2+
divalent metal ions required, highest activity with 1.5 mM Mg2+
Ni2+
divalent metal ions required, highest activity with 1.5 mM Mg2+
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.44
phosphocholine
50 mM Hepes/NaOH buffer, pH 7.0, 37C
1.16
Phosphoethanolamine
50 mM Hepes/NaOH buffer, pH 7.0, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.04
phosphocholine
50 mM Hepes/NaOH buffer, pH 7.0, 37C
13
Phosphoethanolamine
50 mM Hepes/NaOH buffer, pH 7.0, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.34
phosphocholine
50 mM Hepes/NaOH buffer, pH 7.0, 37C
1.06
Phosphoethanolamine
50 mM Hepes/NaOH buffer, pH 7.0, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.48
substrate phosphocholine, 50 mM Hepes/NaOH buffer, pH 7.0, 37C
3.67
substrate phosphoethanolamine, 50 mM Hepes/NaOH buffer, pH 7.0, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
theoretical value
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31570
calculated from amino acid sequence
32000
gel filtration, SDS-PAGE
64000
native gel elelctrophoresis, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 32000, monomer-dimer equilibrium, both the monomer and the dimer are active
monomer
1 * 32000, monomer-dimer equilibrium, both the monomer and the dimer are active
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-Sepharose activated column
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21