Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.1.21.2 - deoxyribonuclease IV and Organism(s) Thermotoga maritima and UniProt Accession Q9WYJ7

for references in articles please use BRENDA:EC3.1.21.2
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Thermotoga maritima
UNIPROT: Q9WYJ7 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Thermotoga maritima
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products
Synonyms
endonuclease iv, apurinic/apyrimidinic endonuclease, endodeoxyribonuclease, endo iv, endoii, e. coli endonuclease iv, endoiv, mtbnfo, t4 endonuclease iv, tthnfo, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
deoxriboendonuclease
-
-
-
-
DNA-adenine-transferase
-
-
-
-
E. coli endonuclease IV
-
-
-
-
endodeoxyribonuclease IV
-
-
-
-
nuclease, endodeoxyribo-oder redoxyendonuclease
-
-
-
-
T4 endonuclease IV
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
37211-67-9
-
58591-37-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
plasmid containing AP sites + H2O
?
show the reaction diagram
the enzyme has apurinic/apyrimidinic endonuclease activity, 3'-phosphatase activity, and 3'-repair diesterase activity for phosphoglycolates and trans-4-hydroxy-2-pentenal-5-phosphates
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
contains Mg2+
Mn2+
contains Mn2+, the enzyme with Mn2+ and Zn2+ is more active than with only Zn2+
Zn2+
contains Zn2+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the presence of this enzyme, which is part of the DNA base excision repair pathway, suggests that thermophiles use a mechanism similar to that used by mesophiles to deal with the large number of abasic sites that arise in their chromosomes due to the increased rates of DNA damage at elevated temperatures
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32400
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 32400, calculated from sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 100 mM Tris-HCl, pH 9.0, 4 mM dithiothreitol, 1.5% saturated MgSO4, 16% (w/v) ethylene glycol, 20% (w/v) polyethylene glycol monomethyl ether 2000
using the sitting-drop vapor-diffusion method
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
88
denatures above
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the gene is expressed under the direction of an IPTG-inducible T7 promoter, and the Thermotoga maritima endonuclease IV is purified as a His-tagged protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hughes, R.C.; Tomanicek, S.J.; Ng, J.D.; Coates, L.
Purification, crystallization and preliminary crystallographic analysis of a thermostable endonuclease IV from Thermotoga maritima
Acta Crystallogr. Sect. F
65
1317-1319
2009
Thermotoga maritima (Q9WYJ7), Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 (Q9WYJ7)
Manually annotated by BRENDA team
Tsutakawa, S.E.; Shin, D.S.; Mol, C.D.; Izumi, T.; Arvai, A.S.; Mantha, A.K.; Szczesny, B.; Ivanov, I.N.; Hosfield, D.J.; Maiti, B.; Pique, M.E.; Frankel, K.A.; Hitomi, K.; Cunningham, R.P.; Mitra, S.; Tainer, J.A.
Conserved structural chemistry for incision activity in structurally non-homologous apurinic/apyrimidinic endonuclease APE1 and endonuclease IV DNA repair enzymes
J. Biol. Chem.
288
8445-8455
2013
Thermotoga maritima (Q9WYJ7), Thermotoga maritima DSM 3109 (Q9WYJ7)
Manually annotated by BRENDA team
Haas, B.; Sandigursky, M.; Tainer, J.; Franklin, W.; Cunningham, R.
Purification and characterization of Thermotoga maritima endonuclease IV, a thermostable apurinic/apyrimidinic endonuclease and 3-repair diesterase
J. Bacteriol.
181
2834-2839
1999
Thermotoga maritima (Q9WYJ7), Thermotoga maritima, Thermotoga maritima DSM 3109 (Q9WYJ7)
Manually annotated by BRENDA team