Information on EC 3.1.22.5 - deoxyribonuclease X

for references in articles please use BRENDA:EC3.1.22.5
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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
3.1.22.5
-
RECOMMENDED NAME
GeneOntology No.
deoxyribonuclease X
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of supercoiled plasma DNA to linear DNA duplexes
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endonucleolytic cleavage of supercoiled plasma DNA to linear DNA duplexes
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
97002-82-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
Escherichia coli B / ATCC 11303
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
linear double-stranded DNA + H2O
?
show the reaction diagram
supercoiled plasma DNA + H2O
linear DNA duplexes
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
supercoiled plasma DNA + H2O
linear DNA duplexes
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
absolute requirement for a divalent cation, optimum concentration: 1.5 mM, inhibition at higher concentrations
Mg2+
-
absolute requirement for a divalent cation, optimum concentration: 5 mM, inhibition at higher concentrations
additional information
-
very little activity with Mn2+ or Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
at concentrations above 1.5 mM
Mg2+
-
at concentrations above 5 mM
single-stranded DNA
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-
Tris-HCl
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optimum concentration at 10-150 mM, inhibition near 150 mM
additional information
-
no effect: adenosine, thymidine, RNA and double-stranded DNA
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 8.8
-
pBR322
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
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x * 49000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE