Information on EC 3.1.26.10 - ribonuclease IX

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.1.26.10
-
RECOMMENDED NAME
GeneOntology No.
ribonuclease IX
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of poly(U) or poly(C) to fragments terminated by 3'-hydroxy and 5'-phosphate groups
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-99-4
not distinguished from EC 3.1.27.6
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
homometabolous insect
-
-
Manually annotated by BRENDA team
human
-
-
Manually annotated by BRENDA team
yeast, strain W303alpha
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
mRNA + H2O
?
show the reaction diagram
poly(C) x (dG)12-18 polynucleotide template primer + H2O
?
show the reaction diagram
RNA + H2O
?
show the reaction diagram
-
RNA isolated from six-day-old larvae or rat liver
-
-
?
rRNA + H2O
?
show the reaction diagram
-
26S, 18S and 5S rRNA, processing of rRNA, naked rRNA molecules are sensitive to the purified enzyme, enzyme has no activity to the ribosome integrated rRNA
-
-
?
synthetic homopolynucleotide + H2O
?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
mRNA + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
strong stimulation of enzyme activity
Ca2+
-
strong stimulation of enzyme activity
Cs+
-
rather weak stimulation
Mg2+
-
optimum enzyme activity at 0.3-0.5 mM
Mn2+
-
strong stimulation of enzyme activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Al3+
-
strong inhibition
HP-RNase antibody
-
-
-
Mg2+
-
1 mM reduces activity by 15%
placental RNase inhibitor
-
-
-
Zn2+
-
strong inhibition
additional information
-
not inhibited by the placental RNase inhibitor
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
-
Tris-HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immortalized umbilical vein endothelial cells EaHy 926
Manually annotated by BRENDA team
-
HuLEC cell
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000 - 26000
-
molecular heterogeneity due to different degrees of N-glycosylation, SDS-PAGE
25000
-
gel filtration
29000
-
SDS-PAGE
34000
-
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 65
-
thermolabile, practically loses most of its activity after 10 min at 60C, at 65C enzyme is almost inactivated within the first min, at 99C it is completely inactive right from the beginning
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very unstable, DTT, GSH or 2-mercaptoethanol stabilizes
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80C, storage does not modify the specific activity of the purified protein
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE