Information on EC 3.1.21.9 - T4 deoxyribonuclease IV

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The expected taxonomic range for this enzyme is: Enterobacteria phage T4

EC NUMBER
COMMENTARY hide
3.1.21.9
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RECOMMENDED NAME
GeneOntology No.
T4 deoxyribonuclease IV
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage of the 5' phosphodiester bond of deoxycytidine in single-stranded DNA
show the reaction diagram
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-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-(dA)10(dC)5(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)12(dC) (dA)12-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)5(dC)15(dA)5-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)7(dC)11(dA)7-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)7dAdAdCdAdA(dA)7-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)7dAdTdCdAdA(dA)7-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)8dGdTdCdAdG(dA)8-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9(dC)6(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9(dC)7(dA)9-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdAdAdAdAdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdAdCdAdAdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdCdAdTdTdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdCdTdAdTdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdCdTdTdAdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdCdTdTdTdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdGdCdGdGdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdGdGdGdGdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdTdCdTdTdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdTdTdCdTdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdTdTdTdCdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdTdTdTdTdC(dA)10-3' + H2O
?
show the reaction diagram
-
-
-
-
?
5'-(dA)9dCdTdTdTdTdTdC(dA)9-3' + H2O
?
show the reaction diagram
-
-
-
-
?
bacteriophage fd DNA + H2O
?
show the reaction diagram
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digestion results in about 25 fragments that migrate as discrete bands on polyacrylamide gel. These bands give unique fingerprints, indicating that the enzyme has considerable specificity
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?
DNA + H2O
?
show the reaction diagram
DNA where cytosine is partially or completely replaced by hydroxymethylcytosine + H2O
?
show the reaction diagram
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DNA where cytosine is partially or completely replaced by hydroxymethylcytosine is degraded in vivo by T4 endonuclease II, EC 3.1.21.8, and endonuclease IV, EC 3.1.21.9
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-
?
phage fd DNA + H2O
?
show the reaction diagram
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-
-
-
?
polydeoxycytydilate + H2O
?
show the reaction diagram
-
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product is acid-soluble material ending excluively in 5'-dCMP
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
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the phage T4 enzyme is involved in degradation of host DNA
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-
?
DNA where cytosine is partially or completely replaced by hydroxymethylcytosine + H2O
?
show the reaction diagram
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DNA where cytosine is partially or completely replaced by hydroxymethylcytosine is degraded in vivo by T4 endonuclease II, EC 3.1.21.8, and endonuclease IV, EC 3.1.21.9
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?
additional information
?
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enzyme is capable of cleaving at the atypical apurinic-apyrimidinic site created by the action of T4 PD-DNA glycosylase. Exonuclease III and endonuclease IV are equally efficient in repair
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
may substitute for Mg2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
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0.05 M, 70% inhibition
K3PO4
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0.05 M, 80% inhibition
NH4Cl
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0.05 M, 47% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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activity of endonuclease IV is stimulated in the presence of a wild-type dCMP hydroxymethylase
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0126
5'-(dA)10(dC)5(dA)10-3'
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pH 8.0, 37C
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0.0141
5'-(dA)12(dC) (dA)12-3'
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pH 8.0, 37C
-
0.006
5'-(dA)5(dC)15(dA)5-3'
-
pH 8.0, 37C
-
0.0051
5'-(dA)7(dC)11(dA)7-3'
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pH 8.0, 37C
-
0.023
5'-(dA)7dAdAdCdAdA(dA)7-3'
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pH 8.0, 37C
-
0.018
5'-(dA)7dAdTdCdAdA(dA)7-3'
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pH 8.0, 37C
-
0.017
5'-(dA)8dGdTdCdAdG(dA)8-3'
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pH 8.0, 37C
-
0.0058
5'-(dA)9(dC)6(dA)10-3'
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pH 8.0, 37C
-
0.0063
5'-(dA)9(dC)7(dA)9-3'
-
pH 8.0, 37C
-
0.0088
5'-(dA)9dCdAdAdAdAdC(dA)10-3'
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pH 8.0, 37C
-
0.0054
5'-(dA)9dCdAdCdAdAdC(dA)10-3'
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pH 8.0, 37C
-
0.0052
5'-(dA)9dCdCdAdTdTdC(dA)10-3'
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pH 8.0, 37C
-
0.0049
5'-(dA)9dCdCdTdAdTdC(dA)10-3'
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pH 8.0, 37C
-
0.0054
5'-(dA)9dCdCdTdTdAdC(dA)10-3', 5'-(dA)9dCdCdTdTdTdC(dA)10-3'
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pH 8.0, 37C
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0.0068
5'-(dA)9dCdGdCdGdGdC(dA)10-3'
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pH 8.0, 37C
-
0.0093
5'-(dA)9dCdGdGdGdGdC(dA)10-3'
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pH 8.0, 37C
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0.006
5'-(dA)9dCdTdCdTdTdC(dA)10-3'
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pH 8.0, 37C
-
0.005
5'-(dA)9dCdTdTdCdTdC(dA)10-3'
-
pH 8.0, 37C
-
0.0058
5'-(dA)9dCdTdTdTdCdC(dA)10-3'
-
pH 8.0, 37C
-
0.0088
5'-(dA)9dCdTdTdTdTdC(dA)10-3'
-
pH 8.0, 37C
-
0.0126
5'-(dA)9dCdTdTdTdTdTdC(dA)9-3'
-
pH 8.0, 37C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00576
5'-(dA)7dAdAdCdAdA(dA)7-3'
Enterobacteria phage T4
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pH 8.0, 37C
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0.0167
5'-(dA)7dAdTdCdAdA(dA)7-3'
Enterobacteria phage T4
-
pH 8.0, 37C
-
0.0087
5'-(dA)8dGdTdCdAdG(dA)8-3'
Enterobacteria phage T4
-
pH 8.0, 37C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
5'-(dA)7dAdAdCdAdA(dA)7-3'
Enterobacteria phage T4
-
pH 8.0, 37C
194840
0.08
5'-(dA)7dAdTdCdAdA(dA)7-3'
Enterobacteria phage T4
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pH 8.0, 37C
194841
0.05
5'-(dA)8dGdTdCdAdG(dA)8-3'
Enterobacteria phage T4
-
pH 8.0, 37C
194842
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
840000
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pH 7.5, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.4 - 9.2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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62% of maximum activity
8.5
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62% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified about 150fold from T4 phage-infected Escherichia coli B
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recombinant protein
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in wheat germ-free expression system
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