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Information on EC 3.1.3.12 - trehalose-phosphatase and Organism(s) Oryza sativa and UniProt Accession Q9FWQ2
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3.1.3.12 trehalose-phosphataseSpecify your search results
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
trehalose-6-phosphate phosphatase, attps1, ostpp1, gob-1, trehalose 6-phosphate phosphatase, otsb2, trehalose-6-phosphate synthase/phosphatase, trehalose phosphate phosphatase, trehalose-phosphate phosphatase, t6p phosphatase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
OsTPP1
TPP
trehalose 6-phosphatase
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trehalose 6-phosphate phosphatase
trehalose phosphate phosphatase
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TPP
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trehalose 6-phosphate phosphatase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of phosphoric ester
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PATHWAY SOURCE
PATHWAYS
BRENDA
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-, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
alpha,alpha-trehalose-6-phosphate phosphohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9025-72-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
trehalose 6-phosphate + H2O
trehalose + phosphate
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the enzyme is involved in trehalose biosynthesis, trehalose serves as a stress protectant and/or reserve carbohydrate
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?
additional information
?
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almost no activity with glucose 1-phosphate, glucose 6-phosphate, galactose 6-phosphate, mannose 1-phosphate, mannose 6-phosphate, fructose 1-phosphate, fructose 6-phosphate, sucrose 6-phosphate, lactose 1-phosphate, and ribose 5-phosphate
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-
?
additional information
?
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almost no activity with glucose 1-phosphate, glucose 6-phosphate, galactose 6-phosphate, mannose 1-phosphate, mannose 6-phosphate, fructose 1-phosphate, fructose 6-phosphate, sucrose 6-phosphate, lactose 1-phosphate, and ribose 5-phosphate
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
trehalose 6-phosphate + H2O
trehalose + phosphate
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the enzyme is involved in trehalose biosynthesis, trehalose serves as a stress protectant and/or reserve carbohydrate
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-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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enzyme induction by abscisic acid with in 20 min after application, chilling stress at 4°C or 12°C, transiently induces the enzyme in seedling roots and shoots, induction also by salt stress and by drought stress at ambient temperature
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0921
trehalose 6-phosphate
in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.03 - 0.55
trehalose 6-phosphate
in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C
6.52
trehalose 6-phosphate
in 50mM Tris /HCl buffer with 2mM MgCl2, at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
expression analysis of the OsTPP1 gene, initial and transient up-regulation after salt, osmotic and abscisic acid (ABA) treatments, slow up-regulation under cold stress, trehalose content determined by chromatography, overexpression of the OsTPP1 gene alters the expression of stress responsive genes and enhances tolerance to salt and cold stress, involvement in stress tolerance, possible use of OsTPP1 in abiotic stress engineering of crops
additional information
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expression analysis of the OsTPP1 gene, initial and transient up-regulation after salt, osmotic and abscisic acid (ABA) treatments, slow up-regulation under cold stress, trehalose content determined by chromatography, overexpression of the OsTPP1 gene alters the expression of stress responsive genes and enhances tolerance to salt and cold stress, involvement in stress tolerance, possible use of OsTPP1 in abiotic stress engineering of crops
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50
almost completely loses activity when subjected to heat treatment at 50°C for 4 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, pBluescript SK vector, pHB expression vector
gene TPP1, expression analysis in rice, low level expression under normal conditions, induction under chilling stress, transient expression in and functional complementation of a yeast enzyme-deficient mutant strain
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
possible use of OsTPP1 in abiotic stress engineering of crops
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pramanik, M.H.; Imai, R.
Functional identification of a trehalose 6-phosphate phosphatase gene that is involved in transient induction of trehalose biosynthesis during chilling stress in rice
Plant Mol. Biol.
58
751-762
2005
Oryza sativa
Shima, S.; Matsui, H.; Tahara, S.; Imai, R.
Biochemical characterization of rice trehalose-6-phosphate phosphatases supports distinctive functions of these plant enzymes
FEBS J.
274
1192-1201
2007
Oryza sativa (Q75WV3), Oryza sativa, Oryza sativa Japonica Group (Q9FWQ2)
Ge, L.F.; Chao, D.Y.; Shi, M.; Zhu, M.Z.; Gao, J.P.; Lin, H.X.
Overexpression of the trehalose-6-phosphate phosphatase gene OsTPP1 confers stress tolerance in rice and results in the activation of stress responsive genes
Planta
228
191-201
2008
Oryza sativa (Q75WV3), Oryza sativa
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