We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Also hydrolyses S-acetoacetylglutathione, but more slowly.
The taxonomic range for the selected organisms is: Rattus norvegicus The enzyme appears in selected viruses and cellular organisms
Synonyms
glyoxalase ii, glyoxalase 2, gly ii, glyii, glxii, glx2-2, gloii, hydroxyacylglutathione hydrolase, cgloii, glo ii,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acetoacetylglutathione hydrolase
-
-
-
-
Germ cell specific protein
-
-
-
-
hydrolase, acetoacetylglutathione
-
-
-
-
hydrolase, hydroxyacylglutathione
-
-
-
-
hydroxyacylglutathione hydrolase
-
-
Round spermatid protein RSP29
-
-
-
-
S-2-hydroxylacylglutathione hydrolase
-
-
-
-
glyoxalase II
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate
mechanism
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
hydrolysis of thioester
-
-
-
-
hydrolysis of thioester
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-(2-hydroxyacyl)glutathione hydrolase
Also hydrolyses S-acetoacetylglutathione, but more slowly.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-D-lactoylglutathione + H2O
D-lactate + glutathione
-
-
-
?
lactoylglutathione + H2O
D-lactate + glutathione
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione + H2O
beta-ethoxy-alpha-D-hydroxybutyrate + glutathione
-
-
-
-
?
S-acetoacetylglutathione + H2O
acetoacetate + glutathione
-
-
-
-
?
S-acetylglutathione + H2O
acetate + glutathione
-
-
-
-
?
S-D-lactoylglutathione + H2O
D-lactate + glutathione
-
-
-
-
?
S-formylglutathione + H2O
formate + glutathione
-
-
-
-
?
S-mandeloylglutathione + H2O
mandelate + glutathione
-
-
-
-
?
S-propionylglutathione + H2O
propionate + glutathione
-
-
-
-
?
S-succinylglutathione + H2O
succinate + glutathione
-
-
-
-
?
lactoylglutathione + H2O
D-lactate + glutathione
-
-
-
-
?
lactoylglutathione + H2O
D-lactate + glutathione
-
reaction is rate-limiting, methylglyoxal bypass of glycolysis
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-D-lactoylglutathione + H2O
D-lactate + glutathione
-
-
-
?
lactoylglutathione + H2O
D-lactate + glutathione
-
reaction is rate-limiting, methylglyoxal bypass of glycolysis
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,4,6-Trinitrobenzenesulfonate
methylene blue
-
photoinactivation
N,S-(dicarbobenzoxy)glutathione
-
-
N-acetyl-S-(p-bromobenzyl)glutathione
N-benzoyl-S-(p-bromobenzyl)glutathione
-
-
N-benzyloxycarbonyl-S-(p-bromobenzyl)glutathione
-
-
S-(p-chlorophenacyl)glutathione
-
-
S-benzyloxycarbonylglutathione
-
-
S-m-nitrobenzyloxycarbonylglutathione
-
-
S-o-nitrobenzyloxycarbonylglutathione
-
-
S-p-nitrobenzyloxycarbonylglutathione
-
-
Trinitrobenzenesulfonate
-
-
2,4,6-Trinitrobenzenesulfonate
-
-
2,4,6-Trinitrobenzenesulfonate
-
N-acetyl-S-(p-bromobenzyl)glutathione protects
diethyldicarbonate
-
-
diethyldicarbonate
-
pH 6
N-acetyl-S-(p-bromobenzyl)glutathione
-
-
N-acetyl-S-(p-bromobenzyl)glutathione
-
competitive
Phenylglyoxal
-
-
Phenylglyoxal
-
10 mM, 1 h, 60% loss of activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.044 - 0.703
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
0.413 - 0.841
S-Acetoacetylglutathione
0.568 - 0.662
S-acetylglutathione
0.384 - 0.598
S-D-lactoylglutathione
0.213
S-formylglutathione
-
cytosolic enzyme
0.18 - 0.21
S-Lactoylglutathione
0.014
S-mandeloylglutathione
0.214 - 1.2
S-propionylglutathione
0.16 - 0.535
S-succinylglutathione
0.044
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
-
cytosolic enzyme
0.05
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
-
mitochondrial enzyme
0.703
S-(beta-ethoxy-alpha-D-hydroxybutyryl)glutathione
-
mitochondrial enzyme
0.413
S-Acetoacetylglutathione
-
enzyme from intermembrane space
0.634
S-Acetoacetylglutathione
-
matrix enzyme
0.841
S-Acetoacetylglutathione
-
cytosolic enzyme
0.568
S-acetylglutathione
-
cytosolic enzyme
0.6
S-acetylglutathione
-
matrix enzyme
0.603
S-acetylglutathione
-
enzyme from intermembrane space
0.662
S-acetylglutathione
-
mitochondrial enzyme
0.384
S-D-lactoylglutathione
-
matrix enzyme
0.433
S-D-lactoylglutathione
-
cytosolic enzyme
0.44
S-D-lactoylglutathione
-
mitochondrial enzyme
0.598
S-D-lactoylglutathione
-
enzyme from intermembrane space
0.18
S-Lactoylglutathione
-
-
0.18
S-Lactoylglutathione
-
enzyme from erythrocytes
0.21
S-Lactoylglutathione
-
enzyme from liver
0.014
S-mandeloylglutathione
-
-
0.014
S-mandeloylglutathione
-
enzyme from erythrocytes
0.214
S-propionylglutathione
-
cytosolic enzyme
0.242
S-propionylglutathione
-
mitochondrial enzyme
0.378
S-propionylglutathione
-
mitochondrial enzyme
0.792
S-propionylglutathione
-
enzyme from intermembrane space
1.2
S-propionylglutathione
-
matrix enzyme
0.16
S-succinylglutathione
-
mitochondrial enzyme
0.186
S-succinylglutathione
-
enzyme from intermembrane space
0.325
S-succinylglutathione
-
cytosolic enzyme
0.535
S-succinylglutathione
-
matrix enzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
additional information
-
turnover-numver is pH-independent in the range pH 6-9.3
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2.35
-
enzyme from intermembrane space
additional information
-
activity is determined spectrophotometrically at 240 nm, the hepatic glyoxalase II activity of the 3'-methyl-4-dimethylaminoazobenzene group (10 weeks fed) is almost the same as that of the control group
additional information
-
determined spectrophotometrically at 240 nm, glyoxalase II activities of 2-methyl-4-dimethylaminoazobenzene-fed rats (10 weeks fed) are 1.3 times higher than that of the control rats
additional information
-
determined spectrophotometrically at 240 nm, glyoxalase II activities of 4'-methyl-4-dimethylaminoazobenzene-fed rats (10 weeks fed) are 1.4 times higher than that of the control rats
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
in the lung of diabetic rats, the increase in GLO I activity is not associated to a concomitant increase in GLO II activity. This finding could result in a defective methylglyoxal detoxification
brenda
-
-
brenda
-
-
brenda
-
Morris hepatoma cells 5123S
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
10-15% of the total glyoxalase II activity in liver. 56% of the activity resides in the matrix, 19% in the intermembrane space. 3 isoforms are exclusively mitochondrial
brenda
-
intermembrane space and matrix
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GLO2_RAT
309
0
34109
Swiss-Prot
Mitochondrion (Reliability: 2 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
30000
-
x * 30000, SDS-PAGE
21000
-
-
21900
-
gel filtration
21900
-
erythrocytes, gel filtration
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4
-
rapid inactivation
134176
4.5
-
stable above
134176
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
80
-
rapid inactivation at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
photoinactivation by methylene blue
-
37413, 134170, 134176
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
partial, multiple forms
-
two enzyme forms: alpha and beta
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
vander Jagt, D.L.
The glyoxalase system
Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York
3
597-641
1989
Homo sapiens, Mesocricetus auratus, Mus musculus, Rattus norvegicus
-
brenda
Van der Jagt, D.L.
Glyoxalase II: molecular characteristics, kinetic and mechanism
Biochem. Soc. Trans.
21
522-527
1993
Homo sapiens, Rattus norvegicus
brenda
Ball, J.C.; vander Jagt, D.L.
Purification of S-2-hydroxyacylglutathione hydrolase (glyoxalase II) from rat erythrocytes
Anal. Biochem.
98
472-477
1979
Rattus norvegicus
brenda
Ball, J.C.; vander Jagt, D.L.
S-2-Hydroxyacylglutathione hydrolase (glyoxalase II): active-site mapping of a nonserine thiolesterase
Biochemistry
20
899-905
1981
Rattus norvegicus
brenda
Principato, G.B.; Rosi, G.; Talesa, V.; Giovannini, E.; Uotila, L.
Purification and characterization of two forms of glyoxalase II from the liver and brain of Wistar rats
Biochim. Biophys. Acta
911
349-355
1987
Rattus norvegicus, Rattus norvegicus Wistar
brenda
Talesa, V.; Uotila, L.; Koivusalo, M.; Principato, G.; Giovannini, E.; Rosi, G.
Demonstration of glyoxalase II in rat liver mitochondria. Partial purification and occurrence in multiple forms
Biochim. Biophys. Acta
955
103-110
1988
Rattus norvegicus
brenda
Uotila, L.
Glutathione thiol esterases
Coenzymes and cofactors, Glutathione, Chem. Biochem. Med. Aspects Pt. A (Dolphin D, Poulson R, Avromonic O, eds. ) John Wiley & Sons, New York
3
767-804
1989
Bos taurus, Saccharomyces cerevisiae, Escherichia coli, Homo sapiens, Mus musculus, Rattus norvegicus
-
brenda
Talesa, V.; Uotila, L.; Koivusalo, M.; Principato, G.; Giovannini, E.; Rosi, G.
Isolation of glyoxalase II from two different compartments of rat liver mitochondria. Kinetic and immunochemical characterization of the enzymes
Biochim. Biophys. Acta
993
7-11
1989
Rattus norvegicus
brenda
Cho, M.Y.; Bae, C.D.; Park, J.B.; Lee, T.H.
Purification and cloning of glyoxalase II from rat liver
Exp. Mol. Med.
30
53-57
1998
Rattus norvegicus
brenda
Bonfigli, A.; Colafarina, S.; Falone, S.; Di Giulio, C.; Di Ilio, C.; Amicarelli, F.
High levels of antioxidant enzymatic defence assure good protection against hypoxic stress in spontaneously diabetic rats
Int. J. Biochem. Cell Biol.
38
2196-2208
2006
Rattus norvegicus (O35952)
brenda
Kitamura, Y.; Kawase, M.; Ohmori, S.
Formate excretion in urine of rats fed dimethylaminoazobenzene-rich diets: the possibility of formate formation from D-lactate
Acta Med. Okayama
62
193-203
2008
Rattus norvegicus
brenda