Information on EC 3.1.3.66 - phosphatidylinositol-3,4-bisphosphate 4-phosphatase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
3.1.3.66
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RECOMMENDED NAME
GeneOntology No.
phosphatidylinositol-3,4-bisphosphate 4-phosphatase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-phosphatidyl-myo-inositol 3,4-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
show the reaction diagram
Mg2+-independent, the enzyme still works when the 2,3-bis(acyloxy)propyl group is removed, i.e., it hydrolyses Ins(1,3,4)P3 to Ins(1,3)P2, it also converts Ins(3,4)P2 to Ins-3-P
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-phosphoinositide degradation
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Inositol phosphate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
1-phosphatidyl-1D-myo-inositol-3,4-bisphosphate 4-phosphohydrolase
Mg2+-independent. This enzyme still works when the 2,3-bis(acyloxy)propyl group is removed, i.e., it hydrolyses Ins(1,3,4)P3 to Ins(1,3)P2. It also converts Ins(3,4)P2 into Ins-3-P.
CAS REGISTRY NUMBER
COMMENTARY hide
122653-78-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
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INPP4B is regulated by androgens at the level of transcription. INPP4B mRNA expression is induced in both LNCaP and VCaP AR-expressing prostate cancer cells. INPP4B and PTEN expression are correlated with the recurrence rate of patients with high and low expression levels of the proliferation marker Ki67
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
show the reaction diagram
1-phosphatidyl-myo-inositol 3,4-bisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
show the reaction diagram
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?
D-myo-inositol 1,3,4-trisphosphate + H2O
D-myo-inositol 3,4-bisphosphate + phosphate
show the reaction diagram
D-myo-inositol 3,4-bisphosphate + H2O
D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
D-myo-phosphatidylinositol-3,4-bisphosphate + H2O
D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
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prefered substrate
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phosphatidylinositol-4-phosphate + H2O
?
show the reaction diagram
prefered substrate
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
show the reaction diagram
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?
1-phosphatidyl-myo-inositol 3,4-bisphosphate + H2O
1-phosphatidyl-1D-myo-inositol 3-phosphate + phosphate
show the reaction diagram
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?
D-myo-inositol 3,4-bisphosphate + H2O
D-myo-inositol 4-phosphate + phosphate
show the reaction diagram
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?
additional information
?
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calpain-mediated inhibition of enzyme is involved in the phosphatidylinositol 3,4-bisphosphate accumulation in thrombin-stimulated platelets
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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no Mg2+ requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
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5,5'-dithiobis(nitrobenzoic acid)
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Inositol hexakisphosphate
KCl
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moderate salt concentrations
KNO3
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additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034 - 0.046
D-myo-Inositol 1,3,4-trisphosphate
0.028 - 0.039
D-myo-Inositol 3,4-bisphosphate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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primary, the enzyme is expressed in nonproliferative estrogen receptor-positive cells in the normal breast, and in estrogen receptor-positive, but not negative, breast cancer cell lines
Manually annotated by BRENDA team
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restricted to the Purkinje cell soma and their dendrites in the molecular layer
Manually annotated by BRENDA team
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recombinant enzyme
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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early and recycling endosome
Manually annotated by BRENDA team
RHD4 is selectively recruited to RabA4-blabeled membranes that are involved in polarized expansion of root hair cells. In conjunction with the phosphoinositide kinase PI-4Kb1, RHD4 regulates the accumulation of phosphatidylinositol-4-phosphate on membrane compartments at the tips of growing root hairs
Manually annotated by BRENDA team
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and endosome, after growth factor stimulation
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
full-length 4-ptase-1 cDNA subcloned into the EcoRI site of pWzl-Hygromycin vector
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pLXSN-Flag-INPP4B expressed in 293T cells or in SUM149 cells subcutaneously injected into nude mice
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wild-type RHD4 and the mutant rhd4-2 N-terminal catalytic domains expressed from Escherichia coli Rosetta cells or BL21 cells as GST fusion proteins. EYFP-wild-type fusion protein transformed into Arabidopsis under control of the native RHD4 promoter
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of INPP4A is uniform across the cerebellum and the Purkinje cells
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knockdown of INPP4B expression by shRNA
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level of 4-ptase-1 protein expressed in reconstituted embryonic fibroblasts reveals a 5fold increase relative to endogenous 4-ptase-1 in +/+MEFs
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optimal induction of INPP4B by an androgen receptor required the expression of the transcriptional coactivator NCoR
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Tgkd allele is associated with a decrease in Inpp4b expression
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G449S
rhd4-2 mutant containing a mutation in motif VII of the SAC phosphatase domain, reduces the phosphatidylinositol-4-phosphate phosphatase activity to residual levels
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine