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Information on EC 3.1.3.70 - mannosyl-3-phosphoglycerate phosphatase and Organism(s) Pyrococcus horikoshii and UniProt Accession O58690

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.70 mannosyl-3-phosphoglycerate phosphatase
IUBMB Comments
Requires Mg2+. The enzyme from Pyrococcus horikoshii is specific for alpha-D-mannosyl-3-phosphoglycerate and forms part of the pathway for the synthesis of mannosylglycerate.
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Pyrococcus horikoshii
UNIPROT: O58690
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Word Map
The taxonomic range for the selected organisms is: Pyrococcus horikoshii
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
mpgp, mannosyl-3-phosphoglycerate phosphatase, mpg phosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
mannosyl-3-phosphoglycerate phosphatase
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-
-
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MPG phosphatase
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-
-
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MPGP
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-
-
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phosphatase, mannosyl-3-phosphoglycerate
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphate bond
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-
-
-
PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-mannosyl-3-phosphoglycerate phosphohydrolase
Requires Mg2+. The enzyme from Pyrococcus horikoshii is specific for alpha-D-mannosyl-3-phosphoglycerate and forms part of the pathway for the synthesis of mannosylglycerate.
CAS REGISTRY NUMBER
COMMENTARY hide
393512-74-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-(alpha-D-glucosyl)-3-phosphoglycerate + H2O
2-(alpha-D-mannosyl)-D-glycerate + phosphate
show the reaction diagram
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-
-
ir
2(alpha-D-mannosyl)-3-phosphoglycerate + H2O
2(alpha-D-mannosyl)-D-glycerate + phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2(alpha-D-mannosyl)-3-phosphoglycerate + H2O
2(alpha-D-mannosyl)-D-glycerate + phosphate
show the reaction diagram
-
enzyme is involved in biosynthesis of alpha-mannosylglycerate
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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activity without Mg2+ is 58% of the activity with 15 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.134
2(alpha-D-mannosyl)-3-phosphoglycerate
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 7.5
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pH 4.2: 60% of maximal actuvity, pH 7.5: about 45% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70 - 108
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70°C: about 45% of maximal activity, 108°C: about 20% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
apo enzyme form lacking magnesium ions using the multiple-wavelength anomalous diffraction method and magnesium-bound holo enzyme form using the molecular-replacement method, with 16-20% (w/v) PEG 3350, 0.2 M ammonium chloride and 0.1 M MES-NaOH pH 6.0
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
98
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half-life: 15.6 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, HiTrap Ni2+-chelating column chromatography, Source 15Q column chromatography, and Superdex 75 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Empadinhas, N.; Marugg, J.D.; Borges, N.; Santos, H.; Da Costa, M.S.
Pathway for the synthesis of mannosylglycerate in the hyperthermophilic archaeon Pyrococcus horikoshii. Biochemical and genetic characterization of key enzymes
J. Biol. Chem.
276
43580-43588
2001
Pyrococcus horikoshii
Manually annotated by BRENDA team
Kawamura, T.; Watanabe, N.; Tanaka, I.
Structure of mannosyl-3-phosphoglycerate phosphatase from Pyrococcus horikoshii
Acta Crystallogr. Sect. D
64
1267-1276
2008
Pyrococcus horikoshii (O58690), Pyrococcus horikoshii
Manually annotated by BRENDA team