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EC Tree
The taxonomic range for the selected organisms is: Gallus gallus
The enzyme appears in selected viruses and cellular organisms
Synonyms
lactoferrin, dna polymerase, dnase i, colicin, diphtheria toxin, dnaase, deoxyribonuclease, dnasei, deoxyribonuclease i, crm197,
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deoxyribonuclease
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deoxyribonuclease A
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deoxyribonucleic phosphatase
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desoxyribonuclease
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endodeoxyribonuclease I
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Escherichia coli endonuclease I
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nuclease, deoxyribo-
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nuclease, Escherichia coli endo-, I
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pancreatic deoxyribonuclease
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endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
preference for double-stranded DNA
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hydrolysis of phosphoric ester
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double-stranded DNA + H2O
5'-phosphooligonucleotides + ?
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DNase I hypersensitive sites of chromatin near the 5'-ends of some genes, necessary for transcription by RNA polymerase II
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double-stranded DNA + H2O
5'-phosphooligonucleotides + ?
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DNase I hypersensitive sites of chromatin near the 5'-ends of some genes, necessary for transcription by RNA polymerase II
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?
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Mg2+
half-maximal activation at 4 mM
Mn2+
half-maximal activation at 1 mM
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2-mercaptoethanol
slight inhibition
iodoacetate
strong inhibition in presence of Cu2+
additional information
resistant to trypsin inactivation in absence of Ca2+
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additional information
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resistant to trypsin inactivation in absence of Ca2+
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additional information
purified isozymes A and B
additional information
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purified isozymes A and B
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7 - 8
depending on the metal ion used
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4.43
isozyme B, isoelectric focusing
4.56
isozyme A, isoelectric focusing
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2 isozymes A and B
SwissProt
brenda
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brenda
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brenda
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DNAS1_CHICK
282
0
31400
Swiss-Prot
Secretory Pathway (Reliability: 2)
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30000
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
30859
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
30882
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
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?
x * 30000, SDS-PAGE, x * 30859, isozyme A, mass spectrometry, x * 30882, isozyme B, mass spectrometry
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glycoprotein
binds to concanavalin A, contains an N-glycosylation site
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2479fold from pancreas, isozymes A and B to homogeneity
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DNA and amino acid sequences determination and analysis, expression in Escherichia coli BL21(DE3) in the insoluble fraction
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Elgin, S.C.R.
DNase I-hypersensitive sites of chromatin
Cell
27
413-415
1981
Gallus gallus, Drosophila melanogaster
brenda
Hu, C.C.; Lu, S.C.; Cheng, C.C.; Chen, L.H.; Liao, T.H.
Chicken deoxyribonuclease: purification, characterization, gene cloning and gene expression
J. Protein Chem.
22
41-49
2003
Gallus gallus (Q9YGI5), Gallus gallus
brenda