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Information on EC 3.1.3.34 - deoxynucleotide 3'-phosphatase and Organism(s) Mus musculus and UniProt Accession Q9JM14

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.34 deoxynucleotide 3'-phosphatase
IUBMB Comments
Also catalyses the selective removal of 3'-phosphate groups from DNA and oligodeoxyribonucleotides. Induced in Escherichia coli by T-even phages.
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This record set is specific for:
Mus musculus
UNIPROT: Q9JM14
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The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
3'-deoxynucleotidase, cdN, mdN, nucleotidase, 3'-deoxyribo, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'-deoxynucleotidase
-
-
-
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nucleotidase, 3'-deoxyribo
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
deoxyribonucleotide 3'-phosphohydrolase
Also catalyses the selective removal of 3'-phosphate groups from DNA and oligodeoxyribonucleotides. Induced in Escherichia coli by T-even phages.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-18-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-dGMP + H2O
2'-deoxyguanosine + phosphate
show the reaction diagram
-
-
-
?
3'-dUMP + H2O
2'-deoxyguanosine + phosphate
show the reaction diagram
-
-
-
?
deoxynucleoside 3'-phosphate + H2O
deoxynucleoside + phosphate
show the reaction diagram
-
-
-
-
?
dTMP + H2O
thymidine + phosphate
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
deoxynucleoside 3'-phosphate + H2O
deoxynucleoside + phosphate
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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required for maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NT5C_MOUSE
200
0
23076
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by hanging drop vapor diffusion method, D12N mutant in complex with 3'-dUMP to 1.9 A resolution, space group P62, and in complex with 3'-dGMP to 2.0 A resolution, space group P62
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D12N
inactive
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
D12N mutant expressed in the Escherichia coli Rosetta strain
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
introduction of substituents in the 3'-position may result in nucleoside analogues with increased resistance to dephosphorylation, design of drugs that are less prone to inactivation by the deoxyribonucleotidases
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Magnusson, G.
Deoxyribonucleotide phosphatase from rat liver and cultured mouse fibroblasts
Eur. J. Biochem.
20
225-230
1971
Mus musculus, Rattus norvegicus
Manually annotated by BRENDA team
Wallden, K.; Rinaldo-Matthis, A.; Ruzzenente, B.; Rampazzo, C.; Bianchi, V.; Nordlund, P.
Crystal structures of human and murine deoxyribonucleotidases: Insights into recognition of substrates and nucleotide analogues
Biochemistry
46
13809-13818
2007
Homo sapiens, Mus musculus (Q9JM14)
Manually annotated by BRENDA team