Any feedback?
Information on EC 3.1.3.2 - acid phosphatase and Organism(s) Solanum lycopersicum and UniProt Accession P83380
for references in articles please use BRENDA:EC3.1.3.2Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.1.3.2 acid phosphataseIUBMB Comments
Wide specificity. Also catalyses transphosphorylations.
Specify your search results
Word Map
- 3.1.3.2
- osteoclast
- lysosomal
- resorption
- marrow
-
cytochemical
- rankl
-
osteoclastogenesis
- cathepsin
-
multinucleated
- collagen
- osteoporosis
- granule
- beta-glucuronidase
- osteocalcin
- lymphocyte
- monocyte
-
prostate-specific
- osteoprotegerin
-
histochemistry
- periodontal
- metastases
- phosphorus
- phagocytic
- alveolar
-
trabecular
-
seminal
-
rankl-induced
- parathyroid
-
ovariectomized
- tibia
- femur
-
cytochemistry
-
lumbar
- osteolysis
- nfatc1
-
resorb
- tooth
- calcitonin
- m-csf
-
dentin
- calvaria
-
bone-specific
-
orthodontic
-
telopeptide
-
postmenopausal
-
histomorphometric
-
micro-ct
-
micro-computed
- medicine
- synthesis
- molecular biology
- phytase
- diagnostics
-
n-acetyl-beta-d-glucosaminidase
- biotechnology
- agriculture
The taxonomic range for the selected organisms is: Solanum lycopersicum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
acid phosphatase, tartrate-resistant acid phosphatase, prostatic acid phosphatase, tracp, acpase, uteroferrin, tracp 5b, phosphatidic acid phosphatase, tracp5b, pp2a phosphatase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acid monophosphatase
-
-
-
-
acid nucleoside diphosphate phosphatase
-
-
-
-
Acid phosphatase PII
-
-
-
-
acid phosphohydrolase
-
-
-
-
acid phosphomoesterase
-
-
-
-
acid phosphomonoester hydrolase
-
-
-
-
ACP1
-
-
-
-
AcPase
-
-
-
-
Adipocyte acid phosphatase, isozyme alpha
-
-
-
-
Adipocyte acid phosphatase, isozyme beta
-
-
-
-
APase
APASE6
-
-
-
-
glycerophosphatase
-
-
-
-
HPAP
-
-
-
-
IAP
intracellular acid phosphatase
LAP
-
-
-
-
Low molecular weight phosphotyrosine protein phosphatase
-
-
-
-
Minor phosphate-irrepressible acid phosphatase
-
-
-
-
NSAP
-
-
-
-
P56
-
-
-
-
P60
-
-
-
-
PAP
pH 2.5 acid phosphatase
-
-
-
-
pH 6-optimum acid phosphatase
-
-
-
-
phosphomonoesterase
-
-
-
-
purple acid phosphatase
SAP2
secreted acid phosphatase
Stationary-phase survival protein surE
-
-
-
-
Tartrate-resistant acid ATPase
-
-
-
-
TR-AP
-
-
-
-
TrATPase
-
-
-
-
uteroferrin
-
-
-
-
APase
-
-
-
-
PAP
-
-
-
-
PATHWAY SOURCE
PATHWAYS
BRENDA
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphate-monoester phosphohydrolase (acid optimum)
Wide specificity. Also catalyses transphosphorylations.
CAS REGISTRY NUMBER
COMMENTARY
9001-77-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-phosphoglycerate + H2O
glycerate + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
6-phospho-D-gluconate + H2O
D-gluconate + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
glycerol 3-phosphate + H2O
glycerol + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
phosphotyrosine + H2O
tyrosine + phosphate
-
reaction with SAP2, no activity with SAP1
-
?
1-naphthyl phosphate + H2O
1-naphthol + phosphate
-
reaction with SAP1 and SAP2
-
?
2-naphthyl phosphate + H2O
2-naphthol + phosphate
-
reaction with SAP1 and SAP2
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
reaction with SAP1 and SAP2
-
?
diphosphate + H2O
2 phosphate
-
reaction with SAP2, no activity with SAP1
-
?
phenyl phosphate + H2O
phenol + phosphate
-
reaction with SAP1 and SAP2
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
reaction with SAP1 and SAP2
-
?
additional information
?
-
-
enzyme also exhibits significant peroxidase activity, which is optimal at pH 9.0 and insensitive to Mg2+ or molybdate
-
?
additional information
?
-
-
SAP1 and SAP2 are multifunctional enzymes, the enzymes also exhibit significant peroxidase activity which is optimal at approximately pH 8.4 and insensitive to Mg2+ or molybdate
-
?
additional information
?
-
-
the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism
-
-
?
additional information
?
-
-
substrate specificity, overview, the enzyme shows broad substrate specificity and exhibits peroxidase activity, EC 1.11.1.7, optimal at pH 9.0 and insensitive to Mg2+ and molydate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
the enzyme scavenges phosphate from intracellular phosphate esters and might be involved in oxidative reagents metabolism
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
additional information
-
the enzyme is not affected by BaCl2, CaCl2, CoCl2, KCl, NaCl, or EDTA
Mg2+
-
5 mM, SAP1 is activated to 136% of control, SAP2 is activated to 181% of control
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
the purple enzyme is insensitive to L-tartrate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
phosphate starvation induces the enzyme activity 4fold
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
620
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.6 - 6.3
-
pH 2.6: about 50% of maximal activity, pH 6.3: about 65% of maximal activity, isoenzyme 1, hydrolysis of p-nitrophenyl phosphate
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). PPH1_SOLLC
9
0
1005
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
142000
57000
63000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
?
x * 50000, PAP1 exists as 50000 and 75000 isoforms, SDS-PAGE
?
x * 75000, PAP1 exists as 50000 and 75000 isoforms, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
65
70
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
intracellular enzyme 476fold to homogeneity from phosphate-starved cell culture by PEG 8000 treatment, adsorption chromatography, gel filtration, and concanavalin A affinity chromatography
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
phosphate deficiency increases intracellular and secretory enzyme activity in a concentration-dependent manner. The 50000 Da isoform PAP1 is induced after 2 days of phosphate starvation and the 75000 Da isoform is induced after 16 days of phosphate starvation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Paul, E.M.; Williamson, V.M.
Purification and properties of acid phosphatase-1 from a nematode resistant tomato cultivar
Plant Physiol.
84
399-403
1987
Solanum lycopersicum
Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
Eur. J. Biochem.
269
6278-6286
2002
Solanum lycopersicum
Schenk, G.; Korsinczky, M.L.J.; Hume, D.A.; Hamilton, S.; DeJersey, J.
Purple acid phosphatases from bacteria: similarities to mammalian and plant enzymes
Gene
255
419-424
2000
Solanum lycopersicum
Bozzo, G.G.; Raghothama, K.G.; Plaxton, W.C.
Structural and kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell cultures
Biochem. J.
377
419-428
2004
Solanum lycopersicum
Bozzo, G.G.; Dunn, E.L.; Plaxton, W.C.
Differential synthesis of phosphate-starvation inducible purple acid phosphatase isozymes in tomato (Lycopersicon esculentum) suspension cells and seedlings
Plant Cell Environ.
29
303-313
2006
Solanum lycopersicum (P83379), Solanum lycopersicum (P83380), Solanum lycopersicum
EXTERNAL LINKS (specific for EC-Number 3.1.3.2)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
