Information on EC 3.1.26.5 - ribonuclease P

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.1.26.5
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RECOMMENDED NAME
GeneOntology No.
ribonuclease P
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
tRNA processing
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CAS REGISTRY NUMBER
COMMENTARY hide
71427-00-4
not distinguished from EC 3.1.26.7
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain 1A40
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Manually annotated by BRENDA team
enzyme is a catalytically active RNA, at least 2 isoforms with different substrate specificities
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Manually annotated by BRENDA team
fragment
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Manually annotated by BRENDA team
calf
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Clostridium innocuum
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain MRE600
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
Methanothermobacter thermautotrophicum Marburg
strain Marburg
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
ribonuclease P protein component; subspecies Mycoplasma mycoides mycoides SC
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Aquifex aeolicus
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Manually annotated by BRENDA team
no activity in Nanoarchaeum equitans
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
component RNPA
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Pyrobaculum sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain SCWY10
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
synthetic construct
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
ribonuclease P protein component
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
10Sa RNA + H2O
?
show the reaction diagram
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?
4.55 rRNA precursor + H2O
mature 4.55 rRNA + 5'-oligonucleotide
show the reaction diagram
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-
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?
4.5S RNA precursor + H2O
mature 4.5S RNA + 5'-oligonucleotide
show the reaction diagram
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RNA processing
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?
4.5S RNA, precursor to + H2O
?
show the reaction diagram
C4 antisense RNA + H2O
?
show the reaction diagram
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from bacteriophages P1 and P7
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?
chloroplastic pre-tRNAPhe + H2O
chloroplastic tRNAPhe + 5' leader of tRNA
show the reaction diagram
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-
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?
deproteinized pre-rRNA + H2O
mature deproteinized rRNA + 5'-oligonucleotide
show the reaction diagram
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large number of discrete cleavage sites
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?
hepatitis C virus RNA + H2O
?
show the reaction diagram
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the catalytic RNase P RNA cleaves near the AUG start codon
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?
influenza virus mRNA + H2O
?
show the reaction diagram
mitochondrial pre-tRNACys + H2O
mitochondrial tRNACys + 5' leader of tRNA
show the reaction diagram
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-
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?
pbuE adenine riboswitch + H2O
?
show the reaction diagram
phage antisense RNA (C4) + H2O
?
show the reaction diagram
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-
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?
phage f2RNA + H2O
?
show the reaction diagram
phi80-induced RNA + H2O
?
show the reaction diagram
polycistronic his operon mRNA precursor + H2O
?
show the reaction diagram
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?
polycistronic mRNA precursor + H2O
mature mRNAs + ?
show the reaction diagram
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RNA processing
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?
pre-rRNA + H2O
?
show the reaction diagram
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RNase MRP is involved with the maturation of pre-rRNA but cleaves RNA primers in mitochondria and localizes to cytoplasmic P-bodies where it takes part in cell cycle-regulated turnover of selected mRNAs
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?
pre-rRNA + H2O
mature rRNA
show the reaction diagram
pre-rRNA + H2O
mature rRNA + 5'-oligonucleotide
show the reaction diagram
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RNase MRP
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?
pre-tRNA + H2O
?
show the reaction diagram
pre-tRNA + H2O
mat-tRNA + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNA + H2O
mature tRNA + 5'-terminal oligonucleotide
show the reaction diagram
pre-tRNA + H2O
tRNA
show the reaction diagram
pre-tRNA + H2O
tRNA + 5' leader of tRNA
show the reaction diagram
pre-tRNA + H2O
tRNA + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA + H2O
tRNA + RNA sequence
show the reaction diagram
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a pre-tRNA is trapped on the CCA site and 5'-leader site first to form the Michaelis-complex. On this step, the shape of a substrate RNA is not recognized by the enzyme. After that, the T-arm site and the bottom half site cooperatively examine the shape of the substrate to achieve the transition state conformation. After the cleavage of the 5'-leader sequence, the enzyme-product complex turns to the non-transition state conformation, and the cleaved product is released from the enzyme
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?
pre-tRNA mimic + H2O
?
show the reaction diagram
pre-tRNA mimic a + H2O
?
show the reaction diagram
pre-tRNA mimic b + H2O
?
show the reaction diagram
pre-tRNA mimic c + H2O
?
show the reaction diagram
pre-tRNA mimic d + H2O
?
show the reaction diagram
pre-tRNA precursor + H2O
mat-tRNA + RNA
show the reaction diagram
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?
pre-tRNA precursor + H2O
tRNA + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA-Gly + H2O
tRNA-Gly + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA-Met + H2O
tRNA-Met + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNA-Tyr + H2O
tRNA-Tyr + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNA-Val + H2O
tRNA-Val + 5'-oligoribonucleotide
show the reaction diagram
-
-
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?
