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Information on EC 3.1.21.4 - type II site-specific deoxyribonuclease and Organism(s) Serratia marcescens and UniProt Accession P14229

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This record set is specific for:
Serratia marcescens
UNIPROT: P14229 not found.
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The taxonomic range for the selected organisms is: Serratia marcescens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
hide
endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Synonyms
restriction enzyme, restriction endonuclease, ecori, hindiii, bamhi, pvuii, haeiii, hpaii, bglii, ecorv, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
type II restriction enzyme
-
DNA restriction endonuclease
-
-
-
-
DNA restriction enzyme
-
-
-
-
EC 3.1.23
-
-
-
-
EC 3.1.24
-
-
-
-
Endonuclease AbrI
-
-
-
-
Endonuclease AccI
-
-
-
-
Endonuclease AgeI
-
-
-
-
Endonuclease ApaLI
-
-
-
-
Endonuclease AvaI
-
-
-
-
Endonuclease BamHI
-
-
-
-
Endonuclease BanI
-
-
-
-
Endonuclease BglI
-
-
-
-
Endonuclease BglII
-
-
-
-
Endonuclease BsoBI
-
-
-
-
Endonuclease Bsp6I
-
-
-
-
Endonuclease BstVI
-
-
-
-
Endonuclease BsuBI
-
-
-
-
Endonuclease BsuFI
-
-
-
-
Endonuclease BsuRI
-
-
-
-
Endonuclease CeqI
-
-
-
-
Endonuclease Cfr10I
-
-
-
-
Endonuclease Cfr9I
-
-
-
-
Endonuclease CfrBI
-
-
-
-
Endonuclease CviAII
-
-
-
-
Endonuclease CviJI
-
-
-
-
Endonuclease DdeI
-
-
-
-
Endonuclease DpnI
-
-
-
-
Endonuclease DpnII
-
-
-
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Endonuclease Eco47I
-
-
-
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Endonuclease Eco47II
-
-
-
-
Endonuclease EcoRI
-
-
-
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Endonuclease EcoRII
-
-
-
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Endonuclease EcoRV
-
-
-
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Endonuclease FokI
-
-
-
-
Endonuclease HaeII
-
-
-
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Endonuclease HaeIII
-
-
-
-
Endonuclease HgAI
-
-
-
-
Endonuclease HgiBI
-
-
-
-
Endonuclease HgiCI
-
-
-
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Endonuclease HgiCII
-
-
-
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Endonuclease HgiDI
-
-
-
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Endonuclease HgiEI
-
-
-
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Endonuclease HgiGI
-
-
-
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Endonuclease HhaII
-
-
-
-
Endonuclease HincII
-
-
-
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Endonuclease HindII
-
-
-
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Endonuclease HindIII
-
-
-
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Endonuclease HindVP
-
-
-
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Endonuclease HinfI
-
-
-
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Endonuclease HpaI
-
-
-
-
Endonuclease HpaII
-
-
-
-
Endonuclease HphI
-
-
-
-
Endonuclease KpnI
-
-
-
-
Endonuclease LlaDCHI
-
-
-
-
Endonuclease MamI
-
-
-
-
Endonuclease MboI
-
-
-
-
Endonuclease MboII
-
-
-
-
Endonuclease MjaI
-
-
-
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Endonuclease MjaII
-
-
-
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Endonuclease MjaIII
-
-
-
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Endonuclease MjaIV
-
-
-
-
Endonuclease MjaV
-
-
-
-
Endonuclease MjaVIP
-
-
-
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Endonuclease MspI
-
-
-
-
Endonuclease MthTI
-
-
-
-
Endonuclease MthZI
-
-
-
-
Endonuclease MunI
-
-
-
-
Endonuclease MwoI
-
-
-
-
Endonuclease NaeI
-
-
-
-
Endonuclease NgoBI
-
-
-
-
Endonuclease NgoBV
-
-
-
-
Endonuclease NgoFVII
-
-
-
-
Endonuclease NgoMIV
-
-
-
-
Endonuclease NgoPII
-
-
-
-
Endonuclease NlaIII
-
-
-
-
Endonuclease NlaIV
-
-
-
-
Endonuclease NmeDIP
-
-
-
-
Endonuclease NspV
-
-
-
-
Endonuclease PaeR7I
-
-
-
-
Endonuclease PstI
-
-
-
-
Endonuclease PvuI
-
-
-
-
Endonuclease PvuII
-
-
-
-
Endonuclease RsrI
-
-
-
-
Endonuclease SacI
-
-
-
-
Endonuclease SalI
-
-
-
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Endonuclease Sau3AI
-
-
-
-
Endonuclease Sau96I
-
-
-
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Endonuclease ScaI
-
-
-
-
Endonuclease ScrFI
-
-
-
-
Endonuclease SfiI
-
-
-
-
Endonuclease SinI
-
-
-
-
Endonuclease SmaI
-
-
-
-
Endonuclease SsoII
-
-
-
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Endonuclease StsI
-
-
-
-
Endonuclease TaqI
-
-
-
-
