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EC Tree
The taxonomic range for the selected organisms is: Escherichia coli The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
acid phosphatase, tartrate-resistant acid phosphatase, prostatic acid phosphatase, tracp, acpase, uteroferrin, tracp 5b, phosphatidic acid phosphatase, tracp5b, pp2a phosphatase,
more
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acid monophosphatase
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acid nucleoside diphosphate phosphatase
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-
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Acid phosphatase PII
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acid phosphohydrolase
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-
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acid phosphomoesterase
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-
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acid phosphomonoester hydrolase
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-
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Adipocyte acid phosphatase, isozyme alpha
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-
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Adipocyte acid phosphatase, isozyme beta
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-
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glycerophosphatase
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Low molecular weight phosphotyrosine protein phosphatase
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-
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Minor phosphate-irrepressible acid phosphatase
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pH 2.5 acid phosphatase
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pH 6-optimum acid phosphatase
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phosphomonoesterase
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Stationary-phase survival protein surE
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Tartrate-resistant acid ATPase
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additional information
the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases
additional information
the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases
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a phosphate monoester + H2O = an alcohol + phosphate
a phosphate monoester + H2O = an alcohol + phosphate
detailed structure-based catalytic mechanism
a phosphate monoester + H2O = an alcohol + phosphate
modelling of substrate binding in the active site
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phosphate-monoester phosphohydrolase (acid optimum)
Wide specificity. Also catalyses transphosphorylations.
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3'-AMP + H2O
adenosine + phosphate
-
-
-
?
3'-CMP + H2O
cytidine + phosphate
-
-
-
?
3'-dAMP + H2O
3'-deoxyadenosine + phosphate
-
-
-
?
3'-dCMP + H2O
deoxycytidine + phosphate
best substrate
-
-
?
3'-dGMP + H2O
deoxyguanosine + phosphate
-
-
-
?
3'-dUMP + H2O
deoxyuridine + phosphate
-
-
-
?
3'-GMP + H2O
guanosine + phosphate
-
-
-
?
3'-UMP + H2O
uridine + phosphate
-
-
-
?
3-phosphoglycerate + H2O
glycerate + phosphate
low activity
-
-
?
4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
-
-
-
?
5'-AMP + H2O
adenosine + phosphate
-
-
-
?
5'-CMP + H2O
cytidine + phosphate
-
-
-
?
5'-dAMP + H2O
deoxyadenosine + phosphate
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-
-
?
5'-dCMP + H2O
deoxycytidine + phosphate
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-
-
?
5'-dGMP + H2O
deoxyguanosine + phosphate
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-
-
?
5'-dUMP + H2O
deoxyuridine + phosphate
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-
-
?
5'-GMP + H2O
guanosine + phosphate
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-
-
?
5'-UMP + H2O
uridine + phosphate
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-
-
?
ADP + H2O
AMP + phosphate
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-
-
?
AMP + H2O
adenosine + phosphate
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-
-
?
ATP + H2O
ADP + phosphate
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-
-
?
D-glucose 6-phosphate + H2O
D-glucose + phosphate
low activity
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-
?
D-ribose 5-phosphate + H2O
D-ribose + phosphate
low activity
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-
?
1-glycerate phosphate + H2O
glycerate + phosphate
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-
-
-
?
2,3-diphosphoglycerate + H2O
?
-
-
-
-
?
acetylphosphate + H2O
acetate + phosphate
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-
-
-
?
ADP + H2O
AMP + phosphate
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-
-
-
?
ATP + H2O
ADP + phosphate
D-fructose 1,6-diphosphate + H2O
?
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-
-
-
?
glucose 1-phosphate + H2O
glucose + phosphate
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-
-
-
?
Glucose 6-phosphate + H2O
Glucose + phosphate
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-
-
-
?
GTP + H2O
GDP + phosphate
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-
-
-
?
p-nitrophenyl phosphate + H2O
p-nitrophenol + phosphate
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-
-
-
?
phosphoenolpyruvate + H2O
pyruvate + phosphate
-
-
-
-
?
additional information
?
-
ATP + H2O
ADP + phosphate
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-
-
-
?
ATP + H2O
ADP + phosphate
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low activity
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-
?
additional information
?
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substrate specificity of AphA, overview
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?
additional information
?
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substrate specificity of AphA, overview
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?
additional information
?
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synthesis starts as soon as cultures enter the stationary phase, strong negative control by cAMP, starvation for inorganic phosphate results in a premature full induction
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?
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additional information
?
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synthesis starts as soon as cultures enter the stationary phase, strong negative control by cAMP, starvation for inorganic phosphate results in a premature full induction
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?
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Cl-
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stimulates at pH 3.6
Br-
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slight stimulation
Br-
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stimulates at pH 3.6
I-
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slight stimulation
I-
-
stimulation at pH 3.6
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1,10-phenanthroline
IC50 is 0.0020 mM
dipicolinic acid
IC50 is 0.0025 mM
ATP
-
competitive inhibitor of hydrolysis of p-nitrophenyl phosphate
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citrate
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-
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0.0013
3'-dCMP
pH 6.0, 37°C, recombinant AphA
1.8
p-nitrophenyl phosphate
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-
additional information
additional information
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additional information
additional information
steady-state kinetics
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additional information
additional information
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steady-state kinetics
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101
3'-dCMP
pH 6.0, 37°C, recombinant AphA
additional information
additional information
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additional information
additional information
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additional information
additional information
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0.002
1,10-phenanthroline
Escherichia coli
IC50 is 0.0020 mM
0.0025
dipicolinic acid
Escherichia coli
IC50 is 0.0025 mM
0.0004
EDTA
Escherichia coli
IC50 is 0.0004 mM
0.0025
EGTA
Escherichia coli
IC50 is 0.0025 mM
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additional information
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additional information
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-
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2.5
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gene aphA
SwissProt
brenda
gene aphA, strain MG1655
SwissProt
brenda
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brenda
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45000
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1 * 45000, SDS-PAGE
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monomer
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1 * 45000, SDS-PAGE
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purified recombinant AphA in complex with adenosine and phosphate, with dCMP, or with osmate, sitting drop method, 0.002 ml protein solution containing 10 mg/ml protein in sodium acetate buffer, pH 7.0, mixed with 0.1 ml of precipitant solution containing 17-22% w/v PEG 6000, and 1 mM MgCl2, 20°C, equilibration against 2 ml of precipitant solution, soaking in ligand solution containing 50 mM sodium acetate, pH 7.0, 35% w/v PEG 6000, and 50 mM ligand for ligand complexing, derivatizing with Au and spermine, X-ray diffraction structure determination and analysis at 1.25-2.14 A resolution
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-18°C, 1 year, no significant modification
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recombinant AphA from strain DH5alpha
recombinant AphA from strain DH5alpha
recombinant AphA from strain DH5alpha
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gene aphA, overexpression in strain DH5alpha
expression in Solanum tuberosum under control of sweet potato sporamin promoter
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gene aphA, overexpression in strain DH5alpha
gene aphA, overexpression in strain DH5alpha
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agriculture
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transgenic expression of phytase in Solanum tuberosum leads to stable expression levels over several cycles of propagation. Field tests show that tuber size, number and yield increase in transgenic potato. Improved phosphorus acquisition when phytate is provided as a sole phosphorus source and enhanced microtuber formation in cultured transgenic potato seedlings when phytate is provided as an additional phosphorus source are observed. The potato-produced phytase supplement is as effective as a commercially available microbial phytase in increasing the availability of phytate-phosphorus to weanling pigs
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Touati, E.; Danchin, A.
Cloning and characterization of the pH 2.5 acid phosphatase gene, appA: cyclic AMP mediated negative regulation
Mol. Gen. Genet.
208
499-505
1987
Escherichia coli
brenda
Dassa, E.; Cahu, M.; Desjoyaux-Cherel, B.; Boquet, P.L.
The acid phosphatase with optimum pH of 2.5 of Escherichia coli. Physiological and biochemical study
J. Biol. Chem.
257
6669-6676
1982
Escherichia coli
brenda
Hollander, V.P.
Acid phosphatases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
4
449-498
1971
Bos taurus, Saccharomyces cerevisiae, Chaos carolinense, Drosophila melanogaster, Escherichia coli, Homo sapiens, Lupinus luteus, Staphylococcus aureus, Mus musculus, Neurospora crassa, Nicotiana tabacum, Rattus norvegicus, Saccharomyces mellis, Staphylococcus aureus PS 55
-
brenda
Hafkenscheid, J.C.M.
Properties of an acid phosphatase in Escherichia coli
Biochim. Biophys. Acta
167
582-589
1968
Escherichia coli
brenda
Passariello, C.; Forleo, C.; Micheli, V.; Schippa, S.; Leone, R.; Mangani, S.; Thaller, M.C.; Rossolini, G.M.
Biochemical characterization of the class B acid phosphatase (AphA) of Escherichia coli MG1655
Biochim. Biophys. Acta
1764
13-19
2006
Escherichia coli (P0AE22), Escherichia coli, Escherichia coli MG1655 (P0AE22)
brenda
Calderone, V.; Forleo, C.; Benvenuti, M.; Thaller, M.C.; Rossolini, G.M.; Mangani, S.
A structure-based proposal for the catalytic mechanism of the bacterial acid phosphatase AphA belonging to the DDDD superfamily of phosphohydrolases
J. Mol. Biol.
355
708-721
2006
Escherichia coli (P0AE22), Escherichia coli
brenda
Hong, Y.F.; Liu, C.Y.; Cheng, K.J.; Hour, A.L.; Chan, M.T.; Tseng, T.H.; Chen, K.Y.; Shaw, J.F.; Yu, S.M.
The sweet potato sporamin promoter confers high-level phytase expression and improves organic phosphorus acquisition and tuber yield of transgenic potato
Plant Mol. Biol.
67
347-361
2008
Escherichia coli
brenda