pre-tRNA3Pro + H2O
mature tRNA3Pro + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNAAla + H2O
mature tRNAAla + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of fly substrate, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNAAsp + H2O
?
show the reaction diagram
pre-tRNAAsp + H2O
tRNAAsp + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNAAsp + H2O
tRNAsp + 5'-oligoribonucleotide
show the reaction diagram
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pre-tRNA binding affinities for RNase P are enhanced by sequence-specific contacts between the fourth pre-tRNA nucleotide on the 5' side of the cleavage site (N(-4)) and the RNase P protein (P protein) subunit. RNase P has a higher affinity for pre-tRNA with adenosine at N(-4), and this binding preference is amplified at physiological divalent ion concentrations. Binds A(-4) pre-tRNA 20fold more tightly than the G(-4) substrate, and binds the C(-4) and U(-4) substrates with intermediate affinity. F20 and Y34 contribute to selectivity at N(-4). The hydroxyl group of Y34 enhances selectivity, likely by forming a hydrogen bond with the N(-4) nucleotide
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?
pre-tRNAHis + H2O
mature tRNAHis + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of fly substrate, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNALeu + H2O
tRNALeu + 5' leader of tRNA
show the reaction diagram
pre-tRNAMet_ini + H2O
mature tRNAMet_ini + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of fly initiator methionine tRNA, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNAPhe + H2O
?
show the reaction diagram
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?
pre-tRNAPhe + H2O
tRNAPhe + 5'-oligoribonucleotide
show the reaction diagram
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?
pre-tRNATyr + H2O
mature tRNATyr + 5'-oligonucleotide
show the reaction diagram
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cleavage of 5'-terminal oligonucleotide of human substrate, hyperprocessing
generates 5'-phosphate,3'-hydroxyl-product
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?
pre-tRNATyr + H2O
mature tRNATyr + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNATyr + H2O
tRNATyr + 5' leader of tRNA
show the reaction diagram
pre-tRNATyr + H2O
tRNATyr + 5'-oligoribonucleotide
show the reaction diagram
pre-tRNATyr precursor + H2O
mature tRNATyr + 5'-terminal oligonucleotide
show the reaction diagram
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?
precursor tRNA + H2O
77- and 35-RNA fragments
show the reaction diagram
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ribonuclease P is the endonuclease that removes the leader fragments from the 5'-ends of precursor tRNAs
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?
precursor tRNA + H2O
?
show the reaction diagram
precursor tRNA Gly + H2O
mature tRNA Gly + 5'-GGAUUUUCCCUUUC
show the reaction diagram
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5' flank with homogeneous 3' end, CCAGUC-3'
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?
precursors to 4.5 S RNA + H2O
?
show the reaction diagram
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?
ptRNATyr + H2O
?
show the reaction diagram
ssRNA oligonucleotide + H2O
5'-phospho-3'-hydroxy-ribonucleotides
show the reaction diagram
SupS1 precursor + H2O
?
show the reaction diagram
-
-
-
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?
syntaxin18 mRNA + H2O
?
show the reaction diagram
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?
tmRNA + H2O
?
show the reaction diagram
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?
tmRNA precursor + H2O
mature tmRNA + 5'-oligonucleotide
show the reaction diagram
-
RNA processing
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?
tRNA precursor + H2O
mature tRNA + 5'-oligonucleotide
show the reaction diagram
tRNA precursor + H2O
mature tRNA + 5'-terminal oligonucleotide
show the reaction diagram
tRNA-like pseudoknotted structures in viral RNA + H2O
processed RNA + ?
show the reaction diagram
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tRNA-like pseudoknotted structures in viral RNA
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?
tRNAAsp precursor + H2O
mature tRNAAsp + 5'-terminal oligonucleotide
show the reaction diagram
tRNALeu5 + H2O
?
show the reaction diagram
tRNAPhe (A+1) precursor + H2O
mature tRNAPhe + 5'-terminal oligonucleotide
show the reaction diagram
tRNAPhe (G+1) precursor + H2O
mature tRNAPhe + 5'-terminal oligonucleotide
show the reaction diagram
tRNAPhe precursor + H2O
mature tRNAPhe + 5'-terminal oligonucleotide
show the reaction diagram
-
maize tRNA substrate, structure
cleavage site determination
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?
tRNATyr precursor + H2O
mature tRNATyr + 5'-oligonucleotide
show the reaction diagram
tRNATyr precursor + H2O
mature tRNATyr + 5'-terminal oligonucleotide
show the reaction diagram
-
-
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?
tRNATyrUAG precursor + H2O
?
show the reaction diagram
-
RNase P cleavage of this substrate generates a 5' matured tRNA with a 7 base pair amino acceptor stem
-
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?
additional information
?
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