Information on EC 3.1.22.2 - Aspergillus deoxyribonuclease K1

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The expected taxonomic range for this enzyme is: Aspergillus oryzae

EC NUMBER
COMMENTARY hide
3.1.22.2
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RECOMMENDED NAME
GeneOntology No.
Aspergillus deoxyribonuclease K1
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
3'-phosphopolynucleotide + 3'-phosphooligonucleotide
show the reaction diagram
DNA + H2O
?
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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30% activation at 0.02 M and 10% activation at 0.001 M
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
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90% inhibition at 0.001 M
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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49.7 U, reducing absorbance at 260 nm/h/mg DNA digested
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.5 - 9.5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inactivation up to 45øC
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-5°C in polyethylen glycol or glycerol for 3 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2fold fractionation on DEAE-cellulose with NaCL gradient elution, gel filtration on sephadex G-50
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