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EC Tree
IUBMB Comments Acts on acyl-carrier-protein thioesters of fatty acids from C12 to C18, but the derivative of oleic acid is hydrolysed much more rapidly than any other compound tested.
The taxonomic range for the selected organisms is: Ricinus communis The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
acyl-acp thioesterase, fatb1, acyl-acyl carrier protein (acp) thioesterase, fatb2, acyl-acyl carrier protein thioesterase, acyl-acp te, fatty acyl-acp thioesterase, oleoyl-acp thioesterase, mlfatb, lcfatb,
more
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acyl-acyl carrier protein (ACP) thioesterase
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12:0-ACP thioesterase
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12:0-acyl-carrier protein thioesterase
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14:0-ACP thioesterase
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14:0-acyl-carrier protein thioesterase
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16:0-ACP thioesterase
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16:0-acyl-carrier protein thioesterase
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18:0-ACP thioesterase
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18:0-acyl-carrier protein thioesterase
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acyl-ACP-hydrolase
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acyl-acyl carrier protein hydrolase
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Acyl-[acyl-carrier protein] hydrolase
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acyl-[acyl-carrier-protein] hydrolase
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acylacyl carrier protein (ACP) thioesterase
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C18:1-ACP thioesterase
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Deubiquitinating enzyme 14
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hydrolase, oleoyl-[acyl carrier protein]
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oleoyl-ACP thioesterase
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oleoyl-acyl carrier protein thioesterase
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Ubiquitin thiolesterase 14
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Ubiquitin-specific processing protease 14
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hydrolysis of thioester
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-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
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oleoyl-[acyl-carrier protein] hydrolase
Acts on acyl-carrier-protein thioesters of fatty acids from C12 to C18, but the derivative of oleic acid is hydrolysed much more rapidly than any other compound tested.
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oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
maximum catalytic efficiency of FatA
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?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
high catalytic efficiency of FatA
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-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
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?
stearoyl-[acyl-carrier protein] + H2O
stearate + acyl-carrier protein
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?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
high efficiency of FatB
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-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
high efficiency of FatB
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?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
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?
stearoyl-[acyl-carrier protein] + H2O
stearate + acyl-carrier protein
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?
additional information
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additional information
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FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives
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?
additional information
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FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives
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?
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0.00042
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.00035
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.00056
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.00054
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.00067
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.00086
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.00054
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.00088
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
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7.99
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
1.62
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.46
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.56
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.67
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.22
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.41
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
0.38
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
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19030
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
4630
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
830
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
1030
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
990
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
250
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
760
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
440
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22°C
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6.7
calculated from sequence
7
calculated from sequence
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UniProt
brenda
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brenda
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brenda
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A9XK92_RICCO
371
0
42208
TrEMBL
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37520
recombinant histidine-tagged FatA, gel filtration
42200
FatA open reading frames, calculated from sequence
36800
recombinant histidine-tagged FatB, gel filtration
46500
FatB open reading frames, calculated from sequence
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?
x * 42200, FatA open reading frames, calculated from sequence
?
x * 46500, FatA open reading frames, calculated from sequence
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to homogeneity in a single step by gel filtration
to homogeneity in a single step by gel filtration
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PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatA overexpressed in Escherichia coli XL1-Blue
PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatB overexpressed in Escherichia coli XL1-Blue
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transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation
transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation
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industry
contribution of FatA to the synthesis of castor oil
industry
contribution of FatB to the synthesis of castor oil
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Sanchez-Garcia, A.; Moreno-Perez, A.J.; Muro-Pastor, A.M.; Salas, J.J.; Garces, R.; Martinez-Force, E.
Acyl-ACP thioesterases from castor (Ricinus communis L.): An enzymatic system appropriate for high rates of oil synthesis and accumulation
Phytochemistry
71
860-869
2010
Ricinus communis (A9XK92), Ricinus communis (A9XUG5)
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