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Information on EC 3.1.26.5 - ribonuclease P and Organism(s) Methanococcus maripaludis and UniProt Accession P62378

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.26 Endoribonucleases producing 5'-phosphomonoesters
                3.1.26.5 ribonuclease P
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This record set is specific for:
Methanococcus maripaludis
UNIPROT: P62378 not found.
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Word Map
The taxonomic range for the selected organisms is: Methanococcus maripaludis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor
Synonyms
rnase p, rnase p rna, rnase mrp, ribonuclease p, m1 rna, c5 protein, rnase p protein, rnase p holoenzyme, rpp30, protein c5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hPOP1
-
-
-
-
hPOP4
-
-
-
-
hPOP7
-
-
-
-
nuclease, ribo-, P
-
-
-
-
Protein C5
-
-
-
-
ribonuclease P
-
-
-
-
RNA processing protein POP1
-
-
-
-
RNA processing protein POP5
-
-
-
-
RNA processing protein POP6
-
-
-
-
RNA processing protein POP7
-
-
-
-
RNA processing protein POP8
-
-
-
-
RNase P
RNase P protein
-
-
-
-
RNaseP protein
-
-
-
-
RNaseP protein p20
-
-
-
-
RNaseP protein p30
-
-
-
-
RNaseP protein p38
-
-
-
-
RNaseP protein p40
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
71427-00-4
not distinguished from EC 3.1.26.7
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
pre-tRNA + H2O
tRNA + 5' leader of tRNA
show the reaction diagram
-
RNase P holoenzymes, reconstituted in vitro
-
-
?
pre-tRNATyr + H2O
tRNATyr + 5'-oligoribonucleotide
show the reaction diagram
-
-
-
?
additional information
?
-
-
an external guide sequence, EGS, RNA base-paired to a target RNA makes the latter a substrate for endogenous RNase P by rendering the bipartite target RNA-EGS complex a precursor tRNA structural mimic. RNase P holoenzymes recognize and cleave such substrate-EGS complexes. The external guide sequences engage in multiple rounds of substrate recognition while assisting archaeal RNase P-mediated cleavage of a target RNA in vitro
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000044 - 0.0026
pre-tRNATyr
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17 - 1.05
pre-tRNATyr
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
65.4 - 23860
pre-tRNATyr
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36 - 38
optimal assay temperature of in vitro reconstituted enzyme in the absence of ribosomal protein L7Ae
48 - 50
optimal assay temperature of in vitro reconstituted enzyme in the presence of ribosomal protein L7Ae
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A7J9S5Q2_METMI
232
0
26507
TrEMBL
-
A0A7J9NQ04_METMI
110
0
12903
TrEMBL
-
A0A7J9PK83_METMI
110
0
12994
TrEMBL
-
A0A7J9NH28_METMI
98
0
11296
TrEMBL
-
A0A7J9NZC7_METMI
232
0
26449
TrEMBL
-
A0A7J9P4D5_METMI
223
0
25686
TrEMBL
-
A0A2L1C861_METMI
130
0
15178
TrEMBL
-
A0A7J9NMV0_METMI
130
0
15206
TrEMBL
-
A0A7J9NW08_METMI
232
0
26466
TrEMBL
-
A0A7J9PDP3_METMI
232
0
26420
TrEMBL
-
A0A7J9NT35_METMI
223
0
25673
TrEMBL
-
A0A2L1CC69_METMI
98
0
11310
TrEMBL
-
A0A7J9PHK9_METMI
232
0
26448
TrEMBL
-
A0A2L1C835_METMI
110
0
12892
TrEMBL
-
A0A7J9RWQ4_METMI
130
0
15177
TrEMBL
-
A0A7J9NLC9_METMI
232
0
26506
TrEMBL
-
A0A7J9P9T8_METMI
98
0
11282
TrEMBL
-
A0A7J9NSN8_METMI
110
0
12891
TrEMBL
-
A0A7J9S0L4_METMI
232
0
26464
TrEMBL
-
A0A7J9S1X8_METMI
110
0
12936
TrEMBL
-
A0A7J9PS30_METMI
232
0
26491
TrEMBL
-
A0A7J9P6B6_METMI
232
0
26462
TrEMBL
-
A0A7J9PIU1_METMI
223
0
25831
TrEMBL
-
A0A7J9S8G7_METMI
130
0
15208
TrEMBL
-
A0A2L1CBK6_METMI
223
0
25659
TrEMBL
-
A0A7J9PGL8_METMI
130
0
15213
TrEMBL
-
A0A7J9PR83_METMI
98
0
11309
TrEMBL
-
A0A2L1C9F6_METMI
232
0
26480
TrEMBL
-
A0A7J9NIU6_METMI
232
0
26535
TrEMBL
-
A0A7J9PDQ2_METMI
98
0
11419
TrEMBL
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N32A/E33A/K36A
mutant protein L7Ae displays only 4% of the activity observed with the wild-type protein L7Ae
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
RNA-mediated RNA cleavage events are being increasingly exploited to disrupt RNA function, an important objective in post-genomic biology. RNase P, a ribonucleoprotein enzyme that catalyzes the removal of 5'-leaders from precursor tRNAs, has previously been utilized for sequence-specific cleavage of cellular RNAs
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cho, I.M.; Kazakov, S.A.; Gopalan, V.
Evidence for recycling of external guide sequences during cleavage of bipartite substrates in vitro by reconstituted archaeal RNase P
J. Mol. Biol.
405
1121-1127
2011
Methanothermobacter thermautotrophicus, Methanocaldococcus jannaschii, Methanococcus maripaludis, Pyrococcus furiosus
Manually annotated by BRENDA team
Cho, I.M.; Lai, L.B.; Susanti, D.; Mukhopadhyay, B.; Gopalan, V.
Ribosomal protein L7Ae is a subunit of archaeal RNase P
Proc. Natl. Acad. Sci. USA
107
14573-14578
2010
Methanococcus maripaludis (P62426 and P60780 and P60781 and P60832 and P62378), Methanococcus maripaludis
Manually annotated by BRENDA team