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Information on EC 3.1.21.1 - deoxyribonuclease I and Organism(s) Gallus gallus and UniProt Accession Q9YGI5

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This record set is specific for:
Gallus gallus
UNIPROT: Q9YGI5 not found.
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The taxonomic range for the selected organisms is: Gallus gallus
The enzyme appears in selected viruses and cellular organisms
Synonyms
lactoferrin, dna polymerase, dnase i, colicin, diphtheria toxin, dnaase, deoxyribonuclease, dnasei, deoxyribonuclease i, crm197, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
deoxyribonuclease
-
-
-
-
deoxyribonuclease A
-
-
-
-
deoxyribonucleic phosphatase
-
-
-
-
desoxyribonuclease
-
-
-
-
DNA endonuclease
-
-
-
-
DNase
-
-
-
-
DNase I
-
-
-
-
Dornase alfa
-
-
-
-
endodeoxyribonuclease I
-
-
-
-
Escherichia coli endonuclease I
-
-
-
-
nuclease, deoxyribo-
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-
-
-
nuclease, Escherichia coli endo-, I
-
-
-
-
pancreatic deoxyribonuclease
-
-
-
-
pancreatic DNase
-
-
-
-
streptodornase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
show the reaction diagram
preference for double-stranded DNA
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9003-98-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
double-stranded DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
DNase I hypersensitive sites of chromatin near the 5'-ends of some genes, necessary for transcription by RNA polymerase II
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
double-stranded DNA + H2O
5'-phosphooligonucleotides + ?
show the reaction diagram
-
DNase I hypersensitive sites of chromatin near the 5'-ends of some genes, necessary for transcription by RNA polymerase II
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
half-maximal activation at 4 mM
Mn2+
half-maximal activation at 1 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
slight inhibition
iodoacetate
strong inhibition in presence of Cu2+
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
depending on the metal ion used
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.43
isozyme B, isoelectric focusing
4.56
isozyme A, isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
2 isozymes A and B
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DNAS1_CHICK
282
0
31400
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30000
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
30859
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
30882
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
binds to concanavalin A, contains an N-glycosylation site
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
inactivation above
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2479fold from pancreas, isozymes A and B to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequences determination and analysis, expression in Escherichia coli BL21(DE3) in the insoluble fraction
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Elgin, S.C.R.
DNase I-hypersensitive sites of chromatin
Cell
27
413-415
1981
Gallus gallus, Drosophila melanogaster
Manually annotated by BRENDA team
Hu, C.C.; Lu, S.C.; Cheng, C.C.; Chen, L.H.; Liao, T.H.
Chicken deoxyribonuclease: purification, characterization, gene cloning and gene expression
J. Protein Chem.
22
41-49
2003
Gallus gallus (Q9YGI5), Gallus gallus
Manually annotated by BRENDA team