Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.1.3.25 - inositol-phosphate phosphatase and Organism(s) Homo sapiens

for references in articles please use BRENDA:EC3.1.3.25
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.3 Phosphoric-monoester hydrolases
                3.1.3.25 inositol-phosphate phosphatase
IUBMB Comments
Acts on five of the six isomers of myo-inositol phosphate, all except myo-inositol 2-phosphate, but does not act on myo-inositol bearing more than one phosphate group. It also acts on adenosine 2'-phosphate (but not the 3'- or 5'- phosphates), sn-glycerol 3-phosphate and glycerol 2-phosphate. Two isoforms are known .
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
inositol monophosphatase, impase, inositol phosphatase, impa2, impa1, myo-inositol monophosphatase, inpp5f, impp, myo-inositol-1-phosphatase, inositol-1-phosphatase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I-1-Pase
-
-
-
-
IMP
-
-
-
-
IMPase
inositol 1-phosphate phosphatase
-
-
-
-
inositol monophosphatase
inositol monophosphate phosphatase
-
-
-
-
inositol phosphatase
inositol phosphohydrolase
-
-
-
-
inositol-1(or 4)-monophosphatase
-
-
-
-
inositol-1-phosphatase
-
-
-
-
L-myo-inositol-1-phosphate phosphatase
-
-
-
-
Lithium-sensitive myo-inositol monophosphatase A1
-
-
-
-
myo-inositol 1 (or 4) -monophosphatase
-
-
-
-
myo-inositol 1-phosphatase
-
-
-
-
myo-inositol monophosphatase
-
-
myo-inositol monophosphatase 2
-
-
Myo-inositol monophosphatase A2
-
-
-
-
myo-inositol-1(or 4)-phosphate phosphohydrolase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
myo-inositol-phosphate phosphohydrolase
Acts on five of the six isomers of myo-inositol phosphate, all except myo-inositol 2-phosphate, but does not act on myo-inositol bearing more than one phosphate group. It also acts on adenosine 2'-phosphate (but not the 3'- or 5'- phosphates), sn-glycerol 3-phosphate and glycerol 2-phosphate. Two isoforms are known [4].
CAS REGISTRY NUMBER
COMMENTARY hide
37184-63-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-galactose 1-phosphate + H2O
alpha-D-galactose + phosphate
show the reaction diagram
-
-
-
-
?
D-erythrose 4-phosphate + H2O
D-erythrose + phosphate
show the reaction diagram
-
-
-
-
?
D-fructose 1,6-bisphosphate + H2O
D-fructose 6-phosphate + phosphate
show the reaction diagram
-
-
-
-
?
D-fructose 1-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
-
-
-
?
D-fructose 6-phosphate + H2O
D-fructose + phosphate
show the reaction diagram
-
-
-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
show the reaction diagram
-
-
-
-
?
D-myo-inositol 1-phosphate + H2O
D-myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
show the reaction diagram
-
-
-
-
?
D-xylose 5-phosphate + H2O
D-xylose + phosphate
show the reaction diagram
-
-
-
-
?
DL-myo-inositol 1-phosphate + H2O
DL-myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
glycerol 2-phosphate + H2O
glycerol + phosphate
show the reaction diagram
-
-
-
-
?
inositol 4-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
the inositol ring docks to the enzyme by forming four hydrogen bonds: the 2-OH group with Asp104, the 3-OH with Asp231, the 5-OH group with Thr106, and the 6-OH with the carbonyl group of main chain of Gly205
-
-
?
L-myo-inositol 1-phosphate + H2O
L-myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 3-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 4-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 5-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
myo-inositol 6-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
myo-inositol 1-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 3-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 4-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 5-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
myo-inositol 6-phosphate + H2O
myo-inositol + phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme is involved in development of bipolar disorder
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
required
KCl
-
in its absence activation by Mg2+ occurres at 2fold lower concentrations
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2R,4R,6R)-2,4-dihydroxy-6-[4-(2-hydroxyphenyl)butoxy]cyclohexyl phosphate
-
-
(1S,2R,4S,6R)-6-(butylamino)cyclohexane-1,2,4-triol
-
-
(phenylmethanediyl)bis(phosphonic acid)
-
-
1-(4-hydroxyphenyloxy)ethane-1,1-bisphosphonic acid
2,3,7-trihydroxycyclohepta-2,4,6-trien-1-one
-
-
acetonitrile
-
-
Be2+
-
-
Dimethylsulfoxide
-
5% inhibition
L-690330
L-690488
-
a tetrapivaloyloxymethyl ester prodrug of L-690330
phosphate
-
-
additional information
-
high-throughput cell-based screening using scintillation proximity assay measuring G-protein-coupled receptor activation for evaluation of inhibitory potency of lithium mimetics, overview
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
calbindin D28k
-
-
calcium binding protein S100B
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12 - 0.46
DL-myo-inositol 1-phosphate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.3
(1S,2R,4S,6R)-6-(butylamino)cyclohexane-1,2,4-triol
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00007
(1R,2R,4R,6R)-2,4-dihydroxy-6-[4-(2-hydroxyphenyl)butoxy]cyclohexyl phosphate
Homo sapiens
-
-
0.0002
(phenylmethanediyl)bis(phosphonic acid)
Homo sapiens
-
-
0.0002
1-(4-hydroxyphenyloxy)ethane-1,1-bisphosphonic acid
Homo sapiens
-
-
0.0008
2,3,7-trihydroxycyclohepta-2,4,6-trien-1-one
Homo sapiens
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
scintillation proximity assay development
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.25
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
IMPA1_HUMAN
277
0
30189
Swiss-Prot
other Location (Reliability: 2)
SAC2_HUMAN
1132
0
128407
Swiss-Prot
other Location (Reliability: 3)
IMPA2_HUMAN
288
0
31321
Swiss-Prot
other Location (Reliability: 2)
E5RGY4_HUMAN
211
0
23294
TrEMBL
Mitochondrion (Reliability: 4)
H0YBL1_HUMAN
265
0
28640
TrEMBL
other Location (Reliability: 2)
A0A024R830_HUMAN
277
0
30189
TrEMBL
other Location (Reliability: 2)
K7ELF8_HUMAN
163
0
17865
TrEMBL
other Location (Reliability: 2)
E5RIP7_HUMAN
180
0
19925
TrEMBL
other Location (Reliability: 3)
K7EIH6_HUMAN
126
0
14210
TrEMBL
other Location (Reliability: 2)
E5RHE9_HUMAN
126
0
14194
TrEMBL
other Location (Reliability: 2)
A0A140VJL8_HUMAN
336
0
36695
TrEMBL
Mitochondrion (Reliability: 4)
CALB1_HUMAN
261
0
30025
Swiss-Prot
Mitochondrion (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
-
x * 30000, SDS-PAGE
32400
-
2 * 32400, about, sequence calculation
60000
-
SDS-PAGE
64900
-
IMPA2, analytical ultracentrifugation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
homodimer
-
-
additional information
-
IMPA2 structure analysis using IMPA1 crystal structure as a template, overview
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
compared of an in silico model of the IMPase-1/calbindin complex with the crystal structure of calcium binding protein S100B. Calbindin and S100B activate IMPase-1 in a similar mode
sitting drop vapor diffusion method, using 40% (w/v) PEG 3350, 0.2 M magnesium formate, at 22°C
sitting drop vapour diffusion method, at 20°C, with 10 mM potassium dihydrogen phosphate, 15% PEG 8000, 20% glycerol, and 2 mM MgSO4 for crystal 1, 10 mM potassium dihydrogen phosphate, 12% PEG 8000, 20% glycerol, and 2 mM MgSO4 for crystal 2, 25 mM potassium dihydrogen phosphate, 14% PEG 8000, and 5 mM LiCl for crystal 3, and 8 mM potassium dihydrogen phosphate, 14% PEG 8000, 20% glycerol, and 5 mM CaCl2 for crystal 4
-
structure in complex with inhibitor L-690330 and manganese ions, at 1.39 A resolution. Presence of L-690330 significantly increases the thermal stability of IMPase
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
5.4fold
-
HisTrap column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration
-
Q Sepharose column chromatography, Mono Q column chromatography, and Sephadex 75 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli BLR (DE3) pLysS cells
expression in Escherichia coli strain DH5alpha
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
-
the enzyme is a target for development of lithium-mimetic agents for the treatment of bipolar disorder, high-throughput cell-based screening using scintillation proximity assay for the discovery of enzyme inhibitors, overview
medicine
a homozygous variant consisting of a 5-bp duplication leads to a frameshift and a premature stop codon (p.Ser165Trpfs*10) that cosegregates with the disease of intellectual disability in 26 genotyped family members
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Creba, J.A.; Carey, F.; McCulloch, A.
Metabolism of inositol 1-phosphate in HL60 cells
Biochem. Soc. Trans.
16
557-558
1988
Homo sapiens
-
Manually annotated by BRENDA team
Huang, E.M.; Detwiler, T.C.
The effects of lithium on platelet phosphoinositide metabolism
Biochem. J.
236
895-901
1986
Homo sapiens
Manually annotated by BRENDA team
Conolly, T.M.; Wilson, D.B.; Bross, T.E.; Majerus, P.W.
Isolation and characterization of the inositol cyclic phosphate products of phosphoinositide cleavage by phospholipase C
J. Biol. Chem.
261
122-126
1986
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
McAllister, G.; Whiting, P.; Hammond, E.A.; Knowles, M.R.; Atack, J.R.; Bailey, F.J.; Maigetter, R.; Ragan, C.I.
cDNA cloning of human and rat brain myo-inositol monophosphatase. Expression and characterization of the human recombinant enzyme
Biochem. J.
284
749-754
1992
Bos taurus, Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Vincendon, P.; Corti, E.; Guindani, A.; Brunati, C.; Sponga, F.; Stefanelli, S.; Denaro, M.; Pelton, P.; Ganzhorn, A.; Islam, K.
An automated high volume assay to screen for inhibitors of myo-inositol monophosphatase from microbial fermentation broths
J. Antibiot.
49
710-712
1996
Homo sapiens
Manually annotated by BRENDA team
Atack, J.R.; Rapoport, S.I.; Varley, C.L.;
Characterization of inositol monophosphatase in human cerebrospinal fluid
Brain Res.
613
305-308
1993
Homo sapiens
Manually annotated by BRENDA team
Parthasarathy, L.; Vadnal, R.E.; Parthasarathy, R.; Shyamaldevi, C.S.
Minireview. Biochemical and molecular properties of lithium-sensitive myo-inositol monophosphatase
Life Sci.
54
1127-1142
1994
Bos taurus, Homo sapiens, Lilium longiflorum, Rattus norvegicus
Manually annotated by BRENDA team
Zheng, W.; Brandish, P.E.; Kolodin, D.G.; Scolnick, E.M.; Strulovici, B.
High-throughput cell-based screening using scintillation proximity assay for the discovery of inositol phosphatase inhibitors
J. Biomol. Screen.
9
132-140
2004
Homo sapiens
Manually annotated by BRENDA team
Miller, D.J.; Allemann, R.K.
myo-inositol monophosphatase: a challenging target for mood stabilising drugs
Mini Rev. Med. Chem.
7
107-113
2007
Homo sapiens
Manually annotated by BRENDA team
Arai, R.; Ito, K.; Ohnishi, T.; Ohba, H.; Akasaka, R.; Bessho, Y.; Hanawa-Suetsugu, K.; Yoshikawa, T.; Shirouzu, M.; Yokoyama, S.
Crystal structure of human myo-inositol monophosphatase 2, the product of the putative susceptibility gene for bipolar disorder, schizophrenia, and febrile seizures
Proteins
67
732-742
2007
Homo sapiens
Manually annotated by BRENDA team
Fujita, S.; Ohnishi, T.; Okuda, S.; Kobayashi, R.; Fukuno, S.; Furuta, D.; Kikuchi, T.; Yoshikawa, T.; Fujita, N.
In silico study on the substrate binding manner in human myo-inositol monophosphatase 2
J. Mol. Model.
17
2559-2567
2011
Homo sapiens
Manually annotated by BRENDA team
Singh, N.; Halliday, A.C.; Knight, M.; Lack, N.A.; Lowe, E.; Churchill, G.C.
Cloning, expression, purification, crystallization and X-ray analysis of inositol monophosphatase from Mus musculus and Homo sapiens
Acta Crystallogr. Sect. F
68
1149-1152
2012
Mus musculus (O55023), Mus musculus, Homo sapiens (P29218), Homo sapiens
Manually annotated by BRENDA team
Kraft, L.; Roe, S.M.; Gill, R.; Atack, J.R.
Co-crystallization of human inositol monophosphatase with the lithium mimetic L-690,330
Acta Crystallogr. Sect. D
74
973-978
2018
Homo sapiens (P29218), Homo sapiens
Manually annotated by BRENDA team
Agam, G.; Almog, O.
Calbindin D28k and S100B have a similar interaction site with the lithium-inhibitable enzyme inositol monophosphatase-1 a new drug target site
J. Med. Chem.
58
2042-2044
2015
Homo sapiens (P05937)
Manually annotated by BRENDA team
Figueiredo, T.; Melo, U.S.; Pessoa, A.L.; Nobrega, P.R.; Kitajima, J.P.; Rusch, H.; Vaz, F.; Lucato, L.T.; Zatz, M.; Kok, F.; Santos, S.
A homozygous loss-of-function mutation in inositol monophosphatase 1 (IMPA1) causes severe intellectual disability
Mol. Psychiatry
21
1125-1129
2016
Homo sapiens (P29218), Homo sapiens
Manually annotated by BRENDA team
Ferruz, N.; Tresadern, G.; Pineda-Lucena, A.; De Fabritiis, G.
Multibody cofactor and substrate molecular recognition in the myo-inositol monophosphatase enzyme
Sci. Rep.
6
30275
2016
Homo sapiens (P29218)
Manually annotated by BRENDA team