Endonuclease TthHB8I
-
-
-
-
Endonuclease XamI
-
-
-
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Endonuclease XcyI
-
-
-
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LlaII
-
-
-
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nuclease, deoxyribonucleic restriction endo-
-
-
-
-
nuclease, restriction endodeoxyribo-
-
-
-
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R.AbrI
-
-
-
-
R.AccI
-
-
-
-
R.AgeI
-
-
-
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R.ApaLI
-
-
-
-
R.AvaI
-
-
-
-
R.BamHI
-
-
-
-
R.BanI
-
-
-
-
R.BglI
-
-
-
-
R.BglII
-
-
-
-
R.BsoBI
-
-
-
-
R.Bsp6I
-
-
-
-
R.BstVI
-
-
-
-
R.BsuBI
-
-
-
-
R.BsuRI
-
-
-
-
R.CeqI
-
-
-
-
R.Cfr10I
-
-
-
-
R.Cfr9I
-
-
-
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R.CfrBI
-
-
-
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R.CviAII
-
-
-
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R.CviJI
-
-
-
-
R.DdeI
-
-
-
-
R.DpnI
-
-
-
-
R.DpnII
-
-
-
-
R.Eco47I
-
-
-
-
R.Eco47II
-
-
-
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R.EcoRI
-
-
-
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R.EcoRII
-
-
-
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R.EcoRV
-
-
-
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R.FokI
-
-
-
-
R.HaeII
-
-
-
-
R.HaeIII
-
-
-
-
R.HgAI
-
-
-
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R.HgiBI
-
-
-
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R.HgiCI
-
-
-
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R.HgiCII
-
-
-
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R.HgiDI
-
-
-
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R.HgiEI
-
-
-
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R.HgiGI
-
-
-
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R.HhaII
-
-
-
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R.HincII
-
-
-
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R.HindII
-
-
-
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R.HindIII
-
-
-
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R.HindVP
-
-
-
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R.HinfI
-
-
-
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R.HpaI
-
-
-
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R.HpaII
-
-
-
-
R.HphI
-
-
-
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R.KpnI
-
-
-
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R.LlaDCHI
-
-
-
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R.MamI
-
-
-
-
R.MboI
-
-
-
-
R.MboII
-
-
-
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R.MjaI
-
-
-
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R.MjaII
-
-
-
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R.MjaIII
-
-
-
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R.MjaIV
-
-
-
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R.MjaV
-
-
-
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R.MjaVIP
-
-
-
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R.MspI
-
-
-
-
R.MthTI
-
-
-
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R.MthZI
-
-
-
-
R.MunI
-
-
-
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R.MwoI
-
-
-
-
R.NaeI
-
-
-
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R.NgoBI
-
-
-
-
R.NgoBV
-
-
-
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R.NgoFVII
-
-
-
-
R.NgoI
-
-
-
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R.NgoMIV
-
-
-
-
R.NgoPII
-
-
-
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R.NgoV
-
-
-
-
R.NgoVII
-
-
-
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R.NlaIII
-
-
-
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R.NlaIV
-
-
-
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R.NmeDIP
-
-
-
-
R.NspV
-
-
-
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R.PaeR7I
-
-
-
-
R.PstI
-
-
-
-
R.PvuI
-
-
-
-
R.PvuII
-
-
-
-
R.RsrI
-
-
-
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R.SacI
-
-
-
-
R.SalI
-
-
-
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R.Sau3AI
-
-
-
-
R.Sau96I
-
-
-
-
R.ScaI
-
-
-
-
R.ScrFI
-
-
-
-
R.SfiI
-
-
-
-
R.SinI
-
-
-
-
R.SmaI
-
-
-
-
R.SsoII
-
-
-
-
R.StsI
-
-
-
-
R.TaqI
-
-
-
-
R.TthHB8I
-
-
-
-
R.XamI
-
-
-
-
R.XcyI
-
-
-
-
restriction endodeoxyribonuclease
-
-
-
-
restriction endonuclease
-
-
-
-
restriction enzyme
-
-
-
-
type II restriction enzyme
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9075-08-5
not distinguished from EC 3.1.21.5
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
show the reaction diagram
-
-
-
?
DNA + H2O
double-stranded DNA fragments with terminal 5'-phosphates
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required for activity
Mg2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
increasing proportions of uracil in DNA substrates increases inhibition of restriction enzyme digests
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 37
-
SmaI
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
T2SM_SERMA
247
0
28782
Swiss-Prot
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Roberts, R.J.
Restriction enzymes and their isoschizomers
Nucleic Acids Res.
18
2331-2365
1990
Acetobacter aceti, Gluconobacter oxydans, Acetobacter pasteurianus, Acinetobacter lwoffii, Streptomyces phaeochromogenes, Thalassobius gelatinovorus, Anabaena cylindrica, Dolichospermum flos-aquae, Anabaena subcylindrica, Trichormus variabilis, Synechocystis sp., Aphanothece halophytica, Cellulosimicrobium cellulans, Geobacillus stearothermophilus, Bacillus amyloliquefaciens, Brevibacillus brevis, [Bacillus] caldolyticus, Bacillus cereus, Weizmannia coagulans, Bacillus subtilis, Bacillus pumilus, Lysinibacillus sphaericus, uncultured bacterium, Bifidobacterium longum subsp. infantis, Curtobacterium albidum, Caryophanon latum, Chloroflexus aurantiacus, Chromobacterium violaceum, Citrobacter freundii, Dactylococcus salina, Deinococcus radiodurans, Deinococcus radiophilus, Desulfovibrio desulfuricans, Streptococcus pneumoniae, Escherichia coli, Enterococcus faecalis, Eucapsis sp., Flavobacterium aquatile, Chryseobacterium indologenes, Planomicrobium okeanokoites, Thermus thermophilus, Frankia sp., Fusobacterium nucleatum, Haemophilus aegyptius, Avibacterium paragallinarum, Haemophilus haemolyticus, Haemophilus influenzae, Haemophilus parahaemolyticus, Haemophilus parainfluenzae, Herpetosiphon aurantiacus, Klebsiella pneumoniae, Methanothermobacter wolfeii, Methanococcus aeolicus, Methylophilus methylotrophus, Psychrobacter urativorans, Micrococcus luteus, Micrococcus lylae, Staphylococcus aureus, Microcoleus sp., Moraxella bovis, Moraxella nonliquefaciens, Mycoplasmopsis fermentans, Bergeriella denitrificans, Neisseria lactamica, Neisseria mucosa heidelbergensis, Lentzea aerocolonigenes, Nocardia argentinensis, Gordonia rubripertincta, Nocardia otitidiscaviarum, Rhodococcus ruber, Nostoc sp., Plesiomonas shigelloides, Proteus vulgaris, Pseudomonas sp., Pseudomonas putida, Pseudomonas fluorescens, Paucimonas lemoignei, Stenotrophomonas maltophilia, Pseudomonas stutzeri, Rhizobium leguminosarum, Cereibacter sphaeroides, Salmonella enterica subsp. enterica serovar Typhi, Serratia marcescens, Sphaerotilus natans, Sphaerotilus sp., Arthrospira platensis, Streptomyces achromogenes, Lactococcus cremoris, Enterococcus durans, Streptomyces albus, Streptomyces caespitosus, Streptomyces fimbriatus, Thermus aquaticus, Thermus filiformis, Thermus sp., Vibrio sp., Xanthomonas campestris pv. badrii, Xanthomonas campestris, Xanthomonas phaseoli pv. manihotis, Xanthomonas vasicola, Xanthomonas citri pv. malvacearum, Acetobacter pasteurianus ApaLI
Manually annotated by BRENDA team
Wells, R.D.; Klein, R.D.; Singleton, C.K.
Type II restriction enzymes
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
14
157-191
1981
Trichormus variabilis, Cellulosimicrobium cellulans, Geobacillus stearothermophilus, Bacillus amyloliquefaciens, [Bacillus] caldolyticus, Bacillus subtilis, Lysinibacillus sphaericus, Desulfovibrio desulfuricans, Streptococcus pneumoniae, Escherichia coli, Enterococcus faecalis, Thermus thermophilus, Fusobacterium nucleatum, Haemophilus aegyptius, Avibacterium paragallinarum, Haemophilus haemolyticus, Haemophilus influenzae, Haemophilus parahaemolyticus, Haemophilus parainfluenzae, Herpetosiphon aurantiacus, Staphylococcus aureus, Moraxella bovis, Providencia stuartii, Cereibacter sphaeroides, Serratia marcescens, Streptomyces achromogenes, Streptomyces albus, Xanthomonas campestris pv. badrii, Xanthomonas vasicola, Xanthomonas citri pv. malvacearum, Staphylococcus aureus Sau96I
-
Manually annotated by BRENDA team
Glenn, T.C.; Waller, D.R.; Braun, M.J.
Increasing proportions of uracil in DNA substrates increases inhibition of restriction enzyme digests
Biotechniques
17
1086-1090
1994
Acetobacter pasteurianus, Geobacillus stearothermophilus, Bacillus amyloliquefaciens, Caryophanon latum, Escherichia coli, Haemophilus influenzae, Klebsiella pneumoniae, Nocardia otitidiscaviarum, Pseudomonas sp., Serratia marcescens, Streptomyces achromogenes, Xanthomonas campestris pv. badrii, Acetobacter pasteurianus ApaI, Geobacillus stearothermophilus BstXI, Geobacillus stearothermophilus BstZI
Manually annotated by BRENDA team
Orlowski, J.; Bujnicki, J.M.
Structural and evolutionary classification of type II restriction enzymes based on theoretical and experimental analyses
Nucleic Acids Res.
36
3552-3569
2008
Trichormus variabilis, Caryophanon latum, Helicobacter pylori, Helicobacter pylori (Q9ZLF0), Helicobacter pylori (Q9ZN14), Methylococcus capsulatus, Pyrococcus horikoshii, Staphylococcus hominis, Sulfolobus acidocaldarius, Thermus aquaticus, Corynebacterium striatum, Streptomyces achromogenes (O31074), Haemophilus parahaemolyticus (P00643), Serratia marcescens (P14229), Haemophilus influenzae (P20588), Staphylococcus aureus (P23736), Herpetosiphon aurantiacus (P25260), Nostoc sp. (P35677), Citrobacter freundii (Q04852), Sporosarcina sp. (Q194N8), Micrococcus sp. (Q2I0D9), Methanocaldococcus jannaschii (Q58723), Methanocaldococcus jannaschii (Q58895), Moraxella sp. (Q6S4U8), Sphaerotilus natans (Q6SA25), Cereibacter sphaeroides (Q6SA27), Geobacillus stearothermophilus (Q8L3A5), Lactococcus lactis (Q93K18), Thermus filiformis (Q9F8S3), Thalassobius gelatinovorus (Q9KHV6), Xanthomonas vasicola (Q9KVZ7), Neisseria meningitidis (Q9RLM3), Corynebacterium striatum M82B, Moraxella sp. A1 (Q6S4U8), Micrococcus sp. NEB 446 (Q2I0D9)
Manually annotated by BRENDA team