Information on EC 3.1.27.6 - Enterobacter ribonuclease

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The expected taxonomic range for this enzyme is: Enterobacter

EC NUMBER
COMMENTARY hide
3.1.27.6
-
RECOMMENDED NAME
GeneOntology No.
Enterobacter ribonuclease
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates
show the reaction diagram
endonucleolytic cleavage to nucleoside 3'-phosphate and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-99-4
not distinguished from EC 3.1.26.10
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
RNA + H2O
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
RNA + H2O
nucleoside 3'-phosphates and 3'-phosphooligonucleotides and 2',3'-cyclic phosphates
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
stimulating
Cd2+
-
reverses EDTA- and o-phenanthroline-induced inhibition
Mn2+
-
reverses EDTA- and o-phenanthroline-induced inhibition
Ni2+
-
inhibitory even at low concentrations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
inhibition after enzyme dialysis against 0.001 M EDTA
o-phenanthroline
-
inhibition after enzyme dialysis against 0.001 M o-phenanthroline
Poly(A)
Poly(G)
-
inhibition at a concentration of 0.25 nM is complete
additional information
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CdiA-CT toxins are specifically neutralized by cognate CdiI immunity proteins to protect toxin-producing cells from autoinhibition
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
putrescine
-
enhances activity against poly(C) and yeast RNA
spermidine
-
enhances activity against poly(C) and yeast RNA, reverses inhibition by poly(G) by removal of inhibitor from the enzymes surface
spermine
-
enhances activity against poly(C) and yeast RNA, reverses inhibition by poly(G) by removal of inhibitor from the enzymes surface
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
no activity below, poly(C)
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24300 - 25500
-
gel filtration, MW depending on purification procedure
28500 - 31500
-
sucrose density gradient centrifugation, MW depending on purification procedure
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
for 15 min, 100% activity
65
-
for 15 min, 78-80% activity depending on purification procedure
75
-
for 15 min, 53-57% activity depending on purification procedure
85
-
for 15 min, 18-20% activity depending on purification procedure
100
-
for 15 min, total loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, stable, withstands freezing and thawing
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-196°C, stable, withstands freezing and thawing
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-20°C, 0.1 mg protein per ml Tris-HCL elution buffer, stable for about 3 weeks
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-20°C, lyophilized enzyme, stable for about 3 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cloning of cdiA-CTECL under the control of an arabinose-inducible promoter and expression in Escherichia coli, which leads to growth inhibition of the bacterial cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Show AA Sequence (283 entries)